Respiratory electron transfer pathways in plant mitochondria

The respiratory electron transport chain (ETC) couples electron transfer from organic substrates onto molecular oxygen with proton translocation across the inner mitochondrial membrane. The resulting proton gradient is used by the ATP synthase complex for ATP formation. In plants, the ETC is especially intricate. Besides the "classical" oxidoreductase complexes (complex I-IV) and the mobile electron transporters cytochrome c and ubiguinone, it comprises numerous "alternative oxidoreductases." Furthermore, several dehydrogenases localized in the mitochondrial matrix and the mitochondrial intermembrane space directly or indirectly provide electrons for the ETC. Entry of electrons into the system occurs via numerous pathways which are dynamically regulated in response to the metabolic state of a plant cell as well as environmental factors. This mini review aims to summarize recent findings on respiratory electron transfer pathways in plants and on the involved components and supramolecular assemblies.DFG/BR/1829/10-

3-hydroxyisobutyrate dehydrogenase is involved in both, valine and isoleucine degradation in arabidopsis thaliana

In plants, amino acid catabolism is especially relevant in metabolic stress situations (e.g. limited carbohydrate availability during extended darkness). Under these conditions, amino acids are used as alternative substrates for respiration. Complete oxidation of the branched-chain amino acids (BCAAs) leucine, isoleucine (Ile), and valine (Val) in the mitochondria efficiently allows the formation of ATP by oxidative phosphorylation. However, the metabolic pathways for BCAA breakdown are largely unknown so far in plants. A systematic search for Arabidopsis (Arabidopsis thaliana) genes encoding proteins resembling enzymes involved in BCAA catabolism in animals, fungi, and bacteria as well as proteomic analyses of mitochondrial fractions from Arabidopsis allowed the identification of a putative 3-hydroxyisobutyrate dehydrogenase, AtHDH1 (At4g20930), involved in Val degradation. Systematic substrate screening analyses revealed that the protein uses 3-hydroxyisobutyrate but additionally 3-hydroxypropionate as substrates. This points to a role of the enzyme not only in Val but possibly also in Ile metabolism. At4g20930 knockdown plants were characterized to test this conclusion. Root toxicity assays revealed increased root growth inhibition of the mutants if cultivated in the presence of Val or Ile but not in the presence of leucine. We conclude that AtHDH1 has a dual role in BCAA metabolism in plants

Mitochondrial dehydrogenases in Arabidopsis thaliana

[no abstract

Properties of the close binary and circumbinary torus of the Red Rectangle

New diffraction-limited speckle images of the Red Rectangle in the wavelength range 2.1--3.3 microns with angular resolutions of 44--68 mas and previous speckle images at 0.7--2.2 microns revealed well-resolved bright bipolar outflow lobes and long X-shaped spikes originating deep inside the outflow cavities. This set of high-resolution images stimulated us to reanalyze all infrared observations of the Red Rectangle using our two-dimensional radiative transfer code. The new detailed modeling, together with estimates of the interstellar extinction in the direction of the Red Rectangle enabled us to more accurately determine one of the key parameters, the distance D=710 pc with model uncertainties of 70 pc, which is twice as far as the commonly used estimate of 330 pc. The central binary is surrounded by a compact, massive (M=1.2 Msun), very dense dusty torus with hydrogen densities reaching n_H=2.5x10^12 cm^-3 (dust-to-gas mass ratio rho_d/rho~0.01). The bright component of the spectroscopic binary HD 44179 is a post-AGB star with mass M*=0.57 Msun, luminosity L*=6000 Lsun, and effective temperature T*=7750 K. Based on the orbital elements of the binary, we identify its invisible component with a helium white dwarf with Mwd~0.35 Msun, Lwd~100 Lsun, and Twd~6x10^4 K. The hot white dwarf ionizes the low-density bipolar outflow cavities inside the dense torus, producing a small HII region observed at radio wavelengths. We propose an evolutionary scenario for the formation of the Red Rectangle nebula, in which the binary initially had 2.3 and 1.9 Msun components at a separation of 130 Rsun. The nebula was formed in the ejection of a common envelope after Roche lobe overflow by the present post-AGB star.Comment: 20 pages, 10 figures, accepted by Astronomy and Astrophysics, also available at http://www.mpifr-bonn.mpg.de/div/ir-interferometry/publications.htm

The extracellular matrix of hematopoietic stem cell niches

Hematopoietic stem cells (HSCs) are the life-long source of all types of blood cells. Their function is controlled by their direct microenvironment, the HSC niche in the bone marrow. Although the importance of the extracellular matrix (ECM) in the niche by orchestrating niche architecture and cellular function is widely acknowledged, it is still underexplored. In this review, we provide a comprehensive overview of the ECM in HSC niches. For this purpose, we first briefly outline HSC niche biology and then review the role of the different classes of ECM molecules in the niche one by one and how they are perceived by cells. Matrix remodeling and the emerging importance of biophysics in HSC niche function are discussed. Finally, the application of the current knowledge of ECM in the niche in form of artificial HSC niches for HSC expansion or targeted differentiation as well as drug testing is reviewed. © 2021 The Author(s

Mineral inclusions in rutile: A novel recorder of HP-UHP metamorphism

The ability to accurately constrain the secular record of high- and ultra-high pressure metamorphism on Earth is potentially hampered as these rocks are metastable and prone to retrogression, particularly during exhumation. Rutile is among the most widespread and best preserved minerals in high- and ultra-high pressure rocks and a hitherto untested approach is to use mineral inclusions within rutile to record such conditions. In this study, rutiles from three different high- and ultrahigh-pressure massifs have been investigated for inclusions. Rutile is shown to contain inclusions of high-pressure minerals such as omphacite, garnet and high silica phengite, as well as diagnostic ultrahigh-pressure minerals, including the first reported occurrence of exceptionally preserved monomineralic coesite in rutile from the Dora–Maira massif. Chemical comparison of inclusion and matrix phases show that inclusions generally represent peak metamorphic assemblages; although rare prograde phases such as titanite, omphacite and corundum have also been identified implying that rutile grows continuously during prograde burial and traps mineralogic evidence of this evolution. Pressure estimates obtained from mineral inclusions, when used in conjunction with Zr-in-rutile thermometry, can provide additional constraints on the metamorphic conditions of the host rock. This study demonstrates that rutile is an excellent repository for high- and ultra-high pressure minerals and that the study of mineral inclusions in rutile may profoundly change the way we investigate and recover evidence of such events in both detrital populations and partially retrogressed samples

L-galactono-1,4-lactone dehydrogenase (GLDH) forms part of three subcomplexes of mitochondrial complex I in Arabidopsis thaliana

L-Galactono-1,4-lactone dehydrogenase (GLDH) catalyzes the terminal step of the Smirnoff-Wheeler pathway for vitamin C (L-ascorbate) biosynthesis in plants. A GLDH in gel activity assay was developed to biochemically investigate GLDH localization in plant mitochondria. It previously has been shown that GLDH forms part of an 850-kDa complex that represents a minor form of the respiratory NADH dehydrogenase complex (complex I). Because accumulation of complex I is disturbed in the absence of GLDH, a role of this enzyme in complex I assembly has been proposed. Here we report that GLDH is associated with two further protein complexes. Using native gel electrophoresis procedures in combination with the in gel GLDH activity assay and immunoblotting, two mitochondrial complexes of 470 and 420 kDa were identified. Both complexes are of very low abundance. Protein identifications by mass spectrometry revealed that they include subunits of complex I. Finally, the 850-kDa complex was further investigated and shown to include the complete "peripheral arm" of complex I. GLDH is attached to a membrane domain, which represents a major fragment of the "membrane arm" of complex I. Taken together, our data further support a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates.Instituto de Fisiología Vegeta

L-galactono-1,4-lactone dehydrogenase (GLDH) forms part of three subcomplexes of mitochondrial complex I in Arabidopsis thaliana

L-Galactono-1,4-lactone dehydrogenase (GLDH) catalyzes the terminal step of the Smirnoff-Wheeler pathway for vitamin C (L-ascorbate) biosynthesis in plants. A GLDH in gel activity assay was developed to biochemically investigate GLDH localization in plant mitochondria. It previously has been shown that GLDH forms part of an 850-kDa complex that represents a minor form of the respiratory NADH dehydrogenase complex (complex I). Because accumulation of complex I is disturbed in the absence of GLDH, a role of this enzyme in complex I assembly has been proposed. Here we report that GLDH is associated with two further protein complexes. Using native gel electrophoresis procedures in combination with the in gel GLDH activity assay and immunoblotting, two mitochondrial complexes of 470 and 420 kDa were identified. Both complexes are of very low abundance. Protein identifications by mass spectrometry revealed that they include subunits of complex I. Finally, the 850-kDa complex was further investigated and shown to include the complete "peripheral arm" of complex I. GLDH is attached to a membrane domain, which represents a major fragment of the "membrane arm" of complex I. Taken together, our data further support a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates

Multiple Growth Mechanisms of Jadeite in Cuban Metabasite

Samples of rocks reported in the literature to be jadeite jade from the subduction-zone complex of the Escambray Massif in\ud central Cuba have been studied by optical and transmission electron microscopy, electron microprobe and hot-cathode cathodoluminescence\ud (CL) microscopy. Although these rocks are indeed rich in jadeite, the bulk rock composition generally conforms to\ud MORB, with Na2O enriched by . 3 wt% and CaO depleted by .2 wt%. Al2O3 contents are unchanged. These changes are attributed\ud to early pre-subduction spilitization of the ocean-floor protolith. Relics of magmatic augite preserving an ophitic texture are common.\ud Disequilibrium textures are the rule. Extensively recrystallized rocks show fine, felty intergrowths of predominantly Al-rich\ud glaucophane and jadeite, the latter with rims and patches of omphacite. TEM observations indicate extensive replacement of\ud pyroxene by amphibole. Glaucophane developed rims of magnesiokatophorite and edenite. Chlorite and epidote are also present.\ud Late development of actinolite, chlorite, epidote and albite is observed. Quartz is present. Less recrystallized samples with numerous\ud large (.1.5 mm) grains of augite show several types of sodic and sodic-calcic clinopyroxene development: (1) Topotactic\ud replacement of magmatic pyroxene by jadeite and omphacite along a broad front encroaching upon the augite grain from the rock\ud matrix. Jadeite dominates where presumably plagioclase was formerly present. Omphacite dominates where augite is internally\ud replaced along cleavage and fractures. Late chlorite, taramite and ferropargasite replace these pseudomorphs. (2) Former plagioclase\ud laths of the ophitic fabric are replaced by jadeite together with lesser omphacite in epitactic relationship with the enclosing augite.\ud Former plagioclase-augite grain boundaries remain preserved. Late pumpellyite is associated with the omphacite. (3) Jadeite þ\ud omphacite þ pumpellyite þ chlorite with irregular grain boundaries dominate in the rock matrix between the augite relics, with\ud idiomorphic crystals of epidote scattered throughout and in chlorite–epidote clusters. Pumpellyite is interpreted to be a late retrograde\ud product. Quartz is present. (4) Jadeite þ omphacite þ chlorite assemblages, in which monomineralic sheaf-like jadeite aggregates are\ud common, fill very thin (500–1500 mm) fractures criss-crossing the sample, including ophitic augite remnants. Cathodoluminescence\ud microscopy shows that jadeite in the veins is distinctly different from CL in the other types of jadeite, showing features like oscillatory\ud growth zoning indicative of crystallization from a fluid. Generally omphacite develops irregularly along jadeite rims, but recrystallization\ud may lead to pairs with straight grain boundaries suggestive of phase equilibration. Comparison with published solvus\ud relationships suggests temperatures of 425–500 C. This unusual occurrence of different types of jadeite in a metabasic rock suggests\ud two contrasting sources. The first – in the rock matrix, as topotactic alteration of igneous pyroxene and as plagioclase replacement\ud epitactically growing on augite – can be explained as due to local domain equilibration in a rapidly subducted ‘‘spilitized’’ gabbroic\ud rock. The second, in very thin fracture fillings, conforms to an origin as a crystallization product from a pervasive fluid. Conceivably,\ud ‘‘pooling’’ of the fluids flowing through the fractures in larger cavities could lead to larger masses of jadeitite. These have not yet been\ud conclusively documented in the Escambray Massif