Molecular phylogeny and evolution of <i>Parabasalia</i> with improved taxon sampling and new protein markers of actin and elongation factor-1α

Background: Inferring the evolutionary history of phylogenetically isolated, deep-branching groups of taxa—in particular determining the root—is often extraordinarily difficult because their close relatives are unavailable as suitable outgroups. One of these taxonomic groups is the phylum Parabasalia, which comprises morphologically diverse species of flagellated protists of ecological, medical, and evolutionary significance. Indeed, previous molecular phylogenetic analyses of members of this phylum have yielded conflicting and possibly erroneous inferences. Furthermore, many species of Parabasalia are symbionts in the gut of termites and cockroaches or parasites and therefore formidably difficult to cultivate, rendering available data insufficient. Increasing the numbers of examined taxa and informative characters (e.g., genes) is likely to produce more reliable inferences. Principal Findings: Actin and elongation factor-1a genes were identified newly from 22 species of termite-gut symbionts through careful manipulations and seven cultured species, which covered major lineages of Parabasalia. Their protein sequences were concatenated and analyzed with sequences of previously and newly identified glyceraldehyde-3-phosphate dehydrogenase and the small-subunit rRNA gene. This concatenated dataset provided more robust phylogenetic relationships among major groups of Parabasalia and a more plausible new root position than those previously reported. Conclusions/Significance: We conclude that increasing the number of sampled taxa as well as the addition of new sequences greatly improves the accuracy and robustness of the phylogenetic inference. A morphologically simple cell is likely the ancient form in Parabasalia as opposed to a cell with elaborate flagellar and cytoskeletal structures, which was defined as most basal in previous inferences. Nevertheless, the evolution of Parabasalia is complex owing to several independent multiplication and simplification events in these structures. Therefore, systematics based solely on morphology does not reflect the evolutionary history of parabasalids

<Preliminary>Components and Anti-fungal Efficiency of Wood-vinegar-liquor Prepared under Different Carbonization Conditions

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました

Dense Plasma Torus in the GPS Galaxy NGC 1052

We report results from nearly simultaneous pentachromatic VLBI observations towa rds a nearby GPS galaxy NGC 1052. The observations at 1.6 and 4.8 GHz with VSOP, and at 2.3, 8.4, and 15.4 GHz wit h VLBA, provide linear resolutions of $\sim 0.1$ pc. Convex spectra of a double-sided jet imply that synchrotron emission is obscured through foreground cold dense plasma, in terms of free--free absorption (FFA). We found a central condensation of the plasma which covers about 0.1 pc and 1 pc of the approaching and receding jets, respectively. A simple model with a geometrically thick plasma torus perpendicular to the jets is established to explain the asymmetric distribution of FFA opacities.Comment: 9 pages, 4 figures, other comment

Free-Free Absorption and the Unified Scheme

We report Very-Long-Baseline Array (VLBA) observations at 2.3, 8.4, and 15.4 GHz towards nine GHz-Peaked Spectrum (GPS) sources. One Seyfert 1 galaxy, one Seyfert 2 galaxy, three radio galaxies, and four quasars were included in our survey. We obtained spatial distributions of the Free-Free Absorption (FFA) opacity with milliarcsec resolutions for all sources. It is found that type-1 (Seyfert 1 and quasars) and type-2 (Seyfert 2 and radio galaxies) sources showed different distributions of the FFA opacities. The type-1 sources tend to show more asymmetric opacity distributions towards a double lobe, while those of the type-2 sources are rather symmetric. Our results imply that the different viewing angle of the jet causes the difference of FFA opacity along the external absorber. This idea supports the unified scheme between quasars and radio galaxies, proposed by Barthel (1989).Comment: 17 pages, including 8 figures. Proceedings of the 3rd Compact Steep Spectrum and GHz Peaked Spectrum Sources, 2002, Greece. Submitted to the Publications of Astronomical Society of Australi

Dislocated Narratives : Contemporary Polish Literature in the Federal Republic of Germany

学位の種別:課程博士University of Tokyo(東京大学

Positional Coincidence of H2O Maser and a Plasma Obscuring Torus in Radio Galaxy NGC 1052

We present multi-frequency simultaneous VLBA observations at 15, 22 and 43 GHz towards the nucleus of the nearby radio galaxy NGC 1052. These three continuum images reveal a double-sided jet structure, whose relative intensity ratios imply that the jet axis is oriented close to the sky plane. The steeply rising spectra at 15-43 GHz at the inner edges of the jets strongly suggest that synchrotron emission is absorbed by foreground thermal plasma. We detected H2O maser emission in the velocity range of 1550-1850 km/s, which is redshifted by 50-350 km/s with respect to the systemic velocity of NGC 1052. The redshifted maser gas appears projected against both sides of the jet, in the same manner as the HI seen in absorption. The H2O maser gas are located where the free-free absorption opacity is large. This probably imply that the masers in NGC 1052 are associated with a circumnuclear torus or disk as in the nucleus of NGC 4258. Such circumnuclear structure can be the sence of accreting onto the central engine.Comment: 18 pages, 7 figures, accepted for publication in Ap

Induction of inverted morphology in brain organoids by vertical-mixing bioreactors

上下動撹拌培養装置を用いた流体制御により誘導した反転型脳オルガノイド 脳オルガノイド誘導の流体シミュレーションと流体力学的理解. 京都大学プレスリリース. 2021-10-27.How you mix cells changes the brain. 京都大学プレスリリース. 2021-10-28.Organoid technology provides an opportunity to generate brain-like structures by recapitulating developmental steps in the manner of self-organization. Here we examined the vertical-mixing effect on brain organoid structures using bioreactors and established inverted brain organoids. The organoids generated by vertical mixing showed neurons that migrated from the outer periphery to the inner core of organoids, in contrast to orbital mixing. Computational analysis of flow dynamics clarified that, by comparison with orbital mixing, vertical mixing maintained the high turbulent energy around organoids, and continuously kept inter-organoid distances by dispersing and adding uniform rheological force on organoids. To uncover the mechanisms of the inverted structure, we investigated the direction of primary cilia, a cellular mechanosensor. Primary cilia of neural progenitors by vertical mixing were aligned in a multidirectional manner, and those by orbital mixing in a bidirectional manner. Single-cell RNA sequencing revealed that neurons of inverted brain organoids presented a GABAergic character of the ventral forebrain. These results suggest that controlling fluid dynamics by biomechanical engineering can direct stem cell differentiation of brain organoids, and that inverted brain organoids will be applicable for studying human brain development and disorders in the future

Comparison of Urinary Levels of 8-Hydroxy-2’-deoxyguanosine between Young Females with and without Depressive Symptoms during Different Menstrual Phases

This study aimed to clarify the association between depressive symptoms and a marker of oxidative stress-induced DNA damage in young females. Since the menstrual cycle may confound or modify this association, depressive symptoms and urinary levels of 8-hydroxy-2ʼ deoxyguanosine (8-OHdG) were evaluated during each menstrual phase. A total of 57 female fourth-year students (aged 21.6±0.8) from a Japanese health science university were studied. The menstrual cycle was divided into 3 phases:menstrual (days 1 to 3 after the onset of menses);proliferative (days 13 to 15);and secretory (days 24 to 26). Depressive symptoms were assessed by the self-rating depression scale (SDS). Positive depressive symptoms were defined as a score of 53 or more during 2 different menstrual phases. The association between the presence of depressive symptoms and 8-OHdG levels adjusting for the menstrual cycle was examined by two-way analysis of variance with the menstrual cycle (menstrual, proliferative, and secretory phases) as the within-individual factor. The menstrual cycle did not show a significant correlation with urinary 8-OHdG levels. On the other hand, the menstrual cycle-adjusted 8-OHdG level was significantly higher in those with depressive symptoms (7.01ng/mL) than in those without them (3.98ng/mL). The ROC curve analysis showed that urinary 8-OHdG levels had reasonably high discriminative performance throughout all the menstrual cycles (0.73-0.81;all p＜0.05). These results indicated the presence of oxidative stress in subjects with depressive symptoms independent of the menstrual cycle

Pathophysiology of unilateral asterixis due to thalamic lesion.

[Objective]:Unilateral asterixis has been reported in patients with thalamic lesion. This study aims at elucidating the pathophysiology of the thalamic asterixis. [Methods]:Two cases with unilateral asterixis caused by an infarction in the lateral thalamus were studied by analysing the asterixis-related cortical activities, transcranial magnetic stimulation (TMS) for motor cortex excitability and probabilistic diffusion tractography for the thalamo-cortical connectivity. [Results]:Averaging of electroencephalogram (EEG) time-locked to the asterixis revealed rhythmic oscillations of a beta band at the central area contralateral to the affected hand. TMS revealed a decrease in the motor evoked potential (MEP) amplitude and a prolongation of the silent period (SP). The anatomical mapping of connections between the thalamus and cortical areas using a diffusion-weighted image (DWI) showed that the lateral thalamus involved by the infarction was connected to the premotor cortex, the primary motor cortex (M1) and the primary somatosensory cortex (S1) of the corresponding hemisphere. [Conclusions]:The thalamic asterixis is mediated by the sensorimotor cortex, which is subjected to excessive inhibition as a result of the thalamic lesion involving the ventral lateral nucleus. [Significance]:This is the first demonstration of participation of the sensorimotor cortex in the generation of asterixis due to the lateral thalamic lesion