Resonance energy transfer studies of the mechanisms of microclustering of lentil lectin membrane receptors on HeLa cells

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Evidence for reversible microclustering of lentil lectin membrane receptors on HeLa cells

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

A fluorometric assay for red blood cell antibodies

A fluorometric assay is described for the detection of red blood cell antibodies. The assay reveals as little as 600 molecules of bound, fluoresceinated rabbit anti-human IgG antibodies per erythrocyte. Eleven patients with possible autoimmune erythrocyte disorder and negative direct antiglobulin test were studied by the fluorometric assay. The outcome of the fluorometric assay was compared with that of the human allogeneic rosette test. Results obtained by the two methods were in complete agreement. Five of the patients were shown to possess unexpectedly high levels of erythrocyte-bound IgG in spite of a negative, direct antiglobulin test. These findings and the validity of the fluorometric assay are discussed.SCOPUS: ar.jFLWNAinfo:eu-repo/semantics/publishe

Switched allotype expression in an immunoglobulin‐nonsecreting rabbit lymphoid cell line fused with rabbit gangliocytes

The Simian virus 40‐transformed rabbit spleen cell TRSC‐1 synthesizes intracellular whole IgG molecules of the a1b4 allotype. Two hypoxanthine‐guanine phosphoryl transferase‐deficient mutants were derived from this line. One of these, TRSC‐1‐8, was used in somatic cell fusion experiments together with gangliocytes from a rabbit immunized against β‐galactosidase. Out of nineteen hybrid clones surviving in selective medium, only one, L17, was shown to produce free γ chains which express the a2 allotype of the donor rabbit rather than the a1 marker of the parents TRSC‐1‐8 line. The inability to restore IgG secretion in hybrids suggests that dominant regulatory controls are exerted by the TRSC‐1 genome on Ig production. This supports the notion that the TRSC‐1 line originated from a splenocyte that had not reached the final plasmocyte differentiation stage at the time of viral transformation. Copyright © 1981 WILEY‐VCH Verlag GmbH & Co. KGaA, WeinheimSCOPUS: ar.jFLWNAinfo:eu-repo/semantics/publishe