Comparative Study of Amino Acids in the Life Stages of Silkworm (Bombyx mori L.)

The silkworm (Bombyx mori L.) undergoes distinct life stages, including egg, larva (caterpillar), pupa (chrysalis), and adult moth(imago), each with specific physiological requirements. Amino acids are essential for the growth and development of the silkworm. In this study, we work in paper chromatography to compare the amino acid profiles in different life stages of the silkworm. Samples were collected from eggs, larvae, pupae, and adult moths. Amino acids were extracted and separated on chromatographic paper using a suitable solvent system. The developed chromatograms were analyzed to determine the relative concentrations of specific amino acids. Our results revealed variations in the amino acid composition across the life stages. The amino acids such as glycine, methionine, leucine, and aspartic acid were common in all life stages of silkworms. These differences may be attributed to the physiological changes associated with metamorphosis and reproduction in adult silkworms. Overall, this study provides valuable insights into the dynamic changes in amino acid profiles during the life stages of silkworms, highlighting the importance of amino acids in their growth, development, and silk production. And, this study demonstrates the utility of paper chromatography as a cost-effective and reliable method for analyzing amino acid composition in complex biological samples, providing valuable insights into the nutritional dynamics of the silkworm lifecycle

GREENHOUSE MONITORING AND CONTROL USING IOT

The population of the world has been increasing gradually in recent times. Under excessive climatic conditions, the plants can be grown with the help of Greenhouse. Green houses help to maintain the temperature within a limited range, used for cultivating plants out of season. The greenhouse control system is powered by ESP-WROOM 32 Wi-Fi Microcontroller and it consists of temperature and humidity sensor, soil moisture sensor, PIR sensor and MQ Gas sensor to monitor and the Incandescent Bulb, air blower and water pump to maintain the environmental conditions. Temperature and humidity sensor senses the level of temperature and moisture and send the bit signal to the controller, which controls the operation of the incandescent lamp to and blower. The moisture content in the soil is sensed with the help of Soil Moisture sensor and it controls the operation of water pump. PIR sensor is used to detect the movement of any organisms such as rodents and send the signal to the buzzer. The Air quality is sensed by the MQ2 Gas Sensor. The data sensed by the sensor is sent to the cloud through Wi-Fi and so that it can be monitored and controlled at any location using the laptop.</jats:p