Hot Air Drying of Green Table Olives

The characteristics of hot air-drying of green table olives (Domat variety) by using a tray dryer were studied. Air temperature varied from 40 to 70 °C with an air velocity of 1 m/s. Drying rate curves were determined and quality of dried green olives was evaluated by instrumental analysis (bulk density, particle density, porosity, shrinkage, moisture content, water activity, colour value, protein content, oil content, peroxide value and acidity). Consumers’ acceptance test and microbiological analysis were also applied

Hot Air Drying of Green Table Olives

The characteristics of hot air-drying of green table olives (Domat variety) by using a tray dryer were studied. Air temperature varied from 40 to 70 °C with an air velocity of 1 m/s. Drying rate curves were determined and quality of dried green olives was evaluated by instrumental analysis (bulk density, particle density, porosity, shrinkage, moisture content, water activity, colour value, protein content, oil content, peroxide value and acidity). Consumers’ acceptance test and microbiological analysis were also applied

Production of laccase from Trametes versicolor by solid-state fermentation using olive leaves as a phenolic substrate

WOS: 000314269900009PubMed ID: 22763778The aim of the present study was to investigate whether olive leaves were feasible as a substrate for laccase production by the white-rot fungus Trametes versicolor FPRL 28A INI under solid-state fermentation conditions. Different experiments were conducted to select the variables that allow obtaining high levels of laccase activity. In particular, the effects of the initial moisture content, substrate particle size, supplementation with inorganic and organic nitrogen sources were evaluated. Highest laccase activity (276.62 +/- A 25.67 U/g dry substrate) was achieved with 80 % initial moisture content and 1.4-1.6 mm particle size of the substrate supplemented with yeast extract (1 % (w/w) nitrogen). Such a high activity was obtained without any addition of inducers.Ege University Research Fund (Izmir, Turkey)Ege University [009 MUH 008]This work was supported by Ege University Research Fund (Izmir, Turkey, Project no: 009 MUH 008). The authors wish to thank Dr. Tajalli Keshavarz and Christine S. Evans (Westminster University, England) for kindly supplying the fungal strain

Bioprocess strategies for production of xylanase on agro-residual products with Aureobasidium pullulans

WOS: 00034729860014

UTILIZATION OF RESIDUAL BEER FOR RED PIGMENT PRODUCTION BY MONASCUS PURPUREUS IN SUBMERGED FERMENTATION

Goksungur, Yekta/0000-0002-5166-6561WOS: 000514253400040This paper describes the production of red pigment by Monascus purpureus CMU001 in submerged fermentation system using residual beer as an inexpensive substrate. Alcohol and CO2 removed residual beer supplemented with 7.5 g/l of monosodium glutamate was used as the fermentation medium. Shaking speed and medium volume was found to affect red pigment synthesis by M.purpureus. Maximum red pigment production of 18.54 UA(510nm) was obtained with 200 rpm shaking speed, 50 ml of the fermentation medium, initial pH of 7.0, inoculation ratio of 2% (v/v). Several nitrogen sources were screened, and the highest pigment synthesis was obtained with monosodium glutamate. Promising results were obtained with corn steep liquor and yeast extract when used as the nitrogen source in pigment production. the optimum monosodium glutamate concentration was found to be 7.5 g/L. Kinetics of pigment and biomass formation was determined under the optimized fermentation conditions over a fermentation period of 8 days. This is the first article that evaluates residual beer for pigment synthesis using M.purpureus in the submerged fermentation system.Ege University Scientific Research Project FundEge University [FYL-2018-20061]This research was supported by Ege University Scientific Research Project Fund (Project No: FYL-2018-20061). the researchers are thankful to Professor Saisamorn Lumyong for providing the Monascus purpureus CMU 001

Application of beta-glucuronidase-immobilised silica gel formulation to microfluidic platform for biotransformation of beta-glucuronides

WOS: 000430175100004PubMed ID: 29497885To improve the efficiency of reactions of beta-glucuronidase (GUS)-assisted glucuronic acid (GluA) removal within a microfluidic system. beta-glucuronidase from Helix pomatia was immobilised and characterised in silica-based sol-gel monoliths. Efficiency of the GUS-doped silica monoliths was tested for hydrolysis of p-Nitrophenyl-beta-d-glucuronide (pNP-GluA) in both ml-scaled medium via batch reactions and microfluidic environment via continuous-flow reactions. In the microfluidic platform, within a duration of 150 min of continuous operation (flow rate: 1 A mu L/min), the obtained highest pNP yield was almost 50% higher than that of the corresponding batchwise reaction. However, increased flow rates (3, 5, and 10 A mu L/min) resulted in lower conversion yields compared to 1 A mu L/min. The microfluidic platform demonstrated continuous hydrolytic activity for 7 days with considerable reaction yields while using a small amount of the enzyme. These results revealed that usage of the microreactors has considerable potential to efficiently obtain bioactive GluA-free aglycons from various plant-derived beta-glucuronides for pharmaceutical applications. [GRAPHICS] .Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113M050]The financial support provided by the Scientific and Technological Research Council of Turkey (TUBITAK, 113M050) is highly appreciated. Special thanks are offered to Dr Barbaros Cetin from the Bilkent University Microfluidics & Lab-on-a-chip Research Group for microfabrication studies, and Dr Bogdan Parakhonskiy from the Ghent University for his valuable comments on the manuscript

Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods

WOS: 000321227800002In this study, Trichoderma harzianum EGE-K38 was used for micropropagule production in solid-state fermentation. Various inexpensive agricultural co-products including wheat bran, sawdust, rice straw, hazelnut shell, grape marc, and cotton seed cake were tested as natural substrates, and zeolite was tested as an inert support for micropropagule production. Several parameters were determined to obtain maximum micropropagule production: the effects of various nitrogen sources, initial moisture content, initial pH, incubation temperature, and incubation time. Maximum micropropagule count achieved was (1.30 +/- 0.68) x 10(10) cfu/g dry substrate with a wheat bran malt sprout mixture (3:2), at 28 degrees C, an initial moisture content of 70% (w/w), and an initial pH of 5.8 +/- 0.3 after 4 days of cultivation. In zeolite-based medium, a maximum micropropagule count of (5.12 +/- 0.61) x 10(8) cfu/g dry inert support was achieved. Optimized parameters were used in a higher scale where micropropagules were produced in trays using a wheat bran malt sprout medium and harvested to determine the drying conditions in both lyophilization and oven-drying. It was found that up to 97% of micropropagules retained their viability with glycerol as a protectant after 1 day of oven-drying at 40 degrees C