1 research outputs found
Inhibitory effect of tannic acid and its derivative (gallic acid) against cisplatin–induced thiobarbituric acid reactive substances (TBARS) production in rat kidney – in vitro
Increasing amounts of evidence suggests tannic acid (TA) and its derivative,
gallic acid (GA) has beneficial effects against nephrotoxicity. The aim of this
study was to investigate the effect of TA and GA (two structurally related
phenolic acids) against cisplatin-induced thiobarbituric acid reactive
substances (TBARS) production in rat kidney in vitro. The effect of both
phenolic acids on kidney TBARS level in the presence of cisplatin, an antineoplastic drug used in the treatment of many solid-tissue cancers which has
its chief side effect in nephrotoxicity, was assessed. Thereafter, the
antioxidant properties were also determined. Incubation of the kidney tissue
homogenate in the presence of cisplatin (1 mM) caused a significant
(P<0.05) increase in the TBARS level. However, both phenolic acids caused
a significant (P<0.05) decrease in the TBARS content of the kidney in a dose
dependent manner (0 – 12.5 µM). Nevertheless, TA had a significantly
higher (P<0.05) inhibitory effect on TBARS production than gallic acid
judging by their IC50 value. Furthermore, TA possesses stronger antioxidant
properties than GA as revealed by their scavenging, chelating and reducing
ability. The inhibition of cisplatin–induced thiobarbituric acid reactive
substances (TBARS) production in rat kidney by both TA and GA could be
due to their antioxidant properties and this provide further insight into the
mechanism of action for their nephroprotective properties from previous
reported experimental studies. However, the study revealed that hydrolysis of
TA reduces its potenc