Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form

A simple, economical, precise, and accurate new UV-visible spectrophotometric baseline manipulation method for simultaneous determination of tenofovir disoproxil fumarate (TE) and emtricitabine (EM) in combined tablet dosage form has been developed. e method is based on baseline manipulation (difference) spectroscopy where amplitudes at 261 and 289.9 nm were selected to determine TE and EM, respectively, in combined formulation, and distilled water was used as solvent. Both drugs obey Beer's law in the concentration ranges of 4-20 g/mL for TE and 6-30 g/mL for EM. e results of analysis have been validated statistically, and recovery studies con�rmed the accuracy of the proposed method which was carried out by following the ICH guidelines

Effect of Jyotiṣmatī seed oil on spatial and fear memory using scopolamine induced amnesia in mice

Background: Treatment of memory impairment associated with dementia such as Alzheimer′s disease is still inadequate and requires development of new drugs. Objective: The objective was to evaluate the memory enhancing effect of Celastrus paniculatus seed oil. Materials and Methods: C. paniculatus seed oil was mixed with equal amount of pure ghee and administered orally to mice in the dose of 200 mg/kg/day. Piracetam was used as a standard nootropic. Elevated plus maze and passive avoidance tests were used as a models to test spatial and fear memory respectively. Scopolamine (3 mg/kg, i.p.), was used as an amnestic agent. Results: Mice receiving C. paniculatus showed significant memory enhancement as compared to scopolamine group. The effect of C. paniculatus and combination of C. paniculatus with piracetam was comparable to that with piracetam alone. Conclusion: The present study demonstrates that C. paniculatus seed oil has memory enhancing effect and hence can be developed as a potential drug in the treatment of dementia