Ecophysiological responses of fishes to increasing ocean acidification and warming

A major goal in conservation biology is to understand the effects of short and long term environmental change on organisms. Fishes are the most valuable marine resource, however very little is known about the synergistic effect of current ocean warming and acidification, and the role of body size and local adaptation on their resilience. There is growing evidence that increased environmental temperature correlates with a reduction in ectotherm body size, suggesting a universal response to warming. To investigate the potential advantage of small body size in fish resilience, I made intra- and inter-specific comparisons of dwarf- and normal-size cleaner gobies of the genus Elacatinus. I first tested the hypothesis that smaller body size would correlate with a wider thermal tolerance by using same-age but different-size gobies reared at 'common garden' conditions. By employing critical thermal methodology, I provided empirical evidence supporting thermal biology theories that predict wider thermal tolerance windows as body size shrinks. These results provided the motivation to examine the effect of body mass on digestive performance, an indicator of fitness. Only smaller fish increased digestive metabolic scope at higher temperatures, thus suggesting that temperature increase caused by global warming will favor smaller individuals. To investigate the role of local adaptation on resilience in climate change, I compared the responses to warming and acidification between latitudinally- and morphologically-distinct populations of the little skate Leucoraja erinacea, by focusing on the most vulnerable life stages, embryos and juveniles. Embryos maintained at common garden conditions showed countergradient variation in performance curves. In juvenile skates, post-exercise metabolic curves shifted performance optima, exhibiting thermal adaptation in the two populations examined. This suggests that as skates hatch and are able to thermoregulate, they can change their temperature optima to exploit local thermal environments. Lastly, temperature and acidification levels predicted by the end of the century may reduce fitness of the northern population of skates, thus increasing vulnerability to local extinction

Preparation and in vitro characterization of lipospheres as a carrier for the cosmetic application of glycolic acid

Lipospheres for the cosmetic delivery of glycolic acid were prepared by the melt method using tristearin as the lipid phase and hydrogenated soybean phosphatidylcholine as the emulsifier. The lipospheres, sized from 5 to 40 micron, contained a rather high glycolic acid loading level probably due to a partial polymorphic modification of the lipid and determined glycolic acid sustained release pattern

In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (–)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape-stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG

Resolution of tongue lesions caused by Leishmania infantum in a dog treated with the association miltefosine-allopurinol

Canine leishmaniosis is a severe systemic disease caused by the kinetoplastid protozoan Leishmania infantum, an obligatory intracellular parasite of mammalian macrophages, transmitted by the bite of phlebotomine sandflies. The infection in dogs might occur without any clinical signs or might be characterised by chronic viscerocutaneous signs, such as lymphadenopathy, skin lesions, splenomegaly, onychogryphosis, and renal as well as ocular damage due to immunocomplex deposition. In atypical cases the parasites can be found in the striated musculature, the central nervous system, the endocrine glands or gonads, with or without functional damage. Leishmania infection might seldom induce oral lesions, particularly on the tongue. The authors describe the clinical case of a four-year old mongrel dog with tongue lesions caused by L. infantum. The dog was presented due to diarrhoea, lack of appetite and hypersalivation. Examination of the oral cavity revealed the presence of multiple red, nodular lesions on the dorsal and lateral surfaces of the tongue. Definite diagnosis of an infection with L. infantum was obtained by an indirect immunofluorescence antibody test (IFAT) and by the cytological identification of the parasite in nodular, lingual lesions and bone marrow aspirates. The dog was treated with a combination of miltefosine (Milteforan®, Virbac), 2 mg/kg orally once a day for four weeks and allopurinol (Ziloric®, GlaxoSmithKline), 10 mg/kg orally twice a day for six months. At the end of the treatment, the animal showed full remission of clinical signs. The authors outline the atypical manifestations in the oral cavity in combination with a L. infantum infection and discuss the therapeutic potential of the combination treatment of miltefosine and allopurinol in canine leishmaniosis

Diffuse cutaneous manifestation in a new mother with COVID-19 (SARS-Cov-2)

Our case shows a cutaneous manifestation in a new mother with COVID-19 characterized by the simultaneous presence of erythematous, maculopapular lesions and urticaria-like skin lesions, further highlighting the variety of the clinical features which are associated with this new disease, still little known