104 research outputs found

    Sediment control and logs capturing in sand pocket with combination of sabo dam with large conduit and iron bars

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    In the Otoishi River, a tributary on right side of the Akatani River in the Chikugo River basin, a large amount of sediment and woody debris were deposited on riverbed due to the sediment disaster caused by the heavy rainfall in northern part of Kyushu Region on 5th to 6th July in 2017. As one of countermeasures to control the outflow of sediment and woody debris to the Akatani River, a sand pocket is planned with the sabo dam with large conduit parts and the log broom works. In this study, hydraulic model test and flume test were carried out to obtain knowledges for design of the sand pocket. Iron bars are installed vertically at the upstream front of the large conduit parts for capturing sediment and logs, and those intervals are desirable to be set in consideration of sediment diameter and sediment movement without the dam. Besides, it is necessary to consider capturing function and flow characteristics such as shockwave when the log broom works is planned in setting of layout and dimensions

    Laser-induced-fluorescence measurement of thermal conductivity in warm dense matter generated by pulsed-power discharge

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    Thermal conductivity in warm dense matter is one of the interests for thermonuclear fusion scenarios. Alternative inertial confinement fusion, which is a fast ignition with applied magnetic field [1], has been considered to improve the coupling efficiency. The target behavior of the fast ignition with applied magnetic field depends on the anisotropic thermal conductivity. The magnetic confinement fusion (MCF) [2] Up to now, the heat load on the divertor in previous MCF systems has been unreached parameter. Thus, to predict properties of the divertor under these heat loads, several experiments have been performed using several methods[3-6]. To predict the performance of the tungsten divertor in MCF, we should analyze not only metallurgical properties but also thermophysical properties of ablated tungsten..

    Lifestyle carbon footprints and changes in lifestyles to limit global warming to 1.5 °C, and ways forward for related research

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    This paper presents an approach for assessing lifestyle carbon footprints and lifestyle change options aimed at achieving the 1.5 °C climate goal and facilitating the transition to decarbonized lifestyles through stakeholder participatory research. Using data on Finland and Japan it shows potential impacts of reducing carbon footprints through changes in lifestyles for around 30 options covering food, housing, and mobility domains, in comparison with the 2030 and 2050 per-capita targets (2.5-3.2 tCO2e by 2030; 0.7-1.4 tCO2e by 2050). It discusses research opportunities for expanding the footprint-based quantitative analysis to incorporate subnational analysis, living lab, and scenario development aiming at advancing sustainability science on the transition to decarbonized lifestyles

    Laser-induced-fluorescence measurement of thermal conductivity in warm dense matter generated by pulsed-power discharge

    Get PDF
    Thermal conductivity in warm dense matter is one of the interests for thermonuclear fusion scenarios. Alternative inertial confinement fusion, which is a fast ignition with applied magnetic field [1], has been considered to improve the coupling efficiency. The target behavior of the fast ignition with applied magnetic field depends on the anisotropic thermal conductivity. The magnetic confinement fusion (MCF) [2] Up to now, the heat load on the divertor in previous MCF systems has been unreached parameter. Thus, to predict properties of the divertor under these heat loads, several experiments have been performed using several methods[3-6]. To predict the performance of the tungsten divertor in MCF, we should analyze not only metallurgical properties but also thermophysical properties of ablated tungsten..

    Molecular phylogeny of the rotifers with two Indonesian Brachionus lineages

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    The rotifer Brachionus plicatilis is an ecologically and commercially important species, and has been studied in various fields such as population dynamics, ecotoxicology and aging. However, recent studies have revealed that the B. plicatilis lineages involve an unknown number of cryptic species, and the group has been regarded as the Brachionus complex. One cause of this complicated taxonomy is the lack of surveys in the tropical zone, which is characterized by enormous species-richness. Accordingly, in this study we collected two Brachionus rotifers from the Sumatra and Sulawesi Islands, Indonesia, and determined their partial nucleotide sequences of mitochondrial DNA cytochrome c oxidase subunit I gene. Subsequently, we constructed molecular phylogenetic trees with fourteen species/lineages from four genera including the two Indonesian rotifers. The two Indonesian Brachionus rotifers were respectively found to be phylogenetically close to B. ibericus and B. rotundiformis. On the other hand, Japanese B. plicatilis was suggested to be phylogenetically closer to B. Manjavacas, which is proposed to be a new species, than to Spanish B. plicatilis. These results imply that the current taxonomy of the Brachionus is problematic, and a major revision is necessary to establish a reliable taxonomy of this group

    Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD.

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    Sequential mannose trimming of N-glycan, from M9 to M8B and then to oligosaccharides exposing the α1,6-linked mannosyl residue (M7A, M6, and M5), facilitates endoplasmic reticulum-associated degradation of misfolded glycoproteins (gpERAD). We previously showed that EDEM2 stably disulfide-bonded to the thioredoxin domain-containing protein TXNDC11 is responsible for the first step (George et al., 2020). Here, we show that EDEM3 and EDEM1 are responsible for the second step. Incubation of pyridylamine-labeled M8B with purified EDEM3 alone produced M7 (M7A and M7C), M6, and M5. EDEM1 showed a similar tendency, although much lower amounts of M6 and M5 were produced. Thus, EDEM3 is a major α1,2-mannosidase for the second step from M8B. Both EDEM3 and EDEM1 trimmed M8B from a glycoprotein efficiently. Our confirmation of the Golgi localization of MAN1B indicates that no other α1,2-mannosidase is required for gpERAD. Accordingly, we have established the entire route of oligosaccharide processing and the enzymes responsible

    Highly Sensitive Determination of Hydrogen Peroxide and Glucose by Fluorescence Correlation Spectroscopy

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    BACKGROUND: Because H(2)O(2) is generated by various oxidase-catalyzed reactions, a highly sensitive determination method of H(2)O(2) is applicable to measurements of low levels of various oxidases and their substrates such as glucose, lactate, glutamate, urate, xanthine, choline, cholesterol and NADPH. We propose herein a new, highly sensitive method for the measurement of H(2)O(2) and glucose using fluorescence correlation spectroscopy (FCS). METHODOLOGY/PRINCIPAL FINDINGS: FCS has the advantage of allowing us to determine the number of fluorescent molecules. FCS measures the fluctuations in fluorescence intensity caused by fluorescent probe movement in a small light cavity with a defined volume generated by confocal illumination. We thus developed a highly sensitive determination system of H(2)O(2) by FCS, where horseradish peroxidase (HRP) catalyzes the formation of a covalent bond between fluorescent molecules and proteins in the presence of H(2)O(2). Our developed system gave a linear calibration curve for H(2)O(2) in the range of 28 to 300 nM with the detection limit of 8 nM. In addition, by coupling with glucose oxidase (GOD)-catalyzed reaction, the method allows to measure glucose in the range of 80 nM to 1.5 µM with detection limit of 24 nM. The method was applicable to the assay of glucose in blood plasma. The mean concentration of glucose in normal human blood plasma was determined to be 4.9 mM. CONCLUSIONS/SIGNIFICANCE: In comparison with commercial available methods, the detection limit and the minimum value of determination for glucose are at least 2 orders of magnitude more sensitive in our system. Such a highly sensitive method leads the fact that only a very small amount of plasma (20 nL) is needed for the determination of glucose concentration in blood plasma
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