172 research outputs found
To the microscopic theory of the superconductive phase in antiferromagnetic metal compounds
The theory of the conductivity in antiferromagnetic metal compounds is constructed on the basic of the fluctuational theory of the magnetic superconductors. The superconductor in which there exist localized magnetic moments was considered. It is supposed that magnetic moments are orientated antiferromagnetically in basis plane of the crystal. An estimation for the critical temperature was obtained and necessary and enough conditions of the appearance of high-temperature superconductive phase in rare earth metal compounds were got. The criterion of an appearance of the high-temperature superconductive phase in antiferromagnetic compounds is found
EFFECTS OF CYP2D6 GENETIC POLYMORPHISMS ON THE EFFICACY AND SAFETY OF FLUVOXAMINE IN PATIENTS WITH DEPRESSIVE DISORDER AND COMORBID ALCOHOL USE DISORDER
Enzymatic biotransformation of adipic acid to 6-aminocaproic acid and 1,6- hexamethylenediamine using engineered carboxylic acid reductases and aminotransferases
Biocatalytic reduction of carboxylic acids is gaining importance for the production of polymer precursors and different chemicals. Carboxylic acid reductases (CARs) reduce carboxylic acids to aldehydes using ATP and NADPH as cofactors under mild conditions. Recently, we demonstrated that several bacterial CARs can reduce a broad range of bifunctional carboxylic acids containing amino group or second carboxylic group including adipic acid, which is a precursor for nylon-6-6 (Khusnutdinova et al., 2017). In this project, we demonstrate application of CARs and aminotransferases for further bioconversion of adipic acid to 6-aminocaproic acid and hexamethylenediamine, two other important precursors for nylon synthesis. Based on the crystal structure of the adenylating domain of the CAR enzyme MCH22995 from Mycobacterium chelonae, we generated a structural model of the CAR enzyme MAB4714 from M. abscessus, which is active toward adipic acid. Aiming at improving MAB4714 activity toward 6-aminocaproic acid, we used structure-based protein engineering and generated 16 MAB4714 mutant proteins. Screening of 16 purified MAB4714 variants against 6-aminocaproic acid,identified one protein, which was 10 times more active than the wild-type protein. We also identified several bacterial aminotransferases producing 6-aminocaproic acid from adipic acid in combination with CARs. Further optimization of reaction conditions and application of cofactor regeneration systems resulted in efficient biotransformation of adipic acid to 6-aminocaproic acid (88% conversion) and further to 1,6-hexamethylenediamine (78% conversion).
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Enzymatic Relay Mechanism Stimulates Cyclic GMP Synthesis in Rod Photoresponse: Biochemical and Physiological Study in Guanylyl Cyclase Activating Protein 1 Knockout Mice
Regulation of cGMP synthesis by retinal membrane guanylyl cyclase isozymes (RetGC1 and RetGC2) in rod and cone photoreceptors by calcium-sensitive guanylyl cyclase activating proteins (GCAP1 and GCAP2) is one of the key molecular mechanisms affecting the response to light and is involved in congenital retinal diseases. The objective of this study was to identify the physiological sequence of events underlying RetGC activation in vivo, by studying the electrophysiological and biochemical properties of mouse rods in a new genetic model lacking GCAP1. The GCAP1−/− retinas expressed normal levels of RetGC isozymes and other phototransduction proteins, with the exception of GCAP2, whose expression was elevated in a compensatory fashion. RetGC activity in GCAP1−/− retinas became more sensitive to Ca2+ and slightly increased. The bright flash response in electroretinogram (ERG) recordings recovered quickly in GCAP1−/−, as well as in RetGC1−/−GCAP1−/−, and RetGC2−/−GCAP1−/− hybrid rods, indicating that GCAP2 activates both RetGC isozymes in vivo. Individual GCAP1−/− rod responses varied in size and shape, likely reflecting variable endogenous GCAP2 levels between different cells, but single-photon response (SPR) amplitude and time-to-peak were typically increased, while recovery kinetics remained faster than in wild type. Recovery from bright flashes in GCAP1−/− was prominently biphasic, because rare, aberrant SPRs producing the slower tail component were magnified. These data provide strong physiological evidence that rod photoresponse recovery is shaped by the sequential recruitment of RetGC isozyme activation by GCAPs according to the different GCAP sensitivities for Ca2+ and specificities toward RetGC isozymes. GCAP1 is the ‘first-response’ sensor protein that stimulates RetGC1 early in the response and thus limits the SPR amplitude, followed by activation of GCAP2 that adds stimulation of both RetGC1 and RetGC2 to speed-up photoreceptor recovery
Ecosistema de información relacionado con el monitoreo del estilo de vida saludable de los adultos
This article discusses the problem of providing adults with reliable, relevant and timely information that allows them to organize their activities: the regime of work and rest in accordance with the goals of a healthy lifestyle and health correction. The goal is to determine the criteria and components of an information resource that will be adequate to the goals of keeping health of adults. The leading approaches to the health care mass media research are: structural-analytical, personality-oriented and cultural approaches. Research methods: analysis and synthesis of scientific and methodological literature and the information environment on the problems of the population health improving and methods of mathematical data processing. The article presents the results of determining the criteria for monitoring the information ecosystem on a healthy lifestyle, among these criteria the following were identified: the scientific validity of information, targeting, social significance and dialogue. Also, data portals that contribute to the solution of certain tasks of the adult population health improving were identified. The authors designed and implemented the informational and educational portal “3 youth” which is aimed at the integrated implementation of the basic health-improving needs of the adult population, taking into account the interconnection of health components: physical, mental, social. The results proving the effectiveness of using the authors' portal to ensure a healthy lifestyle for adults are presented and discussed. The article will be useful to adults who want to lead a healthy lifestyle with the support of the information resources, teachers and trainers working with people of mature age. This article is also useful for teachers of the Faculty of Physical Education who plan to promote a healthy lifestyle among different age categories.Este artículo analiza el problema de proporcionar a los adultos información confiable, relevante y oportuna que les permita organizar sus actividades: el régimen de trabajo y descanso de acuerdo con los objetivos de un estilo de vida saludable y la corrección de la salud. El objetivo es determinar los criterios y componentes de un recurso de información que será adecuado para los objetivos de mantener la salud de los adultos. Los enfoques principales para la investigación de los medios de comunicación de atención médica son: enfoques estructural-analíticos, orientados a la personalidad y culturales. Métodos de investigación: análisis y síntesis de la literatura científica y metodológica y el entorno de información sobre los problemas de mejora de la salud de la población y métodos de procesamiento de datos matemáticos. El artículo presenta los resultados de la determinación de los criterios para monitorear el ecosistema de información en un estilo de vida saludable, entre estos criterios se identificaron los siguientes: la validez científica de la información, la focalización, la importancia social y el diálogo. Además, se identificaron portales de datos que contribuyen a la solución de ciertas tareas de mejora de la salud de la población adulta. Los autores diseñaron e implementaron el portal informativo y educativo "3 jóvenes" que está dirigido a la implementación integrada de las necesidades básicas de mejora de la salud de la población adulta, teniendo en cuenta la interconexión de los componentes de salud: física, mental y social. Se presentan y discuten los resultados que prueban la efectividad del uso del portal de los autores para garantizar un estilo de vida saludable para los adultos. El artículo será útil para adultos que desean llevar un estilo de vida saludable con el apoyo de los recursos de información, maestros y formadores que trabajan con personas de edad madura. Este artículo también es útil para los maestros de la Facultad de Educación Física que planean promover un estilo de vida saludable entre las diferentes categorías de edad. El portal también será útil en el sistema de educación profesional adicional de instructores y entrenadores de fitness. La novedad de la investigación del autor consiste en la aplicación de enfoques metodológicos y criterios con base científica para monitorear el ecosistema de información relacionado con la organización de un estilo de vida saludable para adultos; en el desarrollo y prueba de un portal de información que resuelve un complejo de tareas para garantizar un estilo de vida saludable para los adulto
Structure of SAICAR synthase from Thermotoga maritima at 2.2 Å reveals an unusual covalent dimer
The crystal structure of phophoribosylaminoimidazole-succinocarboxamide or SAICAR synthase from T. maritima at 2.2 Å revealed an unusual covalent dimer
One-pot biocatalytic transformation of adipic acid to 6-aminocaproic acid and 1,6-hexamethylenediamine using carboxylic acid reductases and transaminases
The black market of personal data: financial, legal and social aspects
This article touches on the topic of the black market of personal data. The concept of the ”black market of personal data” and the description of its financial, legal and social aspects are considered. The relevance of this topic lies in the fact that offers on the black market have not only not decreased, on the contrary, their number has visibly increased. This article describes the basic rules for the security of personal data storage. Keywords: black market, personal data, user, messenger, darknet, information, database
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