Taxonomic investigation of Ralfsia-like (Ralfsiales, Phaeophyceae) taxa in the North Atlantic Ocean based on molecular and morphological data, with descriptions of Pseudoralfsiaceae fam. nov., Pseudoralfsia azorica gen. et sp. nov. and Nuchella vesicularis gen. et sp. nov.

In this study we investigatedRalfsia-like crusts (i.e. excludingRalfsiasensu stricto,Stragulariaspp. and/or Scytosiphonaceae crustose phases) with an emphasis on the North Atlantic Ocean using molecular data (COI-5P, ITS andrbcL-3P) combined with morpho-anatomical comparisons of type material and contemporary specimens. Of the four species ofRalfsiapreviously reported in Europe, onlyR. fungiformisis presently recognized as belonging toRalfsiasensu stricto, Ralfsiaceae, with the remaining species,R. lucida, R. ovataandR. verrucosa, of uncertain taxonomic status. Our study revealed 11 independent genetic lineages ofRalfsia-like taxa, which were not assignable to any of the recognized families of the Ralfsiales. To accommodate this diversity, we propose Pseudoralfsiaceae Parente, Fletcher & G.W.Saunders fam. nov., including two new generaPseudoralfsiaParente, Fletcher & G.W.Saunders gen. nov. andNuchellaParente, Fletcher & G.W.Saunders gen. nov. The first genus includesPseudoralfsia verrucosa(Areschoug) Parente, Fletcher & G.W.Saunders comb. nov. as the generitype (the only species of the three remaining European species of uncertain taxonomic status assigned toRalfsiathat was reassessed here),P. azoricaParente, Fletcher & G.W.Saunders sp. nov. and seven undescribed genetic groups, which require additional vouchers for description. The second genus has as generitypeNuchella vesicularisParente, Fletcher & G.W.Saunders sp. nov., and also includesN. sp._1MP, which was represented by a single sterile specimen from Swanage, UK and consequently not characterized. Three characteristics differentiateNuchellafromPseudoralfsia- the former typically with numerous vesicles, non-synchronous development of the plurangia and hair pits arising from both middle and lower cells of the erect filaments. Species of Pseudoralfsiaceae can be distinguished fromRalfsiasensu stricto (Ralfsiaceae) mainly by DNA sequences and by consistently having frequent hair pits, and typically unsymmetrical thalli.This research was funded by FEDER funds through the Programa Operacional Factores de Competitividade - COMPETE and by national funds through FCT - Foundation for Science and Technology in the scope of the MACROBIOMOL project (ref. PTDC/MAR/114613/2009) and under the UID/BIA/50027/2013 and POCI-010145-FEDER-006821 programmes. Work completed in the Saunders Laboratory was supported by the Canadian Barcode of Life Network from Genome Canada in association with the Ontario Genomics Institute, the Natural Sciences and Engineering Research Council of Canada, as well as other sponsors listed at www.boldsystems.org, with infrastructure support from the Canada Foundation for Innovation and New Brunswick Innovation Foundation

How do people with diabetes describe their experiences in primary care? Evidence from 85,760 patients with self-reported diabetes from the English General Practice Patient Survey.

OBJECTIVE: Developing primary care is an important current health policy goal in the U.S. and England. Information on patients' experience can help to improve the care of people with diabetes. We describe the experiences of people with diabetes in primary care and examine how these experiences vary with increasing comorbidity. RESEARCH DESIGN AND METHODS: Using data from 906,578 responders to the 2012 General Practice Patient Survey (England), including 85,760 with self-reported diabetes, we used logistic regressions controlling for age, sex, ethnicity, and socioeconomic status to analyze patient experience using seven items covering three domains of primary care: access, continuity, and communication. RESULTS: People with diabetes were significantly more likely to report better experience on six out of seven primary care items than people without diabetes after adjusting for age, sex, ethnicity, and socioeconomic status (adjusted differences 0.88-3.20%; odds ratios [ORs] 1.07-1.18; P < 0.001). Those with diabetes and additional comorbid long-term conditions were more likely to report worse experiences, particularly for access to primary care appointments (patients with diabetes alone compared with patients without diabetes: OR 1.22 [95% CI 1.17-1.28] and patients with diabetes plus three or more conditions compared with patients without diabetes: OR 0.87 [95% CI 0.83-0.91]). CONCLUSIONS: People with diabetes in England report primary care experiences that are at least as good as those without diabetes for most domains of care. However, improvements in primary care are needed for diabetes patients with comorbid long-term conditions, including better access to appointments and improved communication.Diabetes UKThis is the author accepted manuscript. The final version is available from the American Diabetes Association via http://dx.doi.org/10.2337/dc14-109

The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery

&lt;p&gt;Background: The small ruminant parasite Haemonchus contortus is the most widely used parasitic nematode in drug discovery, vaccine development and anthelmintic resistance research. Its remarkable propensity to develop resistance threatens the viability of the sheep industry in many regions of the world and provides a cautionary example of the effect of mass drug administration to control parasitic nematodes. Its phylogenetic position makes it particularly well placed for comparison with the free-living nematode Caenorhabditis elegans and the most economically important parasites of livestock and humans.&lt;/p&gt; &lt;p&gt;Results: Here we report the detailed analysis of a draft genome assembly and extensive transcriptomic dataset for H. contortus. This represents the first genome to be published for a strongylid nematode and the most extensive transcriptomic dataset for any parasitic nematode reported to date. We show a general pattern of conservation of genome structure and gene content between H. contortus and C. elegans, but also a dramatic expansion of important parasite gene families. We identify genes involved in parasite-specific pathways such as blood feeding, neurological function, and drug metabolism. In particular, we describe complete gene repertoires for known drug target families, providing the most comprehensive understanding yet of the action of several important anthelmintics. Also, we identify a set of genes enriched in the parasitic stages of the lifecycle and the parasite gut that provide a rich source of vaccine and drug target candidates.&lt;/p&gt; &lt;p&gt;Conclusions: The H. contortus genome and transcriptome provides an essential platform for postgenomic research in this and other important strongylid parasites. &lt;/p&gt

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum