35 research outputs found

    Controlling evaporation loss from water storages

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    [Executive Summary]: Evaporation losses from on-farm storage can potentially be large, particularly in irrigation areas in northern New South Wales and Queensland where up to 40% of storage volume can be lost each year to evaporation. Reducing evaporation from a water storage would allow additional crop production, water trading or water for the environment. While theoretical research into evaporation from storages has previously been undertaken there has been little evaluation of current evaporation mitigation technologies (EMTs) on commercial sized water storages. This project was initiated by the Queensland Government Department of Natural Resources and Mines (NRM) with the express aim of addressing this gap in our knowledge. The report addressed i) assessment of the effectiveness of different EMT’s in reducing evaporation from commercial storages across a range of climate regions, ii) assessment of the practical and technical limitations of different evaporation control products, and iii) comparison of the economics of different EMT’s on water storages used for irrigation

    Histone arginine methylation in cocaine action in the nucleus accumbens

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    Repeated cocaine exposure regulates transcriptional regulation within the nucleus accumbens (NAc), and epigenetic mechanisms - such as histone acetylation and methylation on Lys residues - have been linked to these lasting actions of cocaine. In contrast to Lys methylation, the role of histone Arg (R) methylation remains underexplored in addiction models. Here we show that protein-R-methyltransferase-6 (PRMT6) and its associated histone mark, asymmetric dimethylation of R2 on histone H3 (H3R2me2a), are decreased in the NAc of mice and rats after repeated cocaine exposure, including self-administration, and in the NAc of cocaine-addicted humans. Such PRMT6 down-regulation occurs selectively in NAc medium spiny neurons (MSNs) expressing dopamine D2 receptors (D2-MSNs), with opposite regulation occurring in D1-MSNs, and serves to protect against cocaine-induced addictive-like behavioral abnormalities. Using ChIP-seq, we identified Src kinase signaling inhibitor 1 (Srcin1; also referred to as p140Cap) as a key gene target for reduced H3R2me2a binding, and found that consequent Srcin1 induction in the NAc decreases Src signaling, cocaine reward, and the motiv ation to self-administer cocaine. Taken together, these findings suggest that suppression of Src signaling in NAc D2-MSNs, via PRMT6 and H3R2me2a down-regulation, functions as a homeostatic brake to restrain cocaine action, and provide novel candidates for the development of treatments for cocaine addiction. Keywords: histone arginine (R) methylation; drug addiction; medium spiny neurons; ChIP-seq; Sr

    Circuit-wide Transcriptional Profiling Reveals Brain Region-Specific Gene Networks Regulating Depression Susceptibility

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    Depression is a complex, heterogeneous disorder and a leading contributor to the global burden of disease. Most previous research has focused on individual brain regions and genes contributing to depression. However, emerging evidence in humans and animal models suggests that dysregulated circuit function and gene expression across multiple brain regions drive depressive phenotypes. Here we performed RNA-sequencing on 4 brain regions from control animals and those susceptible or resilient to chronic social defeat stress at multiple time points. We employed an integrative network biology approach to identify transcriptional networks and key driver genes that regulate susceptibility to depressive-like symptoms. Further, we validated in vivo several key drivers and their associated transcriptional networks that regulate depression susceptibility and confirmed their functional significance at the levels of gene transcription, synaptic regulation and behavior. Our study reveals novel transcriptional networks that control stress susceptibility and offers fundamentally new leads for antidepressant drug discovery

    Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons

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    Repeated cocaine administration increases the dendritic arborization of nucleus accumbens neurons, but the underlying signaling events remain unknown. Here, we show that repeated cocaine negatively regulates the active form of Rac1, a small GTPase that controls actin remodeling in other systems. We show further, using viral-mediated gene transfer, that overexpression of a dominant negative mutant of Rac1, or local knockout of Rac1 from floxed Rac1 mice, is sufficient to increase the density of immature dendritic spines on nucleus accumbens neurons, whereas overexpression of a constitutively active Rac1 mutant, or light activation of a photoactivatible form of Rac1, blocks the ability of repeated cocaine to produce this effect. Downregulation of Rac1 activity in nucleus accumbens likewise promotes behavioral responses to cocaine, with Rac1 activation producing the opposite effect. These findings establish an important role for Rac1 signaling in mediating structural and behavioral plasticity to cocaine

    Crop Updates 2006 - Lupins and Pulses

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    This session covers sixty six papers from different authors: 2005 LUPIN AND PULSE INDUSTRY HIGHLIGHTS 1. Lupin Peter White, Department of Agriculture 2. Pulses Mark Seymour, Department of Agriculture 3. Monthly rainfall at experimental sites in 2005 4. Acknowledgements Amelia McLarty EDITOR 5. Contributors 6. Background Peter White, Department of Agriculture 2005 REGIONAL ROUNDUP 7. Northern agricultural region Wayne Parker, Department of Agriculture 8. Central agricultural region Ian Pritchard and Bob French, Department of Agriculture 9. Great southern and lakes Rodger Beermier, Department of Agriculture 10. South east region Mark Seymour, Department of Agriculture LUPIN AND PULSE PRODUCTION AGRONOMY AND GENETIC IMPROVEMENT 11. Lupin Peter White, Department of Agriculture 12. Narrow-leafed lupin breeding Bevan Buirchell, Department of Agriculture 13. Progress in the development of pearl lupin (Lupinus mutabilis) for Australian agriculture, Mark Sweetingham1,2, Jon Clements1, Geoff Thomas2, Roger Jones1, Sofia Sipsas1, John Quealy2, Leigh Smith1 and Gordon Francis1 1CLIMA, The University of Western Australia 2Department of Agriculture 14. Molecular genetic markers and lupin breeding, Huaan Yang, Jeffrey Boersma, Bevan Buirchell, Department of Agriculture 15. Construction of a genetic linkage map using MFLP, and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus augustiflolius L) Jeffrey Boersma1,2, Margaret Pallotta3, Bevan Buirchell1, Chengdao Li1, Krishnapillai Sivasithamparam2 and Huaan Yang1 1Department of Agriculture, 2The University of Western Australia, 3Australian Centre for Plant Functional Genomics, South Australia 16. The first gene-based map of narrow-leafed lupin – location of domestication genes and conserved synteny with Medicago truncatula, M. Nelson1, H. Phan2, S. Ellwood2, P. Moolhuijzen3, M. Bellgard3, J. Hane2, A. Williams2, J. Fos‑Nyarko4, B. Wolko5, M. Książkiewicz5, M. Cakir4, M. Jones4, M. Scobie4, C. O’Lone1, S.J. Barker1, R. Oliver2, and W. Cowling1 1School of Plant Biology, The University of Western Australia, 2Australian Centre for Necrotrophic Fungal Pathogens, Murdoch University, 3Centre for Bioinformatics and Biological Computing, Murdoch University, 4School of Biological Sciences and Biotechnology, SABC, Murdoch University,5Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland 17. How does lupin optimum density change row spacing? Bob French and Laurie Maiolo, Department of Agriculture 18. Wide row spacing and seeding rate of lupins with conventional and precision seeding machines Martin Harries, Jo Walker and Murray Blyth, Department of Agriculture 19. Influence of row spacing and plant density on lupin competition with annual ryegrass, Martin Harries, Jo Walker and Murray Blyth, Department of Agriculture 20. Effect of timing and speed of inter-row cultivation on lupins, Martin Harries, Jo Walker and Steve Cosh, Department of Agriculture 21. The interaction of atrazine herbicide rate and row spacing on lupin seedling survival, Martin Harries and Jo Walker Department of Agriculture 22. The banding of herbicides on lupin row crops, Martin Harries, Jo Walker and Murray Blyth, Department of Agriculture 23. Large plot testing of herbicide tolerance of new lupin lines, Wayne Parker, Department of Agriculture 24. Effect of seed source and simazine rate of seedling emergence and growth, Peter White and Greg Shea, Department of Agriculture 25. The effect of lupin row spacing and seeding rate on a following wheat crop, Martin Harries, Jo Walker and Dirranie Kirby, Department of Agriculture 26. Response of crop lupin species to row spacing, Leigh Smith1, Kedar Adhikari1, Jon Clements2 and Patrizia Guantini3, 1Department of Agriculture, 2CLIMA, The University of Western Australia, 3University of Florence, Italy 27. Response of Lupinus mutabilis to lime application and over watering, Peter White, Leigh Smith and Mark Sweetingham, Department of Agriculture 28. Impact of anthracnose on yield of Andromeda lupins, Geoff Thomas, Kedar Adhikari and Katie Bell, Department of Agriculture 29. Survey of lupin root health (in major production areas), Geoff Thomas, Ken Adcock, Katie Bell, Ciara Beard and Anne Smith, Department of Agriculture 30. Development of a generic forecasting and decision support system for diseases in the Western Australian wheatbelt, Tim Maling1, Art Diggle1,2, Debbie Thackray1, Kadambot Siddique1 and Roger Jones1,2 1CLIMA, The University of Western Australia, 2Department of Agriculture 31.Tanjil mutants highly tolerant to metribuzin, Ping Si1, Mark Sweetingham1,2, Bevan Buirchell1,2 and Huaan Yang l,2 1CLIMA, The University of Western Australia, 2Department of Agriculture 32. Precipitation pH vs. yield and functional properties of lupin protein isolate, Vijay Jayasena1, Hui Jun Chih1 and Ken Dods2 1Curtin University of Technology, 2Chemistry Centre 33. Lupin protein isolation with the use of salts, Vijay Jayasena1, Florence Kartawinata1,Ranil Coorey1 and Ken Dods2 1Curtin University of Technology, 2Chemistry Centre 34. Field pea, Mark Seymour, Department of Agriculture 35. Breeding highlights Kerry Regan1,2, Tanveer Khan1,2, Stuart Morgan1 and Phillip Chambers1 1Department of Agriculture, 2CLIMA, The University of Western Australia 36. Variety evaluation, Kerry Regan1,2, Tanveer Khan1,2, Jenny Garlinge1 and Rod Hunter1 1Department of Agriculture, 2CLIMA, The University of Western Australia 37. Days to flowering of field pea varieties throughout WA Mark Seymour1, Ian Pritchard1, Rodger Beermier1, Pam Burgess1 and Dr Eric Armstrong2 Department of Agriculture, 2NSW Department of Primary Industries, Wagga Wagga 38. Semi-leafless field peas yield more, with less ryegrass seed set, in narrow rows, Glen Riethmuller, Department of Agriculture 39. Swathing, stripping and other innovative ways to harvest field peas, Mark Seymour, Ian Pritchard, Rodger Beermier and Pam Burgess, Department of Agriculture 40. Pulse demonstrations, Ian Pritchard, Wayne Parker, Greg Shea, Department of Agriculture 41. Field pea extension – focus on field peas 2005, Ian Pritchard, Department of Agriculture 42. Field pea blackspot disease in 2005: Prediction versus reality, Moin Salam, Jean Galloway, Pip Payne, Bill MacLeod and Art Diggle, Department of Agriculture 43. Pea seed-borne mosaic virus in pulses: Screening for seed quality defects and virus resistance, Rohan Prince, Brenda Coutts and Roger Jones, Department of Agriculture, and CLIMA, The University of Western Australia 44. Yield losses from sowing field peas infected with pea seed-borne mosaic virus, Rohan Prince, Brenda Coutts and Roger Jones, Department of Agriculture, and CLIMA, The University of Western Australia 45. Desi chickpea, Wayne Parker, Department of Agriculture 46. Breeding highlights, Tanveer Khan 1,2, Pooran Gaur3, Kadambot Siddique2, Heather Clarke2, Stuart Morgan1and Alan Harris1, 1Department of Agriculture2CLIMA, The University of Western Australia, 3International Crop Research Institute for Semi Arid Tropics (ICRISAT), India 47. National chickpea improvement program, Kerry Regan1, Ted Knights2 and Kristy Hobson3,1Department of Agriculture, 2Agriculture New South Wales 3Department of Primary Industries, Victoria 48. Chickpea breeding lines in CVT exhibit excellent ascochyta blight resistance, Tanveer Khan1,2, Alan Harris1, Stuart Morgan1 and Kerry Regan1,2, 1Department of Agriculture, 2CLIMA, The University of Western Australia 49. Variety evaluation, Kerry Regan1,2, Tanveer Khan1,2, Jenny Garlinge2 and Rod Hunter2, 1CLIMA, The University of Western Australia 2Department of Agriculture 50. Desi chickpeas for the wheatbelt, Wayne Parker and Ian Pritchard, Department of Agriculture 51. Large scale demonstration of new chickpea varieties, Wayne Parker, MurrayBlyth, Steve Cosh, Dirranie Kirby and Chris Matthews, Department of Agriculture 52. Ascochyta management with new chickpeas, Martin Harries, Bill MacLeod, Murray Blyth and Jo Walker, Department of Agriculture 53. Management of ascochyta blight in improved chickpea varieties, Bill MacLeod1, Colin Hanbury2, Pip Payne1, Martin Harries1, Murray Blyth1, Tanveer Khan1,2, Kadambot Siddique2, 1Department of Agriculture, 2CLIMA, The University of Western Australia 54. Botrytis grey mould of chickpea, Bill MacLeod, Department of Agriculture 55. Kabuli chickpea, Kerry Regan, Department of Agriculture, and CLIMA, The University of Western Australia 56. New ascochyta blight resistant, high quality kabuli chickpea varieties, Kerry Regan1,2, Kadambot Siddique2, Tim Pope2 and Mike Baker1, 1Department of Agriculture, 2CLIMA, The University of Western Australia 57. Crop production and disease management of Almaz and Nafice, Kerry Regan and Bill MacLeod, Department of Agriculture, and CLIMA, The University of Western Australia 58. Faba bean,Mark Seymour, Department of Agriculture 59. Germplasm evaluation – faba bean, Mark Seymour1, Tim Pope2, Peter White1, Martin Harries1, Murray Blyth1, Rodger Beermier1, Pam Burgess1 and Leanne Young1,1Department of Agriculture, 2CLIMA, The University of Western Australia 60. Factors affecting seed coat colour of faba bean during storage, Syed Muhammad Nasar-Abbas1, Julie Plummer1, Kadambot Siddique2, Peter White 3, D. Harris4 and Ken Dods4.1The University of Western Australia, 2CLIMA, The University of Western Australia, 3Department of Agriculture, 4Chemistry Centre 61. Lentil,Kerry Regan, Department of Agriculture, and CLIMA, The University of Western Australia 62. Variety and germplasm evaluation, Kerry Regan1,2, Tim Pope2, Leanne Young1, Phill Chambers1, Alan Harris1, Wayne Parker1 and Michael Materne3, 1Department of Agriculture 2CLIMA, The University of Western Australia, 3Department of Primary Industries, Victoria Pulse species 63. Land suitability for production of different crop species in Western Australia, Peter White, Dennis van Gool, and Mike Baker, Department of Agriculture 64. Genomic synteny in legumes: Application to crop breeding, Huyen Phan1, Simon Ellwood1, J. Hane1, Angela Williams1, R. Ford2, S. Thomas3 and Richard Oliver1,1Australian Centre of Necrotrophic Plant Pathogens, Murdoch University 2BioMarka, School of Agriculture and Food Systems, ILFR, University of Melbourne 3NSW Department of Primary Industries 65. ALOSCA – Development of a dry flow legume seed inoculant, Rory Coffey and Chris Poole, ALOSCA Technologies Pty Ltd 66. Genetic dissection of resistance to fungal necrotrophs in Medicago truncatula, Simon Ellwood1, Theo Pfaff1, Judith Lichtenzveig12, Lars Kamphuis1, Nola D\u27Souza1, Angela Williams1, Emma Groves1, Karam Singh2 and Richard Oliver1 1Australian Centre of Necrotrophic Plant Pathogens, Murdoch University, 2CSIRO Plant Industry APPENDIX I: LIST OF COMMON ACRONYM

    Evaporation control using covers - some realistic solutions for the irrigation industry

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    [Abstract]: In Australia, up to 40% of the water stored in small farm dams over the summer months may be lost to evaporation. Reducing this loss using either physical or chemical covers therefore has the potential to significantly increase agricultural water use efficiency in Australia and therefore generate millions of dollars for the Australian economy. A research project took place from 2003-2005 to investigate the performance of various different types of cover. Outcomes from the Queensland Natural Resources and Mines commissioned project included important new knowledge regarding the field performance of various different types of cover, technology to accurately assess evaporation and seepage losses and increased public awareness of the potential for evaporation reduction on water storages. Significant interest has been shown by landholders, agencies and consultancy companies in developing this further. The project involved the development of a novel experimental method to measure and record water depth to an accuracy of ±1mm using highly accurate Pressure Sensitive Transducers (Craig et al 2005). PST units were placed at a constant 30cm height above the dam floor by a float-weight mechanism. The units were connected to dataloggers and the data was compared to weather station derived Penman-Monteith based estimates of evaporation. This enabled evaporation losses to be separated from seepage, also a very significant loss of Australian farm water. The PST methodology confirmed that evaporation losses in small farm dams was typically 4-7mm/day in summer rising to 10mm/day when air temperatures exceeded 40° C. As a matter of interest, the analysis also revealed that Australian summer night-time evaporation due to heat advection effects could be as much as 10-20% of the total daily evaporation. The PST analysis technique applied to covered dams revealed evaporation reduction performance figures of approximately 75% for shadecloth covered dams, up to 95% with dams covered with a properly functioning floating cover, and approximately 15 to 30% with dams covered with a cetyl alcohol based chemical monolayer (Barnes, 1993). Optimising the performance of monolayers with improved application technology is currently the focus of evaporation research efforts at NCEA. The study has revealed that high evaporation savings are possible if physical covers are used on small farm dams less than 10ha in size. Physical covers can still be used on dams larger than 10ha in size, but may be prove economically impractical due to the high amounts of capital investment required. In the future, however, increasing cost of water may allow increasingly large sizes of dam to be covered. For the present, economic analyses have implied that chemical covers may represent the best option for evaporation control on large farm water storages. Future research is planned commencing in June 2006 with the support of the CRC for Irrigation Futures and collaboration with CRC for Polymers and Nylex Water Solutions, producers of WaterSavr, the cetyl alcohol based chemical monolayer. A new applicator for applying the monolayer has recently been developed which relies on a compressed air system for delivery of the powder just below the water surface. The instrument was successfully demonstrated for the first time in Australia at USQ Toowoomba in the presence of David Verlee (Vice President of Business Development, Water Savr Global Solutions Inc, Richmond, Canada and Greg Phillips, Northern Regional Manager, Nylex Corporation. The applicator has been trialed at Byrock (80km south of Bourke, NSW) by Brendon Mason, Nylex Water Solutions. The research will focus upon developing computer assisted application technology which will relies upon visualisation of the chemical coverage on the water surface. Fundamental research will be carried out on the performance of chemical monolayers in terms of their spread and resistance to breakup, microbial and UV degradation. Preliminary investigations have indicated that the performance of cetyl alcohol based monolayers may be significantly enhanced with the addition of other chemicals, for example, poly vinyl stearate. PVS is a polymer with a comb-like structure which may enhance the resistance of the monolayer to wind stress (Barnes, 1993). The NCEA evaporation research team comprises Director Erik Schmidt, Ian Craig, Andrew Green and Michael Scobie. The project was funded by the Queensland Department of Natural Resources and Mines (NRM) through the Rural Water Use Efficiency Initiative (RWUEI). The project depended upon the cooperation of product suppliers including NetPro (shadecloth), Nylex Water Solutions (WaterSavr), Evaporation Control Systems (E-VapCap), Raftex, Ciba Speciality Chemicals, and site owners Moonrocks (St George), Cubbie Station (Dirranbandi), Renato Andreatta (Stanthorpe) and Peak Downs Shire Council (Capella)

    A Study of the Inter-Relationships Between Ketosis and Leucine, Alanine and Glucose Metabolism in Normal and Obese Fasted Human Subjects Using Tracer Methodology

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    1. This thesis describes a quantitative investigation of leucine, alanine and glucose turnover, metabolite concentrations and hormone levels (insulin, C-peptide, cortisol and glucagon) in normal weight subjects studied after 12 hours and 60 hours of starvation and in obese subjects studied after 12 hours, 60 hours and 2 weeks of starvation. Leucine turnover was measured with L-[1-14C] leucine, alanine turnover with L-[1-14C] alanine and glucose turnover with D-[3-3H] glucose

    An atlas of diabetes mellitus

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    Buku ini memberikan latar belakang klinis dan ilmiah untuk diagnosis, presentasi klinis, dan pengobatan diabetes mellitus. Tujuan lebih lanjut dari buku ini adalah untuk menggambarkan ekspresi diabetes yang luas dan bervariasi serta komplikasinya sebagai bantuan untuk pengenalan yang lebih siap dalam praktik klinis. Karenanya harus menjadi perhatian semua orang yang terlibat dalam penyediaan perawatan kesehatan diabetes.Boca Raton100 hlm.: ill., index; 30x21c

    Controlling evaporation from on-farm storages

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    The National Centre for Engineering in Agriculture (NCEA), University of Southern Queensland (USQ) has for the last two years been assessing different techniques for suppressing evaporation from storages. The project has been funded by the Queensland Government’s Department of Natural Resources and Mines through their Rural Water Use Efficiency Initiative (RWUEI). Evaporation Mitigation Technologies (EMT) assessed included chemical monolayers, floating covers, modules and suspended shade structures. The EMTs were investigated according to their efficiency in reducing evaporation, durability in the field and economic practicality
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