Saccharomyces boulardii CNCM I-745 supplementation reduces gastrointestinal dysfunction in an animal model of IBS

We evaluated the effect of Saccharomyces boulardii CNCM I-745 on intestinal neuromuscular anomalies in an IBS-type mouse model of gastrointestinal motor dysfunctions elicited by Herpes Simplex Virus type 1 (HSV-1) exposure.Mice were inoculated intranasally with HSV-1 (102 PFU) or vehicle at time 0 and 4 weeks later by the intragastric (IG) route (108 PFU). Six weeks after IG inoculum, mice were randomly allocated to receive oral gavage with either S. boulardii (107 CFU/day) or vehicle. After 4 weeks the following were determined: a) intestinal motility using fluorescein-isothiocyanate dextran distribution in the gut, fecal pellet expulsion, stool water content, and distal colonic transit of glass beads; b) integrity of the enteric nervous system (ENS) by immunohistochemistry on ileal whole-mount preparations and western blot of protein lysates from ileal longitudinal muscle and myenteric plexus; c) isometric muscle tension with electric field and pharmacological (carbachol) stimulation of ileal segments; and d) intestinal inflammation by levels of tumor necrosis factor α, interleukin(IL)-1β, IL-10 and IL-4.S. boulardii CNCM I-745 improved HSV-1 induced intestinal dysmotility and alteration of intestinal transit observed ten weeks after IG inoculum of the virus. Also, the probiotic yeast ameliorated the structural alterations of the ENS induced by HSV-1 (i.e., reduced peripherin immunoreactivity and expression, increased glial S100β protein immunoreactivity and neuronal nitric oxide synthase level, reduced substance P-positive fibers). Moreover, S. boulardii CNCM I-745 diminished the production of HSV-1 associated pro-inflammatory cytokines in the myenteric plexus and increased levels of anti-inflammatory interleukins.S. boulardii CNCM I-745 ameliorated gastrointestinal neuromuscular anomalies in a mouse model of gut dysfunctions typically observed with irritable bowel syndrome

High-quality digital 3D reconstruction of the terminal pathway of a heart stab wound

High-quality digital 3D reconstructions of microscopic findings have been involved in anatomical and histopathological research, but their potentialities in forensic pathology may also be of particular interest. We here present the use of such methods to furtherly analyse a heart stab wound in a suicidal case. External examination revealed multiple incised wounds on the wrists, three stab wounds at the neck and a single stab wound on the chest. At autopsy, injuries to the neck and wrists were superficial whereas the thoracic stab wound penetrated the chest wall and pericardium. Heart examination showed an 8-mm-long stab wound on the anterior surface of the left ventricle. Heart sectioning and inspection of the correspondent internal aspect of the left ventricle did not show a macroscopic injury, but the irregularity of the trabeculae carneae did not permit to exclude a microscopic pathway. Thus, the heart wall including the stab wound was paraffin-embedded and subjected to complete sectioning for microscopic analysis along all the wound extension. Every 10th section was stained with haematoxylin-eosin and was acquired by using a Leica DMR microscope and a high resolution digital camera. The three-dimensional aspect of the lesion was reconstructed with a software system for 3D computer graphics. Microscopic examination and 3D reconstruction demonstrated that the lesion extended to the internal surface of the ventricle wall, although for a very limited extension. 3D reconstruction also showed a certain curvilinear pattern of the lesion in the myocardium, consistent with myocardial contraction at the moment of injury and consequent vitality of the lesion. Moreover, 3D reconstruction permitted to obtain the dimensions of the intra-myocardial injury (corrected for shrinkage and evaluated in the context of muscle contraction), also permitting to furtherly confirm the identification of the knife involved. In conclusion, the present case is indicative of how complete microscopic sectioning and 3D reconstruction may add further information about characteristics of injuries of forensic interest

An anatomo-radiological study of the infrapatellar fat pad

The infrapatellar fat pad (IFP) is an intracapsular, but extrasynovial structure, located between the patellar tendon, femoral condyles and tibial plateau. We have recently described the microscopic orgnisation of the IFP, which consists of white adipose tissue (fibroadipose, lobular type), organised in lobuli delimited by thin connective septa. The aim of the study is to describe the sonoanatomical features of IFP in subjects without knee pathology during flexo-extension movements. Twentyfour volunteers subjects with no history of knee diseases (5M, 19F, mean age: 45yo)were analysed. Examinations was performed using high-resolution grey-scale ultrasound. The mean area of the deepest recognisable adipose chamber in extension were 0,12 and in flexion 0,19 mm2, and the circumference were in extension 1,36 and in flexion 1,19 mm. The area of the closest adipose chamber to the patellar tendon were in extension 0,29 and flexion 0,12 mm2(

Anatomic-radiologic study of the anterolateral ligament of the knee

Recent anatomic investigations of the lateral structures of the knee have identified a new ligament, called the anterolateral ligament (ALL). To date, the anterolateral ligament has not been microscopically analysed. A retrospective MRI study was carried out in 50 patients by two observers. MRI has been performed for various indications excluding trauma. 10 specimens of ALL were sampled from bodies of the Donation to Science program of the University of Padova. A thin linear structure originating at the lateral epicondyle, running obliquely downwards and forwards and inserting at the lateral aspect of the proximal tibia was observed in 18 cases (90%), with a mean length of 3.8 cm and mean thickness of 1.9 mm. The ALL was hyposignal on both T1- and T2-weighted sequences. From the microscopic point of view the ALL corresponds to a fibrous connective tissue, organised in 2-3 layers of collagen tissue (mean thickness 983+423 micron), with scarce elastic fibres, separated by a thin layer of fibroadipose tissue from the adjacent structures. The ALL appears as almost constantly depicted by MRI and shows a fibrous structure. Its layered organisation could account for its mechanical importance, as a presumed stabilizer of the medial rotation of the kne

Hyperoxia-induced changes in morphometric parameters of postnatal neurogenic sites in rat

In literature many works address the effects of hypoxia exposure on postnatal neurogenesis but few data are available about hyperoxia effects, although high oxygen concentrations are frequently used for ventilation of premature newborns. Thus, the aim of the present study was to compare with controls the morphometrical parameters of the main neurogenic sites (subventricular zone and dentate gyrus) in newborn Sprague-Dawley rats exposed to 60% or 95% oxygen for the first 14 postnatal days. Six rats were studied for each of the three groups. The unbiased quantitative method of the optical disector was applied to analyze neuronal densities, nuclear volumes, and total neuron numbers of the subventricular zone and hippocampal dentate gyrus. Apoptosis (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling, TUNEL) and proliferation (Ki67) were also studied. The subventricular zone of newborn rats exposed to 95% hyperoxia showed statistically significant higher volume (mean value ± coefficient of variation: 0.40 ± 0.20 mm3) than subventricular zone of rats raised in normoxia (0.20 ± 0.11 mm3) or 60% hyperoxia (0.26 ± 0.18 mm3). Total neuron number was also significantly higher in 95% hyperoxia while neuronal densities did not reach statistically significant differences. TUNEL showed increased apoptotic indexes in hyperoxic rats. The percentage of proliferating KI67 positive cells was also higher in hyperoxia. The dentate gyrus granular layer of the normoxic rats showed higher volume (0.65 ± 0.11 mm3) than both the hyperoxic groups (60% hyperoxia: 0.39 ± 0.14 mm3; 95% hyperoxia: 0.36 ± 0.16 mm3). Total neuron numbers of hyperoxic dentate gyrus were also significantly reduced; neuronal densities were not modified. Hyperoxia-exposed rats also showed higher apoptotic and proliferating indexes in the dentate gyrus. Hyperoxia exposure in the first postnatal period may affect the main neurogenic areas (subventricular zone and dentate gyrus) increasing apoptosis but also inducing a certain reparative response consisting of increased proliferation. In particular, the increased volume of the subventricular zone may be ascribed to compensatory neurogenic response to the hyperoxic damage. Conversely, the decreased volume of the dentate gyrus granular layer could derive by a non sufficient neurogenic response to counterbalance the hyperoxic neuronal injury

Analisi dell'espressione di spexina e augurina in tessuti di ratto

Analysis of spexin and augurin expression in rat tissuesAnalisi dell'espressione di Spexina e Augurina nei tessuti di ratt

Hyperbaric Air Exposure at 2.5 ATA Does Not Affect Respiratory Mechanics and Lung Histology in the Rat

Abstract BACKGROUND: We previously demonstrated that the exposure to hyperbaric hyperoxia increased respiratory system elastance and both the "ohmic" and viscoelastic components of inspiratory resistances, probably because of increased oxygen tension toxic effects. We presently investigated the possible consequences of a single exposure to 2.5-atmospheres absolute air (hyperbarism) lasting 90 min. METHODS: We used the end-inflation occlusion method on anesthetized rats after about 15 min from previous exposure to hyperbarism. The method allows the measurements of respiratory system elastance and of the ohmic and viscoelastic components of airway resistance, which respectively depend on the Newtonian pressure dissipation due to the ohmic airway resistance to airflow and on the viscoelastic pressure dissipation caused by respiratory system tissue stress relaxation. The expressions of inducible NO synthase (iNOS) and endothelial NO synthase (eNOS) in the lung's tissues were also investigated, together with the histological characteristics of lung tissue. Data were compared with those obtained in control animals and in previously studied animals exposed to hyperoxic hyperbarism. RESULTS: Unlike with hyperoxic hyperbarism, hyperbarism per se did not change significantly the parameters of respiratory mechanics in the control animals (respiratory system elastance and ohmic and viscoelastic resistances were 2.01 \ub1 0.17 vs. 1.74 \ub1 0.08 cm H(2)O/ml, and 0.13 \ub1 0.02 vs. 0.13 \ub1 0.03 and 0.425 \ub1 0.04 vs. 0.33 \ub1 0.03 cm H(2)O/ml s(-1) in control vs. experimental animals, respectively, none significantly different), nor did it induce evident effects on lung histology. An increment of both iNOS and eNOS expressions was documented instead (0.50 \ub1 0.05 vs. 0.75 \ub1 0.07 and 1.04 \ub1 0.1 and 1.4 \ub1 0.15, respectively). CONCLUSION: Our results indicate that, at variance with hyperoxic hyperbarism, the acute exposure to only hyperbarism does not affect either the elastic or the resistive respiratory system properties, or lung histology