Shape Changes of Self-Assembled Actin Bilayer Composite Membranes

We report the self-assembly of thin actin shells beneath the membranes of giant vesicles. Ion-carrier mediated influx of Mg2+ induces actin polymerization in the initially spherical vesicles. Buckling of the vesicles and the formation of blisters after thermally induced bilayer expansion is demonstrated. Bilayer flickering is dominated by tension generated by its coupling to the actin cortex. Quantitative flicker analysis suggests the bilayer and the actin cortex are separated by 0.4 \mum to 0.5 \mum due to undulation forces.Comment: pdf-file, has been accepted by PR

Performance of the LHC Pre-Injectors

The LHC pre-injector complex, comprising Linac 2, the PS Booster (PSB) and the PS, has undergone a major upgrade in order to meet the very stringent requirements of the LHC. Whereas bunches with the nominal spacing and transverse beam brightness were already available from the PS in 1999 [1], their length proved to be outside tolerance due to a debunching procedure plagued by microwave instabilities. An alternative scenario was then proposed, based on a series of bunch-splitting steps in the PS. The entire process has recently been implemented successfully, and beams whose longitudinal characteristics are safely inside LHC specifications are now routinely available. Variants of the method also enable bunch trains with gaps of different lengths to be generated. These are of interest for the study and possible cure of electron cloud effects in both the SPS and LHC. The paper summarizes the beam dynamics issues that had to be addressed to produce beams with all the requisite qualities for the LHC

Transverse Performance of the Proton Beam Delivered by the CERN PS Complex for the Future LHC

The performance of the CERN LHC will depend heavily on the high-brightness beam delivered by the injector chain. In 1999, after completion of the programme of hardware upgrades of the PS Complex, a major effort was devoted to producing a proton beam with the nominal transverse characteristics foreseen for LHC operation. This paper focuses on the different beam dynamics issues encountered during the setting up of such a beam, in the Linac2, the PS-Booster (PSB), the PS and the TT2 transfer line to the SPS. During the setting-up, single-particle issues, like stop-band compensation, correction of injection oscillations during the double-batch injection process, and the correction of the high-energy closed orbit in the PS, were addressed. Furthermore, collective effects, such as high-order head-tail instabilities induced by the resistive-wall impedance, were observed and cured. The compensation of these harmful phenomena permitted to achieve the goal, namely the generation of small transverse beam emittances and their conservation along the chain of different machines

Design of a 2.2 GeV Accumulator and Compressor for a Neutrino Factory

The proton driver for a neutrino factory must provide megawatts of beam power at a few GeV, with nonosecond long bunches each containing more than 1x1012 protons. Such beam powers are within reach of a high-energy linac, but the required time structure cannot be provided without accumulation and compression. The option of a linac-based 2.2 GeV proton driver has been studied at CERN, taking into account the space charge and stability problems which make beam accumulation and bunch compression difficult at such a low-energy. A solution featuring two rings of approximately 1 km circumference has been worked out and is described in this paper. The subjects deserving further investigation are outlined

Proton Drivers for Neutrino Factories: The CERN Approach

The paper describes the CERN approach for a proton driver for a Neutrino Factory. Two main layouts are presented: the so-called CERN Reference Scenario, based on a 2.2 GeV linac and an alternative one, based on a 30 GeV synchrotron. Both produce bunches of 1 ns (r.m.s.) and a beam power of 4 MW

Oxidative Stress-Induced STIM2 Cysteine Modifications Suppress Store-Operated Calcium Entry

Store-operated calcium entry (SOCE) through STIM-gated ORAI channels governs vital cellular functions. In this context, SOCE controls cellular redox signaling and is itself regulated by redox modifications. However, the molecular mechanisms underlying this calcium-redox interplay and the functional outcomes are not fully understood. Here, we examine the role of STIM2 in SOCE redox regulation. Redox proteomics identify cysteine 313 as the main redox sensor of STIM2 in vitro and in vivo. Oxidative stress suppresses SOCE and calcium currents in cells overexpressing STIM2 and ORAI1, an effect that is abolished by mutation of cysteine 313. FLIM and FRET microscopy, together with MD simulations, indicate that oxidative modifications of cysteine 313 alter STIM2 activation dynamics and thereby hinder STIM2-mediated gating of ORAI1. In summary, this study establishes STIM2-controlled redox regulation of SOCE as a mechanism that affects several calcium-regulated physiological processes, as well as stress-induced pathologies

Acceleration of lead ions in the CERN PS booster and the CERN PS

The new CERN Heavy Ion Accelerating Facility also requires besides a new Linac substantial modifications of existing accelerators. They are imposed by the low speed and the low intensity of the ion beam and, crucially at low energy, by the short lifetime of the partially stripped ions due to charge exchange with the atoms of the residual gas. The upgraded vacuum system hits the limits of a non-bakeable machine and consequently the acceleration had to be sped up by all means. In the Booster this led to injection and RF capture on a fast-rising magnet cycle and a new digital RF beam control system. Beam current transformers had to be replaced by new, heavily shielded ones. Other modifications include a new staircase magnet to distribute ions over the four Booster rings, lengthening of septa and kicker pulses, plus new, bakeable extraction septa and an energy stabilizing RF loop on the flat top in the CPS, and a stripper in the transfer line to the SPS

Id-1 stimulates cell proliferation through activation of EGFR in ovarian cancer cells

Increased EGFR (epidermal growth factor receptor) expression has been reported in many types of human cancer and its levels are positively associated with advanced cancers. Recently, upregulation of Id-1 (inhibitor of differentiation or DNA binding) protein was found in over 70% of ovarian cancer samples and correlated with poor survival of ovarian cancer patients. However, the molecular mechanisms responsible for the role of Id-1 in ovarian cancer are not clear. The aim of this study was to investigate the effect of Id-1 on ovarian cancer proliferation and its association with the EGFR pathway. To achieve this, we transfected an Id-1 expression vector into three ovarian cancer cell lines and examined cell proliferation rate by flow cytometry and bromodeoxyuridine staining. We found that ectopic Id-1 expression led to increased cell proliferation demonstrated by increased BrdU incorporation rate and S-phase fraction. The Id-1-induced cell growth was associated with upregulation of EGFR at both transcriptional and protein levels. In contrast, inactivation of Id-1 through transfection of an Id-1 antisense vector resulted in downregulation of EGFR. Our results indicate that increased Id-1 in ovarian cancer cells may promote cancer cell proliferation through upregulation of EGFR. Our findings also implicate that Id-1 may be a potential target for the development of novel strategies in the treatment of ovarian cancer. © 2004 Cancer Research UK.link_to_OA_fulltex