23 research outputs found
A Fluorogenic Reagent, 4-Mercapto-7-methylthio-2,1,3-benzoxadiazole for Carboxylic Acids, Designed by Prediction of the Fluorescence Intensity
No full textFluorogenic derivatization reagents suitable for isolation and identification of cysteine-containing proteins utilizing high-performance liquid chromatography-tandem mass spectrometry
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A Photoinduced Electron-Transfer Reagent for Peroxyacetic Acid, 4-Ethylthioacetylamino-7- phenylsulfonyl-2,1,3-benzoxadiazole, Based on the Method for Predicting the Fluorescence Quantum Yields
No full textFluorogenic Derivatization Reagents Suitable for Isolation and Identification of Cysteine-Containing Proteins Utilizing High-Performance Liquid Chromatography−Tandem Mass Spectrometry
No full textControlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method
No full textControlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method
No full textAlthough local protein activity in the cells is one of the hot topics in life science study, it still has many difficulties to measure or change its activity. Here, we prepared 20 nm-sized protein (caspase)-encapsulated photodegradable nanoparticles and used them to control the amount of caspase released within HeLa cells.Encapsulated caspase was released into the cytosol following irradiation. The increased amount of released caspase led to enhanced cell membrane permeability. These results indicate that the amount of protein released within cells can be regulated by the amount of protein-encapsulated nanoparticles; therefore, this technique would be useful for detailed study of protein function within cells or the control of cellular functions
Proton: A Major Factor for the Racemization and the Dehydration at the Cyclization/Cleavage Stage in the Edman Sequencing Method
No full textComplete Enantiomeric Separation of Phenylthiocarbamoylated Amino Acids on a Tandem Column of Reversed and Chiral Stationary Phases
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