Lipidomic Profile of Rhodotorula toruloides by GC/MS and Antioxidant Capacity of the Oil by DPPH and TLC-Plate Methods

This work was undertaken to evaluate the antioxidant capacity of Rhodotorula toruloides lipid extract in TLC plate, using the (DPPH) (1,1-diphenyl-2-picril-  hydrazine) method as an innovative way to visualise lipid groups that comprise this activity. Similarly, carotenoids and crude oil were analysed for  antioxidant capacity by the DPPH and β-carotene/linoleic acid methods. The lipidomic profile extract analysis was performed by GC/MS and HPLC/DAD.  The sample preparation for the GC/MS analysis was made by ultrasound-assisted transesterification. Free compounds were silylated with BSTFA (N,O-Bis  (trimethylsilyl) trifluoracetamide) + 1% TMCS (Trimethylchlorosilane). The analysis of the lipid extract showed that in the saponifiable fraction saturated  fatty acids (SFA) and monounsaturated fatty acids (MUFA) were present; and in the unsaponifiable fraction were steroids and carotenoids. The antioxidant  capacity was expressed as IC50 reaching 6.4 mg/L that means relative efficiency. The oil profile, using TLC, shows the chemical groups:  carotenoids, acylglycerols, free fatty acids and steroids. Similarly, the GC/ MS analysis shows the fatty acids and steroids. The HPLC analysis describes the  carotenoids profile, highlighting b-carotene as the majority and the presence of β-carotene-5,8-epoxide, zeaxanthin and b-cryptoxanthin, characterising  the lipidomic study of this yeast

Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS

In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work

Levels of As, Cd, Pb and Hg found in the hair from people living in Altamira, Pará, Brazil: environmental implications in the Belo Monte area

Human hair was used as a bioindicator to evaluate the levels of As, Cd, Pb and Hg in the resident population of the "Volta Grande" area of the Xingu river, in the North of Brazil. The concentrations of As and Cd are in agreement with the values found in the literature, however, the Pb and Hg concentration is very different, which can suggest an inter-regional variation. Moreover, the high values found for these elements in the residents of the Canteiro Island can be attributed to environmental exposure of this population in an area with environmental impact from activities of gold mining. The application of these methods showed that the concentrations of the metals studied in this work can correctly classify two different localities in the Altamira district. The results reflect the impact of these elements on the health of the inhabitants of this auriferous area

Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus

Submitted by Sandra Infurna ([email protected]) on 2018-04-10T14:45:44Z No. of bitstreams: 1 mariaines2_sarquis_etal_IOC_2010.pdf: 565543 bytes, checksum: e92d13e1623ceb43ec131b23ef5c5792 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-04-10T14:57:11Z (GMT) No. of bitstreams: 1 mariaines2_sarquis_etal_IOC_2010.pdf: 565543 bytes, checksum: e92d13e1623ceb43ec131b23ef5c5792 (MD5)Made available in DSpace on 2018-04-10T14:57:11Z (GMT). No. of bitstreams: 1 mariaines2_sarquis_etal_IOC_2010.pdf: 565543 bytes, checksum: e92d13e1623ceb43ec131b23ef5c5792 (MD5) Previous issue date: 2010Universidade Federal do Pará. Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos. Belém, PA, Brasil / Universidade Federal do Pará. Programa de Pós-Graduação em Química. Laboratório de Investigação Sistemática em Biotecnologia e Química Fina. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Coleção de Cultura de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Laboratório de Investigação Sistemática em Biotecnologia e Química Fina. Belém, PA, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos. Belém, PA, Brasil / Universidade Federal do Pará. Programa de Pós-Graduação em Química. Laboratório de Investigação Sistemática em Biotecnologia e Química Fina. Belém, PA, Brasil.The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ -pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28◦C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l−1 in 120g l−1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase capable of converting fructose into glucose. In this work, it focused the preference for glucose and, then, of fructose by A. flavus and the strategy used to produce HHMP