Chemical Deposition of Polycrystalline Znse Thin Films from Malonic Acid Solution: Nucleation and Growth Mechanism, Structural, Optical and Electrical Studies

Zinc selenide (ZnSe) polycrystalline semiconductor thin films have been synthesized adopting dip method in malonic acid solution. The formations of nucleus and growth process of this technique have been investigated. The XRD pattern shows a characteristic cubic structure with a (111) crystallographic preferred orientation. SEM studies reveal that grains are uniformly dispersed on the surface of the substrates. The optical energy gap was 2.80 eV. The activation energies are 0.053 and 0.317 eV for low and high temperature region, respectively obtained

Bidirectional modulation of endogenous EpCAM expression to unravel its function in ovarian cancer

<p>Background: The epithelial cell adhesion molecule (EpCAM) is overexpressed on most carcinomas. Dependent on the tumour type, its overexpression is either associated with improved or worse patient survival. For ovarian cancer, however, the role of EpCAM remains unclear.</p><p>Methods: Cell survival of ovarian cancer cell lines was studied after induction or repression of endogenous EpCAM expression using siRNA/cDNA or artificial transcription factors (ATF) consisting of engineered zinc-fingers fused to either a transcriptional activator or repressor domain.</p><p>Results: Two ATFs were selected as the most potent down- and upregulator, showing at least a two-fold alteration of EpCAM protein expression compared with control. Downregulation of EpCAM expression resulted in growth inhibition in breast cancer, but showed no effect on cell growth in ovarian cancer. Induction or further upregulation of EpCAM expression decreased ovarian cancer cell survival.</p><p>Conclusion: The bidirectional ATF-based approach is uniquely suited to study cell-type-specific biological effects of EpCAM expression. Using this approach, the oncogenic function of EpCAM in breast cancer was confirmed. Despite its value as a diagnostic marker and for immunotherapy, EpCAM does not seem to represent a therapeutic target for gene expression silencing in ovarian cancer.</p>