18,231 research outputs found
Dissection of Nodule Development by Supplementation of \u3cem\u3eRhizobium leguminosarum\u3c/em\u3e biovar \u3cem\u3ephaseoli\u3c/em\u3e Purine Auxotrophs with 4-Aminoimidazole-5-Carboxamide Riboside
Purine auxotrophs of Rhizobium leguminosarum biovar phaseoli CFN42 elicit uninfected pseudonodules on bean (Phaseolus vulgaris L.). Addition of 4-aminoimidazole-5-carboxamide (AICA) riboside to the root medium during incubation of the plant with these mutants leads to enhanced nodule development, although nitrogenase activity is not detected. Nodules elicited in this manner had infection threads and anatomical features characteristic of normal nodules, such as peripheral vasculature rather than the central vasculature of the pseudonodules that were elicited without AICA riboside supplementation. Although 105 to 106 bacteria could be recovered from these nodules after full development, bacteria were not observed in the interior nodule cells. Instead, large cells with extensive internal membranes were present. Approximately 5% of the normal amount of leghemoglobin and 10% of the normal amount of uricase were detected in these nodules. To promote the development of true nodules rather than pseudonodules, AICA riboside was required no later than the second day through no more than the sixth day following inoculation. After this period, removal of AICA riboside from the root medium did not prevent the formation of true nodules. This observation suggests that there is a critical stage of infection, reached before nodule emergence, at which development becomes committed to forming a true nodule rather than a pseudonodule
Lattice structure and magnetization of LaCoO3 thin films
We investigate the structure and magnetic properties of thin films of the
LaCoO compound. Thin films are deposited by pulsed laser deposition on
various substrates in order to tune the strain from compressive to tensile.
Single-phase (001) oriented LaCoO layers were grown on all substrates
despite large misfits. The tetragonal distortion of the films covers a wide
range from -2% to 2.8%. Our LaCoO films are ferromagnetic with Curie
temperature around 85 K, contrary to the bulk. The total magnetic moment is
below /Co, a value relatively small for an exited spin-state
of the Co ions, but comparable to values reported in literature. A
correlation of strain states and magnetic moment of Co ions in
LaCoO thin films is observed.Comment: submitted tu European Phys. J.
Strain-induced insulator state in La_0.7Sr_0.3CoO_3
We report on the observation of a strain-induced insulator state in
ferromagnetic La_0.7Sr_0.3CoO_3 films. Tensile strain above 1% is found to
enhance the resistivity by several orders of magnitude. Reversible strain of
0.15% applied using a piezoelectric substrate triggers huge resistance
modulations, including a change by a factor of 10 in the paramagnetic regime at
300 K. However, below the ferromagnetic ordering temperature, the magnetization
data indicate weak dependence on strain for the spin state of the Co ions. We
interpret the changes observed in the transport properties in terms of a
strain-induced splitting of the Co e_g levels and reduced double exchange,
combined with a percolation-type conduction in an electronic cluster state
Properties of optically selected BL Lac candidates from the SDSS
\textbf{Context.} Deep optical surveys open the avenue for find large numbers
of BL Lac objects that are hard to identify because they lack the unique
properties classifying them as such. While radio or X-ray surveys typically
reveal dozens of sources, recent compilations based on optical criteria alone
have increased the number of BL Lac candidates considerably. However, these
compilations are subject to biases and may contain a substantial number of
contaminating sources. \textbf{Aims.} In this paper we extend our analysis of
182 optically selected BL Lac object candidates from the SDSS with respect to
an earlier study. The main goal is to determine the number of bona fide BL Lac
objects in this sample. \textbf{Methods.} We examine their variability
characteristics, determine their broad-band radio-UV SEDs, and search for the
presence of a host galaxy. In addition we present new optical spectra for 27
targets with improved S/N with respect to the SDSS spectra. \textbf{Results.}
At least 59% of our targets have shown variability between SDSS DR2 and our
observations by more than 0.1-0.27 mag de- pending on the telescope used. A
host galaxy was detected in 36% of our targets. The host galaxy type and
luminosities are consistent with earlier studies of BL Lac host galaxies.
Simple fits to broad-band SEDS for 104 targets of our sample derived
synchrotron peak frequencies between with a peak at
. Our new optical spectra do not reveal any new
redshift for any of our objects. Thus the sample contains a large number of
bona fide BL Lac objects and seems to contain a substantial fraction of
intermediate-frequency peaked BL Lacs.Comment: Accepted for publication in A\&
Infection of Soybean and Pea Nodules by \u3cem\u3eRhizobium\u3c/em\u3e spp. Purine Auxotrophs in the Presence of 5-aminoimidazole-4-Carboxamide Riboside
Purine auxotrophs of various Rhizobium species are symbiotically defective, usually unable to initiate or complete the infection process. Earlier studies demonstrated that, in the Rhizobium etli-bean symbiosis, infection by purine auxotrophs is partially restored by supplementation of the plant medium with 5-amino-imidazole-4-carboxamide (AICA) riboside, the unphosphorylated form of the purine biosynthetic intermediate AICAR. The addition of purine to the root environment does not have this effect. In this study, purine auxotrophs of Rhizobium fredii HH303 and Rhizobium leguminosarum 128C56 (bv. viciae) were examined. Nutritional and genetic characterization indicated that each mutant was blocked in purine biosynthesis prior to the production of AICAR. R. fredii HH303 and R. leguminosarum 128C56 appeared to be deficient in AICA riboside transport and/or conversion into AICAR, and the auxotrophs derived from them grew very poorly with AICA riboside as a purine source. All of the auxotrophs elicited poorly developed, uninfected nodules on their appropriate hosts. On peas, addition of AICA riboside or purine to the root environment led to enhanced nodulation; however, infection threads were observed only in the presence of AICA riboside. On soybeans, only AICA riboside was effective in enhancing nodulation and promoting infection. Although AICA riboside supplementation of the auxotrophs led to infection thread development on both hosts, the numbers of bacteria recovered from the nodules were still 2 or more orders of magnitude lower than in fully developed nodules populated by wild-type bacteria. The ability to AICA riboside to promote infection by purine auxotrophs, despite serving as a very poor purine source for these strains, supports the hypothesis that AICAR plays a role in infection other than merely promoting bacterial growth
Reversible strain effect on the magnetization of LaCoO3 films
The magnetization of ferromagnetic LaCoO3 films grown epitaxially on
piezoelectric substrates has been found to systematically decrease with the
reduction of tensile strain. The magnetization change induced by the reversible
strain variation reveals an increase of the Co magnetic moment with tensile
strain. The biaxial strain dependence of the Curie temperature is estimated to
be below 4K/% in the as-grown tensile strain state of our films. This is in
agreement with results from statically strained films on various substrates
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