Expérience et compétences des guichetiers de la Poste dans leurs rapports à la règle

International audienceIl n’y a pas si longtemps on s’intéressait guère aux activités de service en ergonomie. Aujourd’hui la recherche développe des études sur le terrain afin de tenter de construire un modèle pope à ce type d’activité. A partir d’une question sur la genèse de l’inaptitude à la Poste nous avons entrepris une démarche de compréhension sur la construction des compétences et de l’expérience comme une stratégie de prévention de l’inaptitude. Nos premières observations nous indiquent l’importance du rapport à la règle et notamment de ces transgressions comme un moyen de régulation. Au delà de cette stratégie individuelle, l’absence de collectif de travail apparaît dans le bureau de Poste étudié comme une source de la désadaptation

Policy implications of the papers in the special issue of WORK concerning Work and family, produced by the IEA Gender and Work Technical Committee

International audienc

A rational blueprint for the design of chemically-controlled protein switches

Small-molecule responsive protein switches are crucial components to control synthetic cellular activities. However, the repertoire of small-molecule protein switches is insufficient for many applications, including those in the translational spaces, where properties such as safety, immunogenicity, drug half-life, and drug side-effects are critical. Here, we present a computational protein design strategy to repurpose drug-inhibited protein-protein interactions as OFF- and ON-switches. The designed binders and drug-receptors form chemically-disruptable heterodimers (CDH) which dissociate in the presence of small molecules. To design ON-switches, we converted the CDHs into a multi-domain architecture which we refer to as activation by inhibitor release switches (AIR) that incorporate a rationally designed drug-insensitive receptor protein. CDHs and AIRs showed excellent performance as drug responsive switches to control combinations of synthetic circuits in mammalian cells. This approach effectively expands the chemical space and logic responses in living cells and provides a blueprint to develop new ON- and OFF-switches.ISSN:2041-172

Bottom-up de novo design of functional proteins with complex structural features

Data underlying the figures in the publication “Bottom-up de novo design of functional proteins with complex structural features” in Nat Chem Biol, 2021, (https://doi.org/10.1038/s41589-020-00699-x). Table of contents: 1. Figure 3b; CD Spectra 2. Figure 3c; SPR Spectra 3. Figure 5b-g; Antibody Biosensors Response Study 4. Figure 6; Activity Study 5. Extended Data Figure 7; Thermal Denaturation Curve 6. Extended Data Figure 8; Comparative Luminescence Spectr