AN ETHNOBOTANICAL SURVEY OF ANTI-MALARIAL PLANTS IN SOME HIGHLY MALARIA AFFECTED DISTRICTS OF ASSAM

Objective: An ethnobotanical survey of potent anti-malarial plants used by different tribes and communities of highly malaria affected seven districts of Assam, India.Methods: Personal discussions and group interviews were held with the traditional practitioners. Samples were collected and authentically identified. Detailed ethno knowledge on the plant parts used and mode of administration of the plant extracts was documented. Literature survey was carried out on identified plant species and analyzed for their medicinal importance.Results: A total of 22 plant species belonging to 20 botanical families were collected and identified from different locations of seven districts of Assam. Different plant parts such as leaves, root, flower, bark etc. were found to process through different basic methods (infusion, decoction, direct mechanical crushing and maceration) for extracting the anti-malarial crude phytochemicals by the traditional healers. Extensive literature on the broad spectrum medicinal values other than anti-malarial activity of the plant species suggested a strong scientific background of selecting those particular plants species by the traditional practitioners.Conclusion: Indigenous plant species with strong claim of the natural source of anti-malarial moieties were identified in the present ethno-botanical survey. All the 22 plant species investigated could be a potential source of new antimalarial therapies.Â

Evaluating the effect of measles and rubella mass vaccination campaigns on seroprevalence in India: a before-and-after cross-sectional household serosurvey in four districts, 2018-2020

BACKGROUND: India did phased measles-rubella supplementary immunisation activities (MR-SIAs; ie, mass-immunisation campaigns) targeting children aged 9 months to less than 15 years. We estimated measles-rubella seroprevalence before and after the MR-SIAs to quantify the effect on population immunity and identify remaining immunity gaps. METHODS: Between March 9, 2018 and March 19, 2020 we did community-based, cross-sectional serosurveys in four districts in India before and after MR-SIAs. 30 villages or wards were selected within each district, and one census enumeration block from each was selected as the survey cluster. Households were enumerated and 13 children in the younger age group (9 months to <5 years) and 13 children in the older ager group (5 to <15 years) were randomly selected by use of computer-generated random numbers. Serum samples were tested for IgG antibodies to measles and rubella viruses by enzyme immunoassay. FINDINGS: Specimens were collected from 2570 children before the MR-SIA and from 2619 children afterwards. The weighted MR-SIA coverage ranged from 73·7% to 90·5% in younger children and from 73·6% to 93·6% in older children. Before the MR-SIA, district-level measles seroprevalence was between 80·7% and 88·5% among younger children in all districts, and between 63·4% and 84·5% among older children. After the MR-SIA, measles seroprevalence among younger children increased to more than 90% (range 91·5 to 96·0) in all districts except Kanpur Nagar, in which it remained unchanged 80·4%. Among older children, measles seroprevalence increased to more than 90·0% (range 93·7% to 96·5%) in all districts except Hoshiarpur (88·7%). A significant increase in rubella seroprevalence was observed in all districts in both age groups, with the largest effect in Dibrugarh, where rubella seroprevalence increased from 10·6% to 96·5% among younger children. INTERPRETATION: Measles-rubella seroprevalence increased substantially after the MR-SIAs but the serosurvey also identified remaining gaps in population immunity. FUNDING: The Bill & Melinda Gates Foundation and Indian Council of Medical Research