Effect of 17- alpha- methyl testosterone on cichlid kaliko (Labeotropheous foellobroni) sex reversal

In this present study, Cichlid Kaliko (Labeotropheous foellobroni) fallowing was exposed to 17-alpha-methyl testosterone at 2 stages of development including prior to maturity (larvae) and after puberty (broodstock). The influence of sex hormone on the larvae was also explored. The first group was the larvae under the four treatments (0, 50,100, 250) mg per kg of food and hormones that broodstock group also included four treatments (0, 100, 250, 400) mg per kg food three replicates were performed in a period of 30 days. Hormone was added to fish food. Results showed that treatments had a significant difference in fish growth, which correlated with increased amounts of 17 alpha-methyl testosterone. In group I, food with 250mg/kg testerone resulted in an increase in growth indices (i.e.s, SGR=1.4, ADG= 6.5) with a mortality rate of 48.3 percent. In group II results showed that treatment of G (400mg testosterone per kg food) produced larvae with maximum growth indices (SGR=0.91, ADG= 3.6) and the least mortality rate of 31 percent; Research revealed that 17 alpha methyl testosterone could have an effect on Calico fish sex changes in both larval and stock stages

Knowledge mobilization activities to support decision-making by youth, parents, and adults using a systematic and living map of evidence and recommendations on COVID-19: protocol for three randomized controlled trials and qualitative user-experience studies

Abstract Introduction The COVID-19 pandemic underlined that guidelines and recommendations must be made more accessible and more understandable to the general public to improve health outcomes. The objective of this study is to evaluate, quantify, and compare the public’s understanding, usability, satisfaction, intention to implement, and preference for different ways of presenting COVID-19 health recommendations derived from the COVID-19 Living Map of Recommendations and Gateway to Contextualization (RecMap). Methods and analysis This is a protocol for a multi-method study. Through an online survey, we will conduct pragmatic allocation-concealed, blinded superiority randomized controlled trials (RCTs) in three populations to test alternative formats of presenting health recommendations: adults, parents, and youth, with at least 240 participants in each population. Prior to initiating the RCT, our interventions will have been refined with relevant stakeholder input. The intervention arm will receive a plain language recommendation (PLR) format while the control arm will receive the corresponding original recommendation format as originally published by the guideline organizations (standard language version). Our primary outcome is understanding, and our secondary outcomes are accessibility and usability, satisfaction, intended behavior, and preference for the recommendation formats. Each population’s results will be analyzed separately. However, we are planning a meta-analysis of the results across populations. At the end of each survey, participants will be invited to participate in an optional one-on-one, virtual semi-structured interview to explore their user experience. All interviews will be transcribed and analyzed using the principles of thematic analysis and a hybrid inductive and deductive approach. Ethics and dissemination Through Clinical Trials Ontario, the Hamilton Integrated Research Ethics Board has reviewed and approved this protocol (Project ID: 3856). The University of Alberta has approved the parent portion of the trial (Project ID:00114894). Findings from this study will be disseminated through open-access publications in peer-reviewed journals and using social media. Trial registration Clinicaltrials.gov NCT05358990 . Registered on May 3, 202