Detection of Mercury Among Avian Trophic Levels at Caddo Lake and Lake Lewisville, TX

Mercury (Hg) is a globally distributed toxicant that has been shown to have negative effects on birds. in the United States, avian taxa have been shown to possess high Hg concentrations in the northeast, Great Lakes and Everglades ecosystems; however, few studies have measured avian Hg concentrations in other geographic regions. Previous studies have documented high Hg concentrations in multiple organisms in east Texas, but birds were not included in these studies. the main objective of the present study was to quantify Hg concentrations in birds in differing trophic levels at Caddo Lake and Lake Lewisville, TX. Results suggest that Hg concentrations may be high enough to negatively impact some bird taxa, particularly those at high trophic levels, residing at both Caddo Lake and Lake Lewisville

Can Increased Training and Awareness Take Forest Research to New Heights?

Forest canopies contribute significantly to global forest biodiversity and ecosystem functioning, yet are declining and understudied. One reason for a knowledge gap is that accessing forest canopies can be difficult and dangerous. Thus, lack of relevant canopy access skills may compromise knowledge gain and personal safety. We assessed skill levels in canopy access methods and self-perception of skills amongst ecologists worldwide via a web-based survey, available in four languages. We obtained responses from expert arborists as a control group. From 191 respondents who said canopy access is relevant to their research (of 1,070 total responses), we found that ecologists are not attaining the full potential provided by existing methods of canopy access. Specifically, most respondents are unable to access much of the forest canopy, especially areas away from the trunk and between trees. The survey further revealed the common use of unsafe and inefficient practices among ecologists and that few are adequately equipped with aerial rescue skills. Importantly, ecologists with the lowest skill levels overestimate their expertise the most. Proper field techniques are key components of good science: they can improve study design, increase potential for data collection, and ultimately reveal greater knowledge on canopy organisms and processes. By safely allowing greater access to the forest canopy, proper techniques can reduce bias in our scientific understanding of forest ecology. To facilitate safe and effective canopy access for ecological research, we recommend increasing instruction and collaboration, implementing certification programs, and conducting audits of canopy research programs. With increased access to such opportunities, ecologists will acquire improved skills in accessing forest canopies, develop a greater appreciation for the full breadth of possibilities among methods of canopy access, and more safely and effectively gather the data needed to better understand forest ecosystems

Commentary: Research Recommendations for Understanding the Decline of American Kestrels (\u3cem\u3eFalco sparverius\u3c/em\u3e) Across Much of North America

Across much of North America, populations of American Kestrels (Falco sparverius) have been in decline for decades (Farmer et al. 2008, Farmer and Smith 2009, Smallwood et al. 2009a, Paprocki et al. 2014, Sauer et al. 2014). Hypothesized causes of kestrel declines include predation by Cooper\u27s Hawks (Accipiter cooperii; Farmer et al. 2008), pathogens (e.g., Nemeth et al. 2006), habitat loss (Sullivan and Wood 2005, Farmer et al. 2008, Bolgiano et al. 2015), pesticides (Smallwood et al. 2009a, Rattner et al. 2015), and climate change (Steenhof and Peterson 2009b), yet no hypothesized factor has been supported empirically (Farmer et al. 2006, Smallwood et al. 2009a). Despite the effort spent evaluating threats, the lack of a “smoking-gun” to explain the decline of this charismatic species has led many professional and citizen scientists to call for action on several unlikely, and unsupported, threats. Here, we evaluate and build on hypothesized causes of declines considered by other authors (e.g., Sullivan and Wood 2005, Farmer et al. 2008, Smallwood et al. 2009a) to synthesize conclusions and articulate research needs

Structural characterization of antibiotic self-immunity tRNA synthetase in plant tumour biocontrol agent

Antibiotic-producing microbes evolved self-resistance mechanisms to avoid suicide. The biocontrol Agrobacterium radiobacter K84 secretes the Trojan Horse antibiotic agrocin 84 that is selectively transported into the plant pathogen A. tumefaciens and processed into the toxin TM84. We previously showed that TM84 employs a unique tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase (LeuRS), while the TM84-producer prevents self-poisoning by expressing a resistant LeuRS AgnB2. We now identify a mechanism by which the antibiotic-producing microbe resists its own toxin. Using a combination of structural, biochemical and biophysical approaches, we show that AgnB2 evolved structural changes so as to resist the antibiotic by eliminating the tRNA-dependence of TM84 binding. Mutagenesis of key resistance determinants results in mutants adopting an antibiotic-sensitive phenotype. This study illuminates the evolution of resistance in self-immunity genes and provides mechanistic insights into a fascinating tRNA-dependent antibiotic with applications for the development of anti-infectives and the prevention of biocontrol emasculation

Phenology Effects on Productivity and Hatching-Asynchrony of American Kestrels (\u3cem\u3eFalco sparverius\u3c/em\u3e) Across a Continent

Optimal reproductive performance occurs when birds time reproduction to coincide with peak food availability. Deviation from optimal timing, or mismatch, can affect productivity, though birds may mediate some mismatch effects by altering their incubation behavior. We studied the consequences of nesting timing (i.e., clutch initiation relative to an index of spring start) on productivity across the breeding range of American kestrels (Falco sparverius) in the United States and southern Canada, and associations between nesting timing, incubation behavior, and hatching asynchrony. We used observations from long-term nest box monitoring, remote trail cameras, and community-scientist-based programs to obtain data on clutch initiation, productivity, incubation, and hatching synchrony. Kestrels that initiated clutches after the extended spring index (SI-x, start of spring estimate) had higher rates of nest failure and fewer nestlings than earlier nesters, and effects of nesting timing on productivity were strongest in the Northeast. In contrast, kestrels in the Southwest experienced a more gradual decline in productivity across the season. Spatial effects may be the result of regional differences in growing seasons and temporal nesting windows (duration of nesting season). Specifically, resource availability in the Northeast was highly peaked during the breeding season, potentially resulting in shorter nesting windows. Conversely, resource curves were more prolonged in the Southwest, and growing seasons are becoming longer with climate change, potentially resulting in longer nesting windows. We found an inverse relationship between nesting timing and the onset of male incubation. Males from breeding pairs that initiated clutches after SI-x began incubation sooner than males from breeding pairs that initiated clutches before SI-x. Early-onset of male incubation was positively associated with hatching asynchrony, creating increased age variation in developing young. In sum, nesting phenology relative to the SI-x has consequences for American kestrels’ productivity, and these consequences vary across space. The early onset of incubation may act as a potential adaptive behavior to advance the average hatch date and spread out energetic demands. Given the effects of nesting timing on productivity, kestrels are likely to be sensitive to climate-driven advances in growing seasons and vulnerable to phenological mismatch, particularly in the Northeast

Characteristics of US-Based STEM Webcams \u3cem\u3eat a Glance\u3c/em\u3e

STEM organizations operate thousands of publicly available webcams, which have the potential to provide rich informal learning opportunities. To date, no research has analyzed the breadth of STEM webcams. In our study, we developed an inventory by performing internet searches for US-based STEM webcams and recorded operator name, organization type, webcam subject, etc. This inventory serves as the basis for a follow-up survey of webcam operators, which seeks to better understand the goals, outcomes, and investments of STEM webcam programs. We characterized nearly 1000 webcams that fit within our inclusion criteria. The majority of the cams fell within the life sciences or geosciences. Of the animal webcams, mammals (60%) and birds (23%) were strongly represented in contrast to reptiles (2%) and insects (4%). Within the mammal subjects, tigers, elephants, otters, and giraffes were most common (5-7% each). Within the 23 different bird webcam subjects, penguins (30%) and osprey (20%) were most common. Other studies have shown the over-representation of charismatic animals (e.g., elephants, giraffes, tigers, bears) in zoos, and our study suggests that webcams may amplify this phenomenon. Further, these preliminary results suggest that life sciences topics dominate STEM webcams, leaving potential for other visually engaging fields to participate

Informing Conservation Management Using Genetic Approaches: Greater Sage-Grouse and Galápagos Short-Eared Owls as Case Studies

Small isolated populations are of particular conservation interest due to their increased extinction risk. This dissertation investigates two small wild bird populations using genetic approaches to inform their conservation. Specifically, one case study investigated a Greater Sage-grouse (Centrocercus urophasianus) population located in northwest Wyoming near Jackson Hole and Grand Teton National Park. Microsatellite data showed that the Jackson sage-grouse population possessed significantly reduced levels of neutral genetic diversity and was isolated from other Wyoming populations. Analysis with single nucleotide polymorphisms (SNPs) and microsatellite data provided further evidence that the population's timing of isolation was relatively recent and most likely due to recent anthropogenic habitat changes. Conservation recommendations include maintaining or increasing the population's current size and reestablishing gene flow with the nearest large population. The second case study investigated the genetic distinctiveness of the Floreana island population of the Galápagos Short-eared Owl (Asio flammeus galapagoensis). Mitochondrial DNA sequence data did not detect differences across nine island populations, yet microsatellite and morphometric data indicated that limited gene flow existed with the population and surrounding island populations, which appeared asymmetric in direction from Floreana to Santa Cruz with no indication of gene flow into Floreana. These results have important conservation implications and recommend that the Floreana Short-eared Owl population be held in captivity during the rodenticide application planned for an ecosystem restoration project in 2018. The population is less likely to receive immigrants from surrounding island populations if negatively effected by feeding on poisoned rodents

Low neutral genetic diversity in isolated Greater Sage-Grouse (Centrocercus urophasianus) populations in northwest Wyoming

This article contains an analysis of 16 microstatellite loci from 300 Greater Sage-Grouse individuals to assess genetic structure among populations in Wyoming and southeast Montana

A Regional and Continental Scale Analysis of Orientation and Nest Box Occupancy by American Kestrels

The American Kestrel (Falco sparverius), the world’s smallest falcon, breeds across North America. Kestrels readily uses nest boxes to lay their eggs in because they are secondary cavity nesters. This means that kestrels do not make the cavities that they nest in, but rather nest in cavities made by other species prior. Previous studies that have investigated the relationship between American Kestrel box occupancy and box entrance orientation in North America have had differing results. Professional and citizen scientists across the continent have installed thousands nest boxes for use by kestrels during the breeding season. Many have shared their data regarding nest box characteristics (e.g., location, nest box orientation, height, etc.) and occupancy with The Peregrine Fund’s American Kestrel Partnership (AKP). We analyzed AKP data to determine if there was an association between box occupancy by kestrels and box entrance orientation. Preliminary findings suggest that, at the continental scale, kestrels use boxes in proportion to their orientation for all orientations except the southeast. At the continental scale, American Kestrels occupied southeast facing boxes significantly less than expected based on their availability. The underlying driver of this pattern is unclear and deserves further investigation because kestrel populations are in decline