Research of the power plant operational states with block structure

In this article the research technique block structure power plant operational states is offered. As an example the operating power plant of OOO Siberian Generation Company with block structure of turbogenerators connection is considered. The choice of the operating power plant has allowed to receive to carry out the analysis real long and emergency states. The offered technique of states identification and the analysis can be used for power plant of other structure after the corresponding correction

Исследование особенностей переноса радона в почвенном воздухе

Объектом исследования являются данные мониторинга плотности потока ионизирующего излучения в городе Томске на экспериментальной площадке Томской обсерватории радиоактивности и ионизирующих излучений. Цель работы – исследование особенностей переноса радона в почвенном воздухе. В процессе исследования проводился поиск закономерностей в поведении почвенного радона на суточном, годовом и синоптическом масштабах. В результате исследования выявлены факторы, влияющие на динамику радона в почве, а также сформированы базы данных по результатам измерения объемной активности радона на разных глубинах.The object of research is the data of ionizing radiation flux density monitoring on the site by Tomsk Observatory of radioactivity and ionizing radiation. Purpose is research of radon transfer in soil air. The study was carried out search of patterns in the soil radon behavior on the daily, annual and synoptic scale. The study identified factors affecting on soil radon dynamics, as well as a database formed by measuring radon concentration at different depths

Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks

Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long-term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colonyforming unit (CFU-f) frequency and the differentiation potential of MSC declined significantly. Relevant chromosomal aberrations were not detected by karyotyping and SNP-microarrays. Subsequently, we have compared DNA-methylation profiles with the Infinium HumanMethylation27 Bead Array and the profiles differed markedly in MSC derived from adipose tissue and bone marrow. Notably, all MSC revealed highly consistent senescence-associated modifications at specific CpG sites. These DNA-methylation changes correlated with histone marks of previously published data sets, such as trimethylation of H3K9, H3K27 and EZH2 targets. Taken together, culture expansion of MSC has profound functional implications - these are hardly reflected by genomic instability but they are associated with highly reproducible DNA-methylation changes which correlate with repressive histone marks. Therefore replicative senescence seems to be epigenetically controlled

Устройство синхронизации лазерного монитора

В процессе исследования проводились обзор литературы, поддержка оборудования.В результате исследования проводилисьпрактическая часть и результаты исследования устройство синхронизации лазерного монитора.In the process of the study, literature review, equipment support was conducted. The study resulted in the practical part and the results of the investigation of the laser monitor synchronization device

Технологическая подготовка производства изготовления детали "Вал выходящий" на станках с ЧПУ

В результате работы был проведен конструкторский анализ детали, разработан технологический процесс, выбрано оборудование для производства детали, составлены управляющие программы для станков с ЧПУ и разработаны карты наладки к ним. Рассчитаны припуски и допуски на технологические размеры,режимы резания и нормы времени. Предложены пути решения вопроса об экологической безопасности. Рассчитана экономическая эффективность.As a result of the work, a design analysis of the part was carried out, a technological process was developed, equipment for the production of parts was selected, control programs for CNC machine tools were compiled, and adjustment maps were developed for them. The allowances and tolerances for technological dimensions, cutting modes and time norms are calculated. Suggested ways to address the issue of environmental safety. Economic efficiency has been calculated

Proinflammatory phenotype of iPS cell-derived JAK2 V617F megakaryocytes induces fibrosis in 3D in vitro bone marrow niche

The myeloproliferative disease polycythemia vera (PV) driven by the JAK2 V617F mutation can transform into myelofibrosis (post-PV-MF). It remains an open question how JAK2 V617F in hematopoietic stem cells induces MF. Megakaryocytes are major players in murine PV models but are difficult to study in the human setting. We generated induced pluripotent stem cells (iPSCs) from JAK2 V617F PV patients and differentiated them into megakaryocytes. In differentiation assays, JAK2 V617F iPSCs recapitulated the pathognomonic skewed megakaryocytic and erythroid differentiation. JAK2 V617F iPSCs had a TPO-independent and increased propensity to differentiate into megakaryocytes. RNA sequencing of JAK2 V617F iPSC-derived megakaryocytes reflected a proinflammatory, profibrotic phenotype and decreased ribosome biogenesis. In three-dimensional (3D) coculture, JAK2 V617F megakaryocytes induced a profibrotic phenotype through direct cell contact, which was reversed by the JAK2 inhibitor ruxolitinib. The 3D coculture system opens the perspective for further disease modeling and drug discovery.</p