Spatial_MvsD

Popn – population ID. Sex.System = M for monoecious, D for dioecious. Lat – latitude in degrees N. Long – longitude in degrees W. Clonal.subrange – mean clonal subrange calculated by GenClone. Ac – Aggregation index (GenClone). P.Ac – P value associated with Ac. Total.Locations – total number of spatial locations surveyed in field. Num.Occupied – number of spatial locations occupied by a ramet. Num.Not.Occupied – number of spatial location not occupied by a ramet. Ppn.Occupied – proportion of spatial positions surveyed occupied by a rame

CloneFlwFreq

SampleNo – original sample number. Popn – population code. Lat – latitude in degrees N. Long – longitude in degrees W. Genotype. Label – clone ID. Clone.Sex. Num.Ramets.Flw - number of ramets associated with multilocus genotype observed flowering (in field). Num.Ramets - number of ramets associated with multilocus genotype. Num.Ramets.NonFlw - number of ramets associated with multilocus genotype not observed flowering (in field). (The following are same as above but NA for genotypes associated with single ramet). Num.Ramets.Flw.1. Num.Ramets.NonFlw.1. Ppn.Ramets.Flw.1. Num.Ramets – number of ramets associated with multilocus genotype. Num.Ramets.Flw – number of ramets associated with multilocus genotype observed flowering (in field). Num.Ramets.NonFlw- number of ramets associated with multilocus genotype not observed flowering (in field). Ppn.Ramets.Flw – proportion of ramets associatied with multilocus genotype observed flowering (in field

CloneSize_byClone_GenoDive_flwfreq

CloneSize_byClone_GenoDive_flwfre

GenoDive

Self extracting stuffit folder of input files formatting for GenoDive. One file for each monoecious population. For each dioecious population there is one file for the fine-scale samples, plus one file (population name + 'f" for flowering) that contains fine-scale plus extra flowering samples. PDFs of genetic distance threshold choice are also in this folder

ClonalDiversity

Popn – population ID. Sex.System. Location – south (s) or north (n). Lat – latitude in degrees N. Long – longitude in degrees W. G – number of multilocus genotypes per population (raw clone assignments). G_corr - number of multilocus genotypes per population (corrected for scoring error/mutations). G_1_corr – (G_corr) minus one. N – number of ramets genotyped. G_N – G divided by N. G_1 – G minus one. N_1 – N minus one. N_1_G_1 – (N minus 1) minus (G minus 1). N_1_G_1_corr – (N_corr minus 1) minus (G_corr minus 1). R_corr - genotypic diversity corrected for scoring error / mutations, R - genotypic diversity (calculated from raw data

CloneArea_MvsD

Input file (for R) for analysis of clone area between monoecious and dioecious populations. Popn – populations ID. Sex.System – M for monoecious, D for dioecious. Lat – latitude in degrees N. Long – latitude in degrees W. Clone.Genotype – genotype ID. Area – area of genotype in metres-squared

CloneSize_FvsM

Input file for R analysis – comparison of clone size between female and male clones in 11 dioecious populations. Each line contains information on a single multilocus genotype. Popn – population ID. Clone.ID. Clone.Sex. Clone.Size.Num – number of ramets assigned to the multilocus genotype. Clone.Size.NumNot – number of ramets assayed not assigned to this multilocus genotype. Total.Clone.Num – total number ramets assayed in population. Clone.Size.Ppn – proportion of ramets assigned to multilocus genotyp

GenCloneFiles

Self-extracting stuff-it folder with GenClone output files for each monoecious and dioecious population (separate folder for each population)

Clonality.Sagittaria.Sexclones

For 11 dioecious populations there is a worksheet with SSR data formatted for GenAlEx; each dioecious populations is named “d popname”. To the right of the raw data worksheet is an output sheet “MS”. On the left is the orginal GenAlEx output – the important column is ‘Label’ which labels samples with identical multilocus genotypes with the same label. To the right the data is sorted by sample ID and the sex phenotype is provided for each sample for which sex phenotype was observed in the field. Below this sex phenotypes (f, h, m) are tallied for calculation of phenotypic sex ratio

Clonality.Sagittaria.IDclones

Raw clone assignments for fine-scale sampling of 10 monoecious and 11 dioecious populations. The raw data for each population is formatted for GenAlEx; dioecious populations are labeled ‘d popname’ and monoecious populations are labeled ‘m popname’. To the right of each raw data worksheet is the GenAlEx output ‘MS’ file. From the raw output calculations of clone size are shown in columns to the right; mean for the population appears at the bottom