Phytoplankton productivity in newly dug fish ponds within Lake Victoria wetlands (Uganda)

The declining Lake Victoria fisheries resource led to a growing recognition of aquaculture as a source of livelihood to riparian communities. Finger ponds speculated to naturally stock fish during flooding and retain them during dry seasons were introduced within the lake’s wetlands. In order to develop a better understanding of these ponds’ dynamics, algal primary productivity was studied in eight newly dug wetland fishponds (8 x 24 m) located in two villages along the northern shores of Lake Victoria (Uganda) before stocking them with fish. Gross primary productivity was low for both sites ranging between 0.00 and 2.63 mg O2 L-1 h-1. The net areal primary productivity of Gaba ponds ranged from -0.34 to 4.66 mg O2 m-2 d-1 while that of Walukuba ponds ranged from 1.16 to 6.25 mg O2 m-2 d-1. Chlorophyll a mean values were 23.46 ± 12.50 ìg L-1 and 75.56 ± 44.35 ìg L-1 and mean turbidity ranges were 132.1 –242.25 and 432.54 - 158.49 NTU for Gaba and Walukuba ponds respectively. Reduced light supply due to the high inorganic turbidity may have been the main limitation for photoautotrophic primary productivity and ponds potential fish yield of 10 - 24 kg ha-1 fish per year

The effect of ply folds as manufacturing defect on the fatigue life of CFRP materials

Manufacturing defects are inherent to any manufacturing process. However, in composite materials they might be unavoidable, e.g. ply waviness or even folds of plies are present in complex shaped parts during high pressure resin transfer molding of carbon fiber reinforced polymers. In this work, the effect of the ply folds on the fatigue life of the composite material is investigated. Folds along fiber direction (as they commonly appear during manufacturing) were artificially introduced in unidirectional non crimp fabric plies. The target of this study is the prediction of damage initiation due to this particular type of manufacturing defect. The folds locally increase the fiber volume fraction and also introduce resin rich areas. Fatigue tests in fiber direction and transverse to fiber direction are performed at different load ratios under constant amplitude loading. The influence of the defect geometry on damage initiation and progression is investigated at different scales by non-destructive methods before testing, continuous strain measurement and monitoring the damage progression during testing and fractography analysis after final failure. Most of the time, the first damage was observed at the location of the introduced fold for all considered load cases. However, it was also found, that the folds lead to no significant reduction in fatigue life.&nbsp

Antagonistic interactions between filamentous heterotrophs and the cyanobacterium Nostoc muscorum

Background: Little is known about interactions between filamentous heterotrophs and filamentous cyanobacteria. Here, interactions between the filamentous heterotrophic bacteria Fibrella aestuarina (strain BUZ 2) and Fibrisoma limi (BUZ 3) with an axenic strain of the autotrophic filamentous cyanobacterium Nostoc muscorum (SAG 25.82) were studied in mixed cultures under nutrient rich (carbon source present in medium) and poor (carbon source absent in medium) conditions. Findings: F. aestuarina BUZ 2 significantly reduced the cyanobacterial population whereas F. limi BUZ 3 did not. Physical contact between heterotrophs and autotroph was observed and the cyanobacterial cells showed some level of damage and lysis. Therefore, either contact lysis or entrapment with production of extracellular compounds in close vicinity of host cells could be considered as potential modes of action. The supernatants from pure heterotrophic cultures did not have an effect on Nostoc cultures. However, supernatant from mixed cultures of BUZ 2 and Nostoc had a negative effect on cyanobacterial growth, indicating that the lytic compounds were only produced in the presence of Nostoc. The growth and survival of tested heterotrophs was enhanced by the presence of Nostoc or its metabolites, suggesting that the heterotrophs could utilize the autotrophs and its products as a nutrient source. However, the autotroph could withstand and out-compete the heterotrophs under nutrient poor conditions. Conclusions: Our results suggest that the nutrients in cultivation media, which boost or reduce the number of heterotrophs, were the important factor influencing the outcome of the interplay between filamentous heterotrophs and autotrophs. For better understanding of these interactions, additional research is needed. In particular, it is necessary to elucidate the mode of action for lysis by heterotrophs, and the possible defense mechanisms of the autotrophs

Vertical Distribution of Epibenthic Freshwater Cyanobacterial Synechococcus spp. Strains Depends on Their Ability for Photoprotection

Epibenthic cyanobacteria often grow in environments where the fluctuation of light intensity and quality is extreme and frequent. Different strategies have been developed to cope with this problem depending on the distribution of cyanobacteria in the water column. and either constant or enhanced levels of carotenoids were assayed in phycocyanin-rich strains collected from 1.0 and 0.5 m water depths. Protein analysis revealed that while the amount of biliproteins remained constant in all strains during light stress and recovery, the amount of D1 protein from photosystem II reaction centre was strongly reduced under light stress conditions in strains from 7.0 m and 1.0 m water depth, but not in strains collected from 0.5 m depth. spp. strains, depending on their genetically fixed mechanisms for photoprotection

Patterns of major photosynthetic pigments in freshwater algae. 1. Cyanoprokaryota, Rhodophyta and Cryptophyta

This study investigated major pigment patterns of 8 cyanoprokaryota, 2 rhodophytes and 2 cryptomonads isolated from freshwater ecosystems. Analysis was done by means of HPLC. The method, historically adapted to marine phytoplankton, was modified to accommodate limnic algae. Quantitative results obtained in this study can be used for phytoplankton quantification techniques based on pigment patterns. Compared to marine strains, the studied freshwater cyanoprokaryote strains reveal a more complex pigment pattern, including myxoxanthophyll, canthaxanthin and echinenone. Cryptophyta possess the two acetylenic class-specific marker compounds allo- and monadoxanthin, crocoxanthin was not detectable. Rhodophytes show a simple pigment pattern similar to marine species. Previous reports as to the existence of chlorophyll-d could not be confirmed (historical reports probably refer to an artefact of preparation). Besides methodological considerations, the phenomenon of complementary chromatic adaptation is discussed briefly

Patterns of major photosynthetic pigments in freshwater algae. 2. Dinophyta, Euglenophyta, Chlorophyceae and Charales

Major pigment patterns of 18 chlorophyceae, 7 charales, 4 euglenophyta, and 2 dinophyta isolated from freshwater ecosystems, were investigated by means of HPLC. In this study, quantitative results are presented, too, which are capable for phytoplankton quantification techniques based on pigment patterns. Chlorophyceae revealed a pattern similar to that of higher plants, but in some strains, loroxanthin as well as α-carotene were present. In charophytes, except for vegetative specimens of Chara tomentosa, γ-carotene was detected in antheridia only. Among investigated freshwater euglenophytes diadinoxanthin was the major carotenoid, with neoxanthin and ß-carotene present in minor amounts. Besides chlorophylls-a and -c, dinophytes contained high quantities of peridinin, whereas fucoxanthin was absent. An unknown component eluting just before violaxanthin showed a spectrum reminding of peridinin