Detecting air pollutants trends using Mann-Kendall tests and Sen’s slope estimates

Recently, trend detection in ambient air pollutants has received a lot of interest, particularly in relation to climatic changes. Air pollutants data that were acquired from monitoring stations from 2015 to 2021 were used in the current investigation. The direction and size of the monotonic trend were determined using the Mann-Kendall test and Sen's slope estimator. The findings showed that there was significant fluctuation in different parameters over time. According to the study, SO2 and NO2 indicate a slightly increasing tendency with approximate annual concentrations of 6mg/m3 and 40mg/m3, respectively, whereas PM2.5 shows a decreasing trend with an approximate annual concentration of 130mg/m3. For all of Odisha's districts, PM10 exhibits no trend, with annual concentrations of about 90mg/m3. The study found that while NO2, PM2.5, and PM10 concentrations were significantly over the standard allowed limits while SO2 concentrations were significantly below them. Specific actions are needed to reduce these pollutants' emissions in Odisha

Plant-derived tetranortriterpenoid, methyl angolensate activates apoptosis and prevents ehrlich ascites carcinoma induced tumorigenesis in mice

Background: Cancer is a leading health problem throughout the world. For decades, natural plant products have been playing promising roles as anticancer agents. Objective: The present study aims to investigate the chemotherapeutic potential of Methyl Angolensate (MA), purified from Soymida febrifuga in mice bearing carcinoma and examines the molecular basis for its anticancer actions. Study Design: The inhibitory effects of MA treatment on the survival of mice bearing Carcinoma and adverse side effects of MA treatment in mice were analyzed. Methods: Tumor volume, life span, histopathology, Immunohistochemical (IHC) analysis, estimation of liver enzyme, alkaline phosphatase and metabolites, creatinine and urea. Results: Oral administration of MA in mice with Ehrlich Ascites Carcinoma showed significant inhibition of tumor growth compared to untreated mice. We observed a significant increase in the life span (∼4-fold) of tumor bearing animals following treatment with MA. MA affected tumor cell proliferation by activating intrinsic pathway of apoptosis without imparting any side effect on normal cells. MA treatment in mice showed no major side effects. Conclusion: MA treatment showed significant inhibition of tumor growth by inducing apoptosis as well increased life span of mice, with no adverse side effects to normal cells. Altogether, the present in vivo study provides new insights of MA serving as a cancer chemotherapeutic agent

Интеллектуальное кресло-робот со вспомогательными средствами связи с использованием откликов TEP и характеристик диапазона спектра более высокого порядка

In recent years, electroencephalography-based navigation and communication systems for differentially enabled communities have been progressively receiving more attention. To provide a navigation system with a communication aid, a customized protocol using thought evoked potentials has been proposed in this research work to aid the differentially enabled communities. This study presents the higher order spectra based features to categorize seven basic tasks that include Forward, Left, Right, Yes, NO, Help and Relax; that can be used for navigating a robot chair and also for communications using an oddball paradigm. The proposed system records the eight-channel wireless electroencephalography signal from ten subjects while the subject was perceiving seven different tasks. The recorded brain wave signals are pre-processed to remove the interference waveforms and segmented into six frequency band signals, i. e. Delta, Theta, Alpha, Beta, Gamma 1-1 and Gamma 2. The frequency band signals are segmented into frame samples of equal length and are used to extract the features using bispectrum estimation. Further, statistical features such as the average value of bispectral magnitude and entropy using the bispectrum field are extracted and formed as a feature set. The extracted feature sets are tenfold cross validated using multilayer neural network classifier. From the results, it is observed that the entropy of bispectral magnitude feature based classifier model has the maximum classification accuracy of 84.71 % and the value of the bispectral magnitude feature based classifier model has the minimum classification accuracy of 68.52 %.В последние годы все больше внимания уделяется навигационным и коммуникационным системам на основе электроэнцефалограммы головного мозга для сообществ с разными возможностями. Для предоставления навигационной системе вспомогательных средств связи в работе предложен настраиваемый протокол, использующий вызванные мыслительные потенциалы, чтобы помочь сообществам с разными возможностями. Представлены функции, основанные на спектрах более высокого порядка, для классификации семи основных задач, таких как Вперед, Влево, Вправо, Да, НЕТ, Помощь и Расслабление, которые можно использовать для управления креслом-роботом, а также для связи с использованием необычной парадигмы. Предлагаемая система записывает восьмиканальный беспроводной сигнал электроэнцефалографии от десяти субъектов, в то время как субъект воспринимал семь различных задач. Записанные сигналы мозговых волн предварительно обрабатываются для удаления интерференционных волн и сегментируются на сигналы шести частотных диапазонов: дельта, тета, альфа, бета, гамма 1-1 и гамма 2. Сигналы полосы частот сегментируются на выборки кадров равной длины и используются для извлечения признаков с использованием оценки биспектра. Кроме того, статистические характеристики, такие как среднее значение биспектральной величины и энтропия с использованием области биспектра, извлекаются и формируются как набор характеристик. Извлеченные наборы функций проходят десятикратную перекрестную проверку с использованием классификатора многослойной нейронной сети. Результаты показали, что энтропия модели классификатора на основе характеристик биспектральной величины имеет максимальную точность классификации 84,71 %, а среднее значение модели классификатора на основе характеристик биспектральной величины – минимальную точность классификации 68,52 %

Formation of a G-quadruplex at the BCL2 major breakpoint region of the t(14;18) translocation in follicular lymphoma

The t(14;18) translocation in follicular lymphoma is one of the most common chromosomal translocations. Most breaks on chromosome 18 are located at the 3′-UTR of the BCL2 gene and are mainly clustered in the major breakpoint region (MBR). Recently, we found that the BCL2 MBR has a non-B DNA character in genomic DNA. Here, we show that single-stranded DNA modeled from the template strand of the BCL2 MBR, forms secondary structures that migrate faster on native PAGE in the presence of potassium, due to the formation of intramolecular G-quadruplexes. Circular dichroism shows evidence for a parallel orientation for G-quadruplex structures in the template strand of the BCL2 MBR. Mutagenesis and the DMS modification assay confirm the presence of three guanine tetrads in the structure. 1H nuclear magnetic resonance studies further confirm the formation of an intramolecular G-quadruplex and a representative model has been built based on all of the experimental evidence. We also provide data consistent with the possible formation of a G-quadruplex structure at the BCL2 MBR within mammalian cells. In summary, these important features could contribute to the single-stranded character at the BCL2 MBR, thereby contributing to chromosomal fragility

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century