Assessment of serum metallograms in patients with purine urolithiasis before and after treatment

Investigations on the homeostasis of the hydroelectrolytic metabolism is important in the assessment of the uroconcrements’ biogenesis and of the therapeutic effects (based on diverse procedures). Our investigations have been performed on patients with purine urolithiasis included in two groups, according to the applied treatment for the removal of the uroconcrements. A group included 23 patients who underwent surgical treatment and the other group included 18 patients treated with Extracorporeal Shock-Wave Lithotripsy (ESWL). Before and after the therapeutic procedure the serum metallograms of the main alkaline (Na, K) and alkaline-earth (Ca, Mg) metals were determined by using spectrophotometry. The obtained results revealed post-treatment changes in the serum metallogram as follows: increase of natriemia and calcemia; decrease of kaliemia and magnesiemia in both groups. The find data show the dyshomeostasis of the main alkaline and alkaline-earth metals after the treatment underlying their implication in urolithogenesis

Xenobiochemic specificity of the deoxyribonucleic acid interaction with some cytostatic chemotherapeutics

The mostly recommended methods in oncotherapy are the surgical intervention, chemotherapy, radiotherapy, immunotherapy or a combination between them. The chemotherapy consists in the use of various drugs among which the most important are : the alkylating agents, antimetabolites, steroid hormones, antibiotics, phyto alkaloids, metal based drugs. In this review there are discussed the molecular mechanisms of the interaction of an alkylating cytostatic, i.e. cyclophosphamide (2 bis( -chloroethyl) amino-1-oxa-3-aza-2-phosphocyclohexan-2-oxide) – Cp and of a metal based cytostatic, i.e. cis-platinumum (cis-dichlorodiammineplatinum) - cDDP with deoxyribonucleic acid (DNA). Cyclophosphamide and cis-platinum present similar mechanism of interaction with DNA. Interacting with DNA these cytostatics give rise to mono- and bidentate adducts. The topics are of interest for comparative medicine (veterinary and human medicine). A particular importance arising from the appearance of the DNA-chemotherapeutic adducts is that these compounds can be detected analytically and can provide information on the consequences of the biochemical injury. Detection of DNA adducts is useful not only for the diagnosis of neoplastic diseases, but also for biomonitoring the evolution during chemotherapy