1,505 research outputs found
Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers
<p>Abstract</p> <p>Background</p> <p>DNA barcoding uses a 650 bp segment of the mitochondrial cytochrome <it>c </it>oxidase I (COI) gene as the basis for an identification system for members of the animal kingdom and some other groups of eukaryotes. PCR amplification of the barcode region is a key step in the analytical chain, but it sometimes fails because of a lack of homology between the standard primer sets and target DNA.</p> <p>Results</p> <p>Two forward PCR primers were developed following analysis of all known arthropod mitochondrial genome arrangements and sequence alignment of the tRNA-W gene which was usually located within 200 bp upstream of the COI gene. These two primers were combined with a standard reverse primer (LepR1) to produce a cocktail which generated a barcode amplicon from 125 of 141 species that included representatives of 121 different families of Hexapoda. High quality sequences were recovered from 79% of the species including groups, such as scale insects, that invariably fail to amplify with standard primers.</p> <p>Conclusions</p> <p>A cocktail of two tRNA-W forward primers coupled with a standard reverse primer amplifies COI for most hexapods, allowing characterization of the standard barcode primer binding region in COI 5' as well as the barcode segment. The current results show that primers designed to bind to highly conserved gene regions upstream of COI will aid the amplification of this gene region in species where standard primers fail and provide valuable information to design a primer for problem groups.</p
Query-Efficient Black-Box Red Teaming via Bayesian Optimization
The deployment of large-scale generative models is often restricted by their
potential risk of causing harm to users in unpredictable ways. We focus on the
problem of black-box red teaming, where a red team generates test cases and
interacts with the victim model to discover a diverse set of failures with
limited query access. Existing red teaming methods construct test cases based
on human supervision or language model (LM) and query all test cases in a
brute-force manner without incorporating any information from past evaluations,
resulting in a prohibitively large number of queries. To this end, we propose
Bayesian red teaming (BRT), novel query-efficient black-box red teaming methods
based on Bayesian optimization, which iteratively identify diverse positive
test cases leading to model failures by utilizing the pre-defined user input
pool and the past evaluations. Experimental results on various user input pools
demonstrate that our method consistently finds a significantly larger number of
diverse positive test cases under the limited query budget than the baseline
methods. The source code is available at
https://github.com/snu-mllab/Bayesian-Red-Teaming.Comment: ACL 2023 Long Paper - Main Conferenc
Controlling Citrate Synthase Expression by CRISPR/Cas9 Genome Editing for n-Butanol Production in Escherichia coli
Two-dimensional TiO2 inverse opal with a closed top surface structure for enhanced light extraction from polymer light-emitting diodes
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