Preparation and characterization of cefditoren pivoxil-loaded liposomes for controlled in vitro and in vivo drug release

Gojjala Venugopalarao,1 Rajasekhar Lakshmipathy,2 Nallani Chakravarthula Sarada1 1Environmental and Analytical Chemistry Division, School of Advanced Sciences, VIT University, Vellore, 2Centre for Material Science, KCG College of Technology, Chennai, India Background: The application of antibiotics has been limited due to weak biodistribution and pharmacokinetics. Encapsulation of these drugs in lipid vesicles might be a good solution for obtaining the required properties. Liposomes are one of the most suitable drug-delivery systems to deliver the drug to the target organ and minimize the distribution of the drug to non-target tissues.Objective: The study reported here aimed to develop cefditoren pivoxil liposomes by thin-film hydration, characterize them in terms of physical interactions, and undertake in vitro and in vivo release studies.Methodology: The pre-formulation studies were carried out using Fourier-transform infrared spectroscopy and differential scanning calorimetry. Cefditoren pivoxil liposomal formulations were formulated by thin-film hydration using biomaterials ie, soya lecithin and cholesterol in different molar ratios. The best molar ratio was determined by in vitro studies such as entrapment efficacy, particle size distribution, and diffusion.Results: From the in vitro release studies, it was found that the formulation that contained soya lecithin and cholesterol in a 1.0:0.6 molar ratio gave good entrapment of 72.33% and drug release of 92.5% at 36 hours. Further, the formulation’s zeta potential and surface morphology were examined and stability and in vivo studies were undertaken evaluating the pharmacokinetic parameters, which showed promising results.Conclusion: Formulation CPL VI showed the maximum drug-loading capacity of 72.3% with good controlled release and acceptable stability when compared with the other formulations. In vivo studies in rabbits showed that the drug release from the liposomes was successfully retarded with good controlled release behavior which can be used to treat many bacterial infections with a minimal dose. Keywords: biomaterials, liposomes, drug-delivery system, soya lecithin, cholestero

One-step, low-temperature fabrication of CdS quantum dots by watermelon rind: a green approach

Rajasekhar Lakshmipathy,1 Nallani Chakravarthula Sarada,2 K Chidambaram,3 Sk Khadeer Pasha3 1Centre for Material Science, KCG College of Technology, Chennai, Tamil Nadu, India; 2Environmental and Analytical Chemistry Division, 3Material Physics Division, School of Advanced Sciences, VIT University, Vellore, Tamil Nadu, India Abstract: We investigated the one-step synthesis of CdS nanoparticles via green synthesis that used aqueous extract of watermelon rind as a capping and stabilizing agent. Preliminary phytochemical analysis depicted the presence of carbohydrates which can act as capping and stabilizing agents. Synthesized CdS nanoparticles were characterized using UV-visible, Fourier transform infrared spectroscopy, X-ray diffraction, EDX, dynamic light scattering, transmission electron microscopy, and atomic force microscopy techniques. The CdS nanoparticles were found to be size- and shape-controlled and were stable even after 3 months of synthesis. The results suggest that watermelon rind, an agro-waste, can be used for synthesis of CdS nanoparticles without any addition of stabilizing and capping agents. Keywords: green synthesis, nanoparticles, biomimetic, atomic force microscop