2 research outputs found
Evaluation antimicrobial and antiadhesive properties of the biosurfactant lunasan produced by candida sphaerica UCP 0995
Different groups of biosurfactants exhibit
diverse properties and display a variety of physiological
functions in producer microorganisms; these include
enhancing the solubility of hydrophobic/water-insoluble
compound, heave metal binding, bacterial pathogenesis, cell
adhesion and aggregation, quorum sensing and biofilm formation.
Candida sphaerica was grown in a low cost medium,
consisting of distilled water supplemented with 9%
refinery residue of soybean oil and 9% corn steep liquor, for
144 h at 28 C and 150 rpm. The cell-free supernatant
obtained at the end of the experiments was submitted to
extraction, and afterward the biosurfactant was isolated
using methanol with a yield of 9 g l-1. The critical micelle
concentration of the biosurfactant was found to be
0.25 mg ml-1 with a surface tension of 25 mN m-1. Several
concentrations of the biosurfactant (0.625–10 mg ml-1)
were used to evaluate its antimicrobial and antiadhesive
activities against a variety of microorganisms. The biosurfactant
showed antimicrobial activity against Streptococcus
oralis (68%), Candida albicans (57%), and Staphylococcus
epidermidis(57.6%) for the highest concentration tested.
Furthermore, the biosurfactant at a concentration of
10 mg ml-1 inhibited the adhesion between 80 and 92% of
Pseudomonas aeruginosa, Streptococcus agalactiae,
Streptococcus sanguis12. Inhibition of adhesion with percentages
near 100% occurred for the higher concentrations
of biosurfactant used. Results gathered in this study point to a
potential use of the biosurfactant in biomedical applicationsThe authors acknowledge the funding agencies FACEPE, CNPq, FINEP, PRONEX, and UNICAMP for using their facilities and CAPES for sandwich doctorate scholarship
Proposed definition of competencies for surgical neuro-oncology training
Objective: The aim of this work is to define competencies and entrustable professional activities (EPAs) to be imparted within the framework of surgical neuro-oncological residency and fellowship training as well as the education of medical students. Improved and specific training in surgical neuro-oncology promotes neuro-oncological expertise, quality of surgical neuro-oncological treatment and may also contribute to further development of neuro-oncological techniques and treatment protocols. Specific curricula for a surgical neuro-oncologic education have not yet been established. Methods: We used a consensus-building approach to propose skills, competencies and EPAs to be imparted within the framework of surgical neuro-oncological training. We developed competencies and EPAs suitable for training in surgical neuro-oncology. Result: In total, 70 competencies and 8 EPAs for training in surgical neuro-oncology were proposed. EPAs were defined for the management of the deteriorating patient, the management of patients with the diagnosis of a brain tumour, tumour-based resections, function-based surgical resections of brain tumours, the postoperative management of patients, the collaboration as a member of an interdisciplinary and/or -professional team and finally for the care of palliative and dying patients and their families. Conclusions and Relevance: The present work should subsequently initiate a discussion about the proposed competencies and EPAs and, together with the following discussion, contribute to the creation of new training concepts in surgical neuro-oncology. © 2021, The Author(s)