Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research

Forschendes Lernen im Fach Latein

Sauer J. Forschendes Lernen im Fach Latein. In: Schüssler R, Schöning A, Schwier V, eds. Im Praxissemester forschend lernen - Umsetzung in Schule, Universität und Studienseminar. Bad Heilbrunn: Verlag Julius Klinkhardt; 2016: 286-291

Clemens Brentano, Sämtliche Werke und Briefe. Historisch-Kritische Ausgabe. Band 21,1, Prosa VI,1: Satiren und Kleine Prosa

Testi satirici dell'autore romantico Clemens Brentano

Empirische Studienprojekte im Fach Latein

Sauer J. Empirische Studienprojekte im Fach Latein. In: Schüssler R, Schöning A, Schwier V, Schicht S, Gold J, Weyland U, eds. Forschendes Lernen im Praxissemester - Zugänge, Konzepte, Erfahrungen. Bad Heilbrunn: Klinkhardt; 2017: 286-291

The 8 year functional and symptomatic outcome of first episode psychosis (FEP)

The study purpose is to examine the 8-year functional and symptomatic outcome of a FEP cohort of 800 patients in addition to the course patterns of psychosis over time. The design is a naturalistic, prospective, longitudinal, 8-year follow-up study with multiple follow- up time points, on a representative multidiagnostic cohort of 800 patients with FEP from the Early Psychosis Prevention and Intervention Centre, which is a frontline public mental health service and its precursor service in Melbourne, Australia. Recruitment is ongoing. The preliminary results which follow concern the 470 subjects who have been interviewed to date. At 8-year outcome, analyses indicate that 74% remain unmarried, 54% are either working, studying or are homemakers. Mean scores on quality of life and social functioning measures indicate functioning in the good range. 75% are currently receiving psychiatric treatment, however 45% of these were being treated in the private health sector suggesting less severe illness and better functioning. Those taking an antipsychotic were on a CPZ equivalent mean low dose of 303mg. In terms of psychiatric hospital admission, 60% have not been admitted in the most recent two years and the mean number of admissions over the 8-year period was two. 47% have not been psychotic in the most recent 2 years and one quarter never had another psychotic episode after recovering from their first episode. Mean scores on psychopathology measures indicate minimal to low levels of current psychopathology. Comparison of these findings with the existing outcome literature of similar follow- up duration should be made with caution as the majority are of first episode schizophrenia. However our data concerning course type over the most recent two years is consistent with the findings of Mason et al. (1995) and Robinson et al. (2004). However our findings concerning the never psychotic course type over the entire follow- up period differs to that found by Thara et al. (1994). Our study reported a higher percentage (24%) than Thara et al. (1994) (17%) who had not been psychotic during the intervening years and over half of this group had completely remitted. Over half of our cohort was fully occupationally engaged compared with 19% reported by the Scottish Schizophrenia Research Group (1992). The findings from this study suggest patients with psychotic disorders can achieve symptomatic remission and good social and occupational functioning 8-years post FEP

Enzyme kinetics informatics : from instrument to browser

A limited number of publicly available resources provide access to enzyme kinetic parameters. These have been compiled through manual data mining of published papers, not from the original, raw experimental data from which the parameters were calculated. This is largely due to the lack of software or standards to support the capture, analysis, storage and dissemination of such experimental data. Introduced here is an integrative system to manage experimental enzyme kinetics data from instrument to browser. The approach is based on two interrelated databases: the existing SABIO-RK database, containing kinetic data and corresponding metadata, and the newly introduced experimental raw data repository, MeMo-RK. Both systems are publicly available by web browser and web service interfaces and are configurable to ensure privacy of unpublished data. Users of this system are provided with the ability to view both kinetic parameters and the experimental raw data from which they are calculated, providing increased confidence in the data. A data analysis and submission tool, the kineticswizard, has been developed to allow the experimentalist to perform data collection, analysis and submission to both data resources. The system is designed to be extensible, allowing integration with other manufacturer instruments covering a range of analytical techniques