42 research outputs found
DeCiFering the elusive cancer cell fraction in tumor heterogeneity and evolution
The cancer cell fraction (CCF), or proportion of cancerous cells in a tumor containing a single-nucleotide variant (SNV), is a fundamental statistic used to quantify tumor heterogeneity and evolution. Existing CCF estimation methods from bulk DNA sequencing data assume that every cell with an SNV contains the same number of copies of the SNV. This assumption is unrealistic in tumors with copy-number aberrations that alter SNV multiplicities. Furthermore, the CCF does not account for SNV losses due to copy-number aberrations, confounding downstream phylogenetic analyses. We introduce DeCiFer, an algorithm that overcomes these limitations by clustering SNVs using a novel statistic, the descendant cell fraction (DCF). The DCF quantifies both the prevalence of an SNV at the present time and its past evolutionary history using an evolutionary model that allows mutation losses. We show that DeCiFer yields more parsimonious reconstructions of tumor evolution than previously reported for 49 prostate cancer samples
Skin Development during the Film Formation of Waterborne Acrylic Pressure-Sensitive Adhesives Containing Tackifying Resin
Morphology and elasticity of waterborne acrylic pressure-sensitive adhesives investigated with atomic force microscopy
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Single-cell tumor phylogeny inference with copy-number constrained mutation losses
© Springer Nature Switzerland AG 2020. Motivation: Single-cell DNA sequencing enables the measurement of somatic mutations in individual tumor cells, and provides data to reconstruct the evolutionary history of the tumor