Isolation and characterisation of a side population from renal CeU carcinoma

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Characterization of the Hoechst 33342 side population from normal and malignant human renal epithelial cells

The fundamental changes which predispose for renal cell carcinoma (RCC) are poorly characterized. It is hypothesized that “cancer stem cells” may be influential in carcinogenesis, and the epithelial side population (SP) is enriched for stemlike cells in other epithelial cancers. In this study, we have isolated and characterized the SP and non-SP (NSP) populations from normal (NK) and malignant (RCC) human kidney tissue. NK specimens were taken from patients undergoing non-renal cancer surgery and paired malignant and macroscopically normal tissue samples were taken from patients undergoing surgery for RCC. The Hoechst 33342 dye efflux technique was used to isolate epithelial SP and NSP from normal and malignant human renal tissue. Cellular subpopulations were phenotyped for lineage, cell cycle, and putative stem cell markers, and functionally characterized using in vitro colony-forming and proliferation assays. The SP constituted 3.8 ± 0.4 and 5.9 ± 0.9% of epithelial cells in NK and RCC, respectively, of which 14.1 ± 3.5 and 13.2 ± 3.6% were shown to be in G0. SP cells demonstrated greater proliferative potential in colony-forming efficiency, long-term culture, and spheroids assays and were shown to be maintained upon tissue culture passage. We have shown that the renal SP is enriched for quiescent cells, with a high proliferative capacity and stemlike properties. The population is, however, heterogeneous, confirming that the terms “SP cell” and “stem cell” cannot be used interchangeably

Phytotherapy in Chronic Prostatitis

Chronic prostatitis/chronic pelvic pain syndrome continues to pose a treatment challenge for urologists. Chronic prostatitis is a very common and poorly understood condition with significant impact on quality of life. In recent literature, studies have been conducted with various treatment modalities that include antibiotics, α-blockers, anti-inflammatory agents, and cognitive behavioral interventions such as biofeedback and psychotherapy. Patients have shown interest in phytotherapy as a treatment option with increasing frequency due to lack of efficacy of conventional therapies. However, very little is known about the efficacy of second- and third-line treatments, such as the use of herbal supplements. We review published literature regarding phytotherapy usage for chronic prostatitis. The treatments include Chinese herbs, green tea extract, zinc, cernitin pollen extract (bee pollen), quercetin, saw palmetto (Serenoa repens), and lycopene. © 2009 Current Medicine Group LLC.SCOPUS: re.jinfo:eu-repo/semantics/publishe

Localising prostate cancer: comparison of endorectal magnetic resonance (MR) imaging and 3D-MR spectroscopic imaging with transrectal ultrasound-guided biopsy.

Magnetic resonance imaging (MRI) and MR spectroscopic imaging (MRSI) have been gaining acceptance as tools in the evaluation of prostate cancer. We compared the accuracy of transrectal ultrasound (TRUS)-guided biopsy and dynamic contrast-enhanced MRI combined with three-dimensional (3D) MRSI in locating prostate tumours and determined the influence of prostate weight on MRI accuracy.Comparative StudyJournal ArticleSCOPUS: re.jinfo:eu-repo/semantics/publishe