Wear of Tailored Forming Steels

This study investigates the wear behavior of additively welded cladding layers on less wear-resistant base materials using plasma-transferred arc welding and laser hot-wire cladding. The cladding layers are made from atomized AISI 52100, AISI 5140, and a stainless steel with (0.52 wt% C, 0.9 wt% Si, 14 wt% Cr, 0.4 wt% Mo, 1.8 wt% Ni, 1.2 wt% V, bal. Fe) on unalloyed steel AISI 1022M as the base material. The specimens' microstructure and surface hardness are comparable with conventional specimens of monolithic AISI 52100 and AISI 4140, which is used as a reference. Tribometer tests are carried out in ball-on-disk configuration to investigate the wear resistance of the specimen. The multimaterial specimens show comparable wear behavior to their monolithic counterparts, and a good performance of the stainless specimen in pure sliding is proven. These findings suggest that additive manufacturing processes can be used to clad less wear-resistant base materials and achieve high wear resistance, making it possible to exploit the advantages of surface coatings under severe wear conditions

Closed cannulation of subclavian vein vs open cut-down of cephalic vein for totally implantable venous access port (TIVAP) implantation: protocol for a systematic review and proportional meta-analysis of perioperative and postoperative complications

Background: Totally implantable venous access port (TIVAP) implantation is one of the most often performed operations in general surgery (over 100,000/year in Germany). The two main approaches for TIVAP placement are insertion into the cephalic vein through an open cut-down technique (OCD) or closed cannulation technique of the subclavian vein (CC) with Seldinger technique. Both procedures are performed with high success rates and very low complication frequencies. Because of the low incidence of complications, no single interventional trial is able to report a valid comparison of peri- and postoperative complication frequencies between both techniques. Therefore, the aim of this systematic review is to summarize evidence for peri- and postoperative complication rates in patients undergoing OCD or CC. Methods/Design: A systematic literature search will be conducted in The Cochrane Library, MEDLINE, and Embase to identify randomized controlled trials (RCTs), observational clinical studies (OCS), or case series (CS) reporting peri- and/or postoperative complications of at least one implantation technique. A priori defined data will be extracted from included studies, and methodological quality will be assessed. Event rates with their 95% confidence intervals will be derived taking into account the follow-up time per study by patient-months where appropriate. Pooled estimates of event rates with corresponding 95% confidence intervals will be calculated on the base of the Freeman-Tukey double arcsine transformation within a random effect model framework. Discussion: The findings of this systematic review with proportional meta-analysis will help to identify the procedure with the best benefit/risk ratio for TIVAP implantation. This may have influence on daily practice, and data may be implemented in treatment guidelines. Considering the impact of TIVAP implantation on patients’ well being together with its socioeconomic relevance, patients will benefit from evidence-based treatment and health-care costs may also be reduced. Systematic review registration PROSPERO CRD42013005180

Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage

Introduction: The role of CD3−CD56+ natural killer (NK) cells in granulomatosis with polyangiitis (GPA) is poorly understood. Recently, it has been shown that peripheral blood NK cells can kill renal microvascular endothelial cells, suggesting a pathogenic role of NK cells in this disease. So far, subset distribution, phenotype, and function of peripheral blood NK cells in relation to GPA disease activity have not been elucidated. Moreover, it is not known whether NK cells infiltrate GPA tissue lesions. Methods: Paraffin sections of GPA granulomas and controls were stained with anti-CD56 and anti-CD3 antibodies. Peripheral blood lymphocyte subsets were analyzed by flow cytometry. NK cell degranulation was analyzed using cocultures of patient PBMCs with target cells and surface expression of CD107a. Clinical data were extracted from medical records. Statistical analysis was performed in an exploratory way. Results: CD56+ cells were not detectable in active granulomatous GPA lesions but were found frequently in granulomas from tuberculosis and sarcoidosis patients. In GPA, the proportion of NK cells among peripheral blood lymphocytes correlated negatively with the Birmingham Vasculitis Activity Score (BVAS) (n = 28). Accordingly, NK cell percentages correlated positively with the duration of remission (n = 28) and were significantly higher in inactive GPA (BVAS = 0, n = 17) than in active GPA, healthy controls (n = 29), and inactive control diseases (n = 12). The highest NK cell percentages were found in patients with long-term remission and tapered immunosuppressive therapy. NK cell percentages >18.5 % of peripheral blood lymphocytes (n = 12/28) determined GPA inactivity with a specificity of 100 %. The differentiation into CD56dim and CD56bright NK cell subsets was unchanged in GPA (n = 28), irrespective of disease activity. Similar surface expression of the activating NK cell-receptors (NKp30, NKp46, and NKG2D) was determined. Like in healthy controls, GPA NK cells degranulated in the presence of NK cell receptor ligand bearing epithelial and lymphatic target cells. Conclusions: NK cells were not detectable in GPA granulomas. Peripheral blood NK cell percentages positively correlate with the suppression of GPA activity and could serve as a biomarker for GPA activity. Peripheral blood NK cells in GPA patients are mature NK cells with preserved immune recognition

Influence of degree of deformation on welding pore reduction in high-carbon steels

Locally adapted properties within a machine component offer opportunities to increase the performance of a component by using high strenght materials where they are needed. The economic production of such hybrid components on the other hand represents a major challenge. The new tailored forming process chain, which is developed within the collaborative research center (CRC 1153) represents a possible solution to produce hybrid components. This is made possible by the use of pre-joined hybrid semi-finished products made from two different steel alloys, which are subsequently formed. The semi-finished products can be manufactured for example by means of deposition welding. Due to a thermal mechanical treatment, an overall higher component strength of the joining zone can be achieved. The deposition welding processes can be used to generate a cladding on a base material. During the welding, one of the most difficult tasks is to reduce the amount and size of pores in the joining zone. These pores can reduce the strength in the joining zone of the welded parts. However, additional pores can occur in the intermediate zone between the substrate and the cladding. In the presented study, the influence of the forming process on the closing of pores in the cladding and in the intermediate zone was investigated. Therefore, cylindrical specimen were extracted in longitudinal direction of the welding track by wire-cut eroding. These welding tracks are manufactured by plasma-transferred arc welding of AISI 52100 on a base plate made of AISI 1015. Further, specimens were prepared transversely, so that the base material, the intermediate layer, and the welded material are axially arranged in the specimen. The prepared specimen were checked for pores by means of scanning acoustic microscopy. Subsequently, an uniaxial compression test was carried out with various degrees of deformation and the two specimen designs were examined again for pores. A microstructure analysis was carried out after each step. The investigations show that there is a need for a minimum degree of deformation to reduce pores in the welded material. However, this required plastic strain cannot be achieved in the welded material of the hybrid specimen, which is a result of the homogeneous temperature distribution in the specimen. The homogeneous temperature distribution leads to different flow properties in the specimen, which means that the main plastic deformation is taking place in the base material. © 2021, The Author(s)

Investigations on Additively Manufactured Stainless Bearings

Additive manufacturing with multi-material design offers great possibilities for lightweight and function-integrated components. A process chain was developed in which hybrid steel–steel-components with high fatigue strength were produced. For this, a material combination of stainless powder material Rockit® (0.52 wt.% C, 0.9% Si, 14% Cr, 0.4% Mo, 1.8% Ni, 1.2% V, bal. Fe) cladded onto ASTM A572 mild steel by plasma arc powder deposition welding was investigated. Extensive material characterization has shown that defect-free claddings can be produced by carefully adjusting the welding process. With a tailored heat treatment strategy and machining of the semi-finished products, bearing washers for a thrust cylindrical roller bearing were produced. These washers showed a longer fatigue life than previously produced bearing washers with AISI 52100 bearing steel as cladding. It was also remarkable that the service life with the Rockit® cladding material was longer than that of conventional monolithic AISI 52100 washers. This was reached through a favourable microstructure with finely distributed vanadium and chromium carbides in a martensitic matrix as well as the presence of compressive residual stresses, which are largely retained even after testing. The potential for further enhancement of the cladding performance through Tailored Forming was investigated in compression and forging tests and was found to be limited due to low forming capacity of the material

Investigations on tailored forming of aisi 52100 as rolling bearing raceway

Hybrid cylindrical roller thrust bearing washers of type 81212 were manufactured by tailored forming. An AISI 1022M base material, featuring a sufficient strength for structural loads, was cladded with the bearing steel AISI 52100 by plasma transferred arc welding (PTA). Though AISI 52100 is generally regarded as non-weldable, it could be applied as a cladding material by adjusting PTA parameters. The cladded parts were investigated after each individual process step and subsequently tested under rolling contact load. Welding defects that could not be completely eliminated by the subsequent hot forming were characterized by means of scanning acoustic microscopy and micrographs. Below the surface, pores with a typical size of ten µm were found to a depth of about 0.45 mm. In the material transition zone and between individual weld seams, larger voids were observed. Grinding of the surface after heat treatment caused compressive residual stresses near the surface with a relatively small depth. Fatigue tests were carried out on an FE8 test rig. Eighty-two percent of the calculated rating life for conventional bearings was achieved. A high failure slope of the Weibull regression was determined. A relationship between the weld defects and the fatigue behavior is likely. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Graphene Oxide Framework Structures and Coatings: Impact on Cell Adhesion and Pre-Vascularization Processes for Bone Grafts

Graphene oxide (GO) is a promising material for bone tissue engineering, but the validation of its molecular biological effects, especially in the context of clinically applied materials, is still limited. In this study, we compare the effects of graphene oxide framework structures (F-GO) and reduced graphene oxide-based framework structures (F-rGO) as scaffold material with a special focus on vascularization associated processes and mechanisms in the bone. Highly porous networks of zinc oxide tetrapods serving as sacrificial templates were used to create F-GO and F-rGO with porosities >99% consisting of hollow interconnected microtubes. Framework materials were seeded with human mesenchymal stem cells (MSC), and the cell response was evaluated by confocal laser scanning microscopy (CLSM), deoxyribonucleic acid (DNA) quantification, real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and alkaline phosphatase activity (ALP) to define their impact on cellular adhesion, osteogenic differentiation, and secretion of vascular growth factors. F-GO based scaffolds improved adhesion and growth of MSC as indicated by CLSM and DNA quantification. Further, F-GO showed a better vascular endothelial growth factor (VEGF) binding capacity and improved cell growth as well as the formation of microvascular capillary-like structures in co-cultures with outgrowth endothelial cells (OEC). These results clearly favored non-reduced graphene oxide in the form of F-GO for bone regeneration applications. To study GO in the context of a clinically used implant material, we coated a commercially available xenograft (Bio-Oss® block) with GO and compared the growth of MSC in monoculture and in coculture with OEC to the native scaffold. We observed a significantly improved growth of MSC and formation of prevascular structures on coated Bio-Oss®, again associated with a higher VEGF binding capacity. We conclude that graphene oxide coating of this clinically used, but highly debiologized bone graft improves MSC cell adhesion and vascularization

Investigations on Additively Manufactured Stainless Bearings

Additive manufacturing with multi-material design offers great possibilities for lightweight and function-integrated components. A process chain was developed in which hybrid steel–steel-components with high fatigue strength were produced. For this, a material combination of stainless powder material Rockit® (0.52 wt.% C, 0.9% Si, 14% Cr, 0.4% Mo, 1.8% Ni, 1.2% V, bal. Fe) cladded onto ASTM A572 mild steel by plasma arc powder deposition welding was investigated. Extensive material characterization has shown that defect-free claddings can be produced by carefully adjusting the welding process. With a tailored heat treatment strategy and machining of the semi-finished products, bearing washers for a thrust cylindrical roller bearing were produced. These washers showed a longer fatigue life than previously produced bearing washers with AISI 52100 bearing steel as cladding. It was also remarkable that the service life with the Rockit® cladding material was longer than that of conventional monolithic AISI 52100 washers. This was reached through a favourable microstructure with finely distributed vanadium and chromium carbides in a martensitic matrix as well as the presence of compressive residual stresses, which are largely retained even after testing. The potential for further enhancement of the cladding performance through Tailored Forming was investigated in compression and forging tests and was found to be limited due to low forming capacity of the material