Coprodução de hormônio do crescimento e prolactina em paciente com sela túrcica parcialmente vazia primária: relato de caso

Introduction: The empty sella syndrome is a neuroradiological diagnosis that shows pituitary gland reduction or flattening, with a prevalence of around 8% to 35% in general population. Objective: We report a case in which there is an association between partial primary empty sella syndrome and high levels of growth hormone and prolactin. Methods: A 67-year-old man, from Coronel Mota Hospital, Boa Vista / RR, with no specific complaints, was referred to the Endocrinology ambulatory to investigate a modification in sella area. After anamnesis and physical examination, acromegaly research began. Pituitary hormones and magnetic resonance imaging of the sella turcica with contrast were requested. Results: General physical examination revealed acromegalic face, nasal enlargement, macroglossia and frontal cutis verticis gyrata. Radiological examination showed partial empty sella. Laboratory evaluation confirmed the clinical suspicion of acromegaly and revealed hyperprolactinemia presence. It was decided to treat the patient with cabergoline 1.5 mg / week and to refer him to a center specialized in neuroendocrinology. Conclusion: This report shows a case of partial primary empty sella and co-production of growth hormone and prolactin, making this association interesting, since somatotropinomas are mostly (80%) macroadenomas. In addition, the underdiagnosis of acromegaly is highlighted, since the symptoms are nonspecific and insidious, leading to high morbidity and mortality and reduction of the affected patients life expectancy.Introdução: A síndrome da sela túrcica vazia é um diagnóstico neurorradiológico que evidencia a redução ou o achatamento da glândula pituitária, com prevalência em torno de 8 a 35% na população geral. Objetivo: Este estudo de caso relata a associação entre sela túrcica parcialmente vazia primária e elevados níveis de hormônio do crescimento e prolactina. Métodos: Homem de 67 anos, proveniente do hospital Coronel Mota, Boa Vista/RR, sem queixas específicas, encaminhado ao ambulatório de Endocrinologia para investigação de alteração em região selar. Após realização de anamnese e exame físico, iniciou-se investigação de acromegalia. Foram solicitados basais hipofisários e ressonância magnética de sela túrcica com contraste. Resultados: O exame físico geral evidenciou fácies acromegálica, alargamento nasal, macroglossia e cutis verticis gyrata frontal. O exame radiológico demonstrou sela túrcica parcialmente vazia. A avaliação laboratorial confirmou a suspeita clínica de acromegalia e revelou a presença de hiperprolactinemia. Optou-se por tratar o paciente com cabergolina 1.5mg/semana e encaminhá-lo para centro especializado em neuroendocrinologia. Conclusão: Este relato mostra um caso de sela parcialmente vazia primária e coprodução de hormônio do crescimento e prolactina, tornando esta associação interessante, uma vez que somatotropinomas são em sua maioria (80%) macroadenomas. Além disso, ressalta-se para o subdiagnóstico da acromegalia, uma vez que os sintomas são inespecíficos e insidiosos, acarretando elevada morbimortalidade e redução da expectativa de vida dos pacientes acometidos

Doehlert matrix for the optimization of ultrasound dispersive liquid–liquid microextraction of melatonin in Argentine and Brazilian wine samples

In the present work, a method based on the Ultrasound-dispersive liquid–liquid microextraction (US-DLLME) for the preconcentration of melatonin in Brazilian and Argentinian wine samples prior to the determination by reverse phase high performance liquid chromatography with diode array detector HPLC-DAD was developed. Sample prep optimization was carried out in two stages. First, a full factorial design was executed to evaluate the variables significance. The second step consisted of significant variables optimization (pH, extraction volume, sonication time, centrifugation time) using a Doehlert matrix to determine the critical values. Optimal US-DLLME extraction conditions were as follows: pH of 4.5, sonication time of 6.8 min, centrifugation time of 2.5 min, extractor volume of 250 µL and 5.0 mL of sample. The organic phase was retrieved with a microsyringe to a glass tube, dried and diluted with the mobile phase prior injection. The method presented a limit of detection (LOD) of 0.23 μg L−1 and a limit of quantification (LOQ) of 0.70 μg L−1. The precision expressed as relative standard deviation (R.S.D.) was 0.8% and 1.9% at 10.0 and 1.0 μg L−1, respectively. Accuracy was confirmed by comparison of the results found by the proposed methods with an alternative method, and spike tests with recuperations ranging from 92 to 103%. The analyzed samples showed concentrations varying from 2.2 to 5.0 μg L−1.Fil: Carneiro, Angelica F.. Universidade Federal Do Reconcavo Da Bahia; BrasilFil: Carneiro, Candice N.. Universidade Federal Do Reconcavo Da Bahia; BrasilFil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Spisso, Adrián Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Minho, Lucas A.C.. Universidade Do Estado Da Bahia; BrasilFil: dos Santos, Walter N.L. Universidade Do Estado Da Bahia; BrasilFil: Dias, Fabio de S.. Universidade Federal da Bahia; Brasi