33 research outputs found
Analysis of Substrate-Binding Elements in OxlT, the Oxalate:Formate Antiporter of Oxalobacter formigenes
34 – E.Coli Heat Stable Enterotoxin (ST) Inhibition of Nhe3 Occurs by Different Signaling Pathways Downstream of Cyclic Gmp in Murine Jejunum and Caco-2 Cells: A Warning About Assumed Equivalence
749 LPA/LPA5 Receptor Stimulation of NHE3 Involves Two Separate Events: Stimulation of NHE3 Exocytosis via EGFR and ERK and Dynamic Dissociation of NHE3 From the Microvillar (MV) Cytoskeleton/NHERF2 via PLC and a Novel PKC
Su1844 NHERF2 Contains a Phosphorylatable Ezrin Binding Regulatory Domain, a Newly Identified Motif
The NHERF2 Dependent Dynamic Ca2+/LPA Regulation of NHE3 Mobility and Interaction At the Epithelial Brush Border
189 Basal and Stimulated BB NHE3 Activity Requires Intracellular SNX27, a PDZDomain Containing Binding Partner of NHE3 That Regulates Endosome to Plasma Membrane Trafficking
Projection structure and molecular architecture of OxlT, a bacterial membrane transporter
The major facilitator superfamily (MFS) represents the largest collection of evolutionarily related members within the class of membrane ‘carrier’ proteins. OxlT, a representative example of the MFS, is an oxalate-transporting membrane protein in Oxalobacter formigenes. From an electron crystallographic analysis of two-dimensional crystals of OxlT, we have determined the projection structure of this membrane transporter. The projection map at 6 Å resolution indicates the presence of 12 transmembrane helices in each monomer of OxlT, with one set of six helices related to the other set by an approximate internal two-fold axis. The projection map reveals the existence of a central cavity, which we propose to be part of the pathway of oxalate transport. By combining information from the projection map with related biochemical data, we present probable models for the architectural arrangement of transmembrane helices in this protein superfamily