Evaluation of minor pathogen intramammary infection, susceptibility parameters, and somatic cell counts on the development of new intramammary infections with major mastitis pathogens

Major mastitis pathogens such as Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and coliforms are usually considered more virulent and damaging to the udder than minor mastitis pathogens such as Corynebacterium spp. and coagulase-negative staphylococci (CNS). The current literature comprises several studies (n=38) detailing analyses with conflicting results as to whether intramammary infections (IMI) with the minor pathogens decrease, increase, or have no effect on the risk of a quarter acquiring a new IMI (NIMI) with a major pathogen. The Canadian Bovine Mastitis Research Network has a large mastitis database derived from a 2-yr data collection on a national cohort of dairy farms, and data from this initiative were used to further investigate the effect of IMI with minor pathogens on the acquisition of new major pathogen infections (defined as a culture-positive quarter sample in a quarter that had been free of that major pathogen in previous samples in the sampling period). Longitudinal milk samplings of clinically normal udders taken over several 6-wk periods as well as samples from cows pre-dry-off and postcalving were used to this end (n=80,397 quarter milk samples). The effects of CNS and Corynebacterium spp. on the major mastitis pathogens Staph. aureus, Strep. uberis, Strep. dysgalactiae, and coliform bacteria (Escherichia coli and Klebsiella spp.) were investigated using risk ratio analyses and multilevel logistic regression models. Quarter-, cow- and herd-level susceptibility parameters were also evaluated and were able to account for the increased susceptibility that exists within herds, cows and quarters, removing it from estimates for the effects of the minor pathogens. Increased quarter-level susceptibility was associated with increased risk of major pathogen NIMI for all pathogens except the coliforms. Increased somatic cell count was consistently associated with elevated risk of new major pathogen infections, but this was assumed to be a result of low sensitivity of bacteriology to diagnose major pathogen NIMI expediently and accurately. The presence of CNS in the sample 2 samplings before the occurrence of a NIMI increased the odds of experiencing a Staph. aureus NIMI 2.0 times, making the presence of CNS a risk factor for acquiring a Staph. aureus NIMI. Even with this extensive data set, power was insufficient to make a definitive statement about the effect of minor pathogen IMI on the acquisition of major pathogen NIMI. Definitively answering questions of this nature are likely to require an extremely large data set dedicated particularly to minor pathogen presence and NIMI with major pathogens.Source type: Electronic(1

EXTENDING THE INDUCTION PERIOD OF CRYSTALLIZATION FOULING THROUGH SURFACE COATING

To minimize the negative effects of scale formation in heat exchangers, new anti-fouling strategies are focusing on the modification of heat transfer surfaces. These modifications should lead to tailored made surfaces for different technical applications. Aim of this surface modification is the extension of the induction period to minimize the negative effects of fouling and maximize the endurance of the heat exchanger. To achieve such, different surface coatings on stainless steel were investigated in respect of fouling tendency. The effect of flow velocity respectively Reynolds number on the induction time of CaSO4 crystallization fouling were tested in different test units. Diamond like carbon coatings are extending the induction time in every measured flow velocity. At higher Reynolds numbers, the effect of different surface crystallization due to energetic modification is reduced because of the dominating effect of the low adhesive surface. Thus the induction time can be extended by the factor of 2 for low fluid velocities (DLC or SICON®) and more than 20 for higher Reynolds numbers (DLC and SICON®). The combination of limited nucleation spots due to electro-chemical treatment of the substrate before coating can be a tailored made surface with maximum induction time for crystallization fouling

Simple heuristics for the assembly line worker assignment and balancing problem

We propose simple heuristics for the assembly line worker assignment and balancing problem. This problem typically occurs in assembly lines in sheltered work centers for the disabled. Different from the classical simple assembly line balancing problem, the task execution times vary according to the assigned worker. We develop a constructive heuristic framework based on task and worker priority rules defining the order in which the tasks and workers should be assigned to the workstations. We present a number of such rules and compare their performance across three possible uses: as a stand-alone method, as an initial solution generator for meta-heuristics, and as a decoder for a hybrid genetic algorithm. Our results show that the heuristics are fast, they obtain good results as a stand-alone method and are efficient when used as a initial solution generator or as a solution decoder within more elaborate approaches.Comment: 18 pages, 1 figur

Measuring the Solar Radius from Space during the 2003 and 2006 Mercury Transits

The Michelson Doppler Imager (MDI) aboard the Solar and Heliospheric Observatory observed the transits of Mercury on 2003 May 7 and 2006 November 8. Contact times between Mercury and the solar limb have been used since the 17th century to derive the Sun's size but this is the first time that high-quality imagery from space, above the Earth's atmosphere, has been available. Unlike other measurements this technique is largely independent of optical distortion. The true solar radius is still a matter of debate in the literature as measured differences of several tenths of an arcsecond (i.e., about 500 km) are apparent. This is due mainly to systematic errors from different instruments and observers since the claimed uncertainties for a single instrument are typically an order of magnitude smaller. From the MDI transit data we find the solar radius to be 960".12 +/- 0".09 (696,342 +/- 65 km). This value is consistent between the transits and consistent between different MDI focus settings after accounting for systematic effects.Comment: Accepted for publication in The Astrophysical Journal (2012 March 5

Generation and characterization of a mitotane-resistant adrenocortical cell line

Mitotane is the only drug approved for the therapy of adrenocortical carcinoma (ACC). Its clinical use is limited by the occurrence of relapse during therapy. To investigate the underlying mechanisms in vitro, we here generated mitotane-resistant cell lines. After long-term pulsed treatment of HAC-15 human adrenocortical carcinoma cells with 70 µM mitotane, we isolated monoclonal cell populations of treated cells and controls and assessed their respective mitotane sensitivities by MTT assay. We performed exome sequencing and electron microscopy, conducted gene expression microarray analysis and determined intracellular lipid concentrations in the presence and absence of mitotane. Clonal cell lines established after pulsed treatment were resistant to mitotane (IC50 of 102.2 ± 7.3 µM (n = 12) vs 39.4 ± 6.2 µM (n = 6) in controls (biological replicates, mean ± s.d., P = 0.0001)). Unlike nonresistant clones, resistant clones maintained normal mitochondrial and nucleolar morphology during mitotane treatment. Resistant clones largely shared structural and single nucleotide variants, suggesting a common cell of origin. Resistance depended, in part, on extracellular lipoproteins and was associated with alterations in intracellular lipid homeostasis, including levels of free cholesterol, as well as decreased steroid production. By gene expression analysis, resistant cells showed profound alterations in pathways including steroid metabolism and transport, apoptosis, cell growth and Wnt signaling. These studies establish an in vitro model of mitotane resistance in ACC and point to underlying molecular mechanisms. They may enable future studies to overcome resistance in vitro and improve ACC treatment in vivo

Transient magnetoconductivity of photoexcited electrons

Transient magnetotransport of two-dimensional electrons with partially-inverted distribution excited by an ultrashort optical pulse is studied theoretically. The time-dependent photoconductivity is calculated for GaAs-based quantum wells by taking into account the relaxation of electron distribution caused by non-elastic electron-phonon interaction and the retardation of the response due to momentum relaxation and due to a finite capacitance of the sample. We predict large-amplitude transient oscillations of the current density and Hall field (Hall oscillations) with frequencies corresponding to magnetoplasmon range, which are initiated by the instability owing to the absolute negative conductivity effect.Comment: 21 pages, 6 fig

Hanle effect in the CN violet system with LTE modeling

Weak entangled magnetic fields with mixed polarity occupy the main part of the quiet Sun. The Zeeman effect diagnostics fails to measure such fields because of cancellation in circular polarization. However, the Hanle effect diagnostics, accessible through the second solar spectrum, provides us with a very sensitive tool for studying the distribution of weak magnetic fields on the Sun. Molecular lines are very strong and even dominate in some regions of the second solar spectrum. The CN $B {}^{2} \Sigma - X {}^{2} \Sigma$ system is one of the richest and most promising systems for molecular diagnostics and well suited for the application of the differential Hanle effect method. The aim is to interpret observations of the CN $B {}^{2} \Sigma - X {}^{2} \Sigma$ system using the Hanle effect and to obtain an estimation of the magnetic field strength. We assume that the CN molecular layer is situated above the region where the continuum radiation is formed and employ the single-scattering approximation. Together with the Hanle effect theory this provides us with a model that can diagnose turbulent magnetic fields. We have succeeded in fitting modeled CN lines in several regions of the second solar spectrum to observations and obtained a magnetic field strength in the range from 10--30 G in the upper solar photosphere depending on the considered lines.Comment: Accepted for publication in Astronomy and Astrophysic

Automatic detection and tracking of filaments for a solar feature database

A new method for the automatic detection and tracking of solar filaments is presented. The method addresses the problems facing existing catalogs, such as the one developed recently in the frame of the European Grid of Solar Observations (EGSO) project. In particular, it takes into account the structural and temporal evolution of filaments, differences in intensity as seen from one observation to the next, and the possibility of sudden disappearance followed by reappearance. In this study, the problem of tracking is solved by plotting all detected filaments during each solar rotation on a Carrington map and then by applying region growing techniques on those plots. Using this approach, the "fixed" positions of the envelopes in the Carrington system can be deduced. This is followed by a backward tracking of each filament by considering one full solar rotation. The resulting shifted Carrington map then enables one to follow any filament from one rotation to the next. Such maps should prove valuable for studies of the role of filaments in solar activity, notably coronal mass ejections (CMEs)