COMPUTATIONAL INTERACTION OF ENTOMOPATHOGENIC FUNGAL SECONDARY METABOLITES WITH PROTEINS INVOLVED IN HUMAN XENOBIOTIC DETOXIFICATION

Objective: Entomopathogenic fungi are rich source of secondary metabolites which posses both pharmacological and insecticidal activity. It is essential to assess metabolite toxicity of chemically diverse toxic metabolites of entomopathogenic fungus. Human acetylcholine esterase, cytochrome p450 and glutathione S-transferase are important enzymes involved in human xenobiotic detoxification. Methods: In this study, in silico interaction of 13 selected secondary metabolites of entomopathogenic fungi with the target human proteins were carried out using Molegro Virtual Docker 4.0.2. Results: This study reveals serinocyclin-A, have shown highest binding energy (176.07 KJ mol-1) with glutathione S-transferase followed by helvolic acid, cytochalasin B and beauverolide H have shown considerable inhibition among the metabolites tested. Conclusion: The study concludes that serinocyclin-A, helvonic acid, cytochalasin B and beauverolide among 13 secondary metabolites tested were found to be more toxic and may inhibit the human metabolic pathways

COMPATIBILITY OF BEAUVERIA BASSIANA (BALS.) VUILL ISOLATES WITH SELECTED INSECTICIDES AND FUNGICIDES AT AGRICULTURE SPRAY TANK DOSE

The effects of six commonly used chemical pesticides in which three are insecticides namely quinolphos, monocrotophos, cypermethrin and three fungicides carbendazim, cooper oxychloride and mancozeb on germination, mycelial growth of thirty isolates of Beauveria bassiana was studied. Except mancozeb all the insecticides and fungicides doesn't show a significant inhibition effect on the germination of conidia. There was significant inhibition of mycelia growth in few isolates with insecticides as well as fungicides except copper oxychloride. Copper oxychloride promoted the mycelia growth in many isolates where as mancozeb inhibited the mycelia growth of all the isolates tested in the present study. This work suggests that the most appropriate insecticide and fungicides for use in Integrated Pest Management Programs in combination with Beauveria bassiana isolates. It is important to test the compatibility of isolates of entomopathogenic fungi with the pesticides commonly used in IPM programmes.Â