Identification of molecular integrators shows that nitrogen activelycontrolsthephosphatestarvationresponseinplants

Nitrogen (N) and phosphorus (P) are key macronutrients sustaining plant growth and crop yield and ensuring food security worldwide. Understanding how plants perceive and interpret the combinatorial nature of these signals thus has important agricultural implications within the context of (1) increased food demand, (2) limited P supply, and (3) environmental pollution due to N fertilizer usage. Here, we report the discovery of an active control of P starvation response (PSR) by a combination of local and long-distance N signaling pathways in plants. We show that, in Arabidopsis (Arabidopsis thaliana), the nitrate transceptor CHLORINA1/NITRATE TRANSPORTER1.1 (CHL1/NRT1.1) is a component of this signaling crosstalk. We also demonstrate that this crosstalk is dependent on the control of the accumulation and turnover by N of the transcription factor PHOSPHATE STARVATION RESPONSE1 (PHR1), a master regulator of P sensing and signaling. We further show an important role of PHOSPHATE2 (PHO2) as an integrator of the N availability into the PSR since the effect of N on PSR is strongly affected in pho2 mutants. We finally show that PHO2 and NRT1.1 influence each other’s transcript levels. These observations are summarized in a model representing a framework with several entry points where N signal influence PSR. Finally, we demonstrate that this phenomenon is conserved in rice (Oryza sativa) and wheat (Triticum aestivum), opening biotechnological perspectives in crop plants.This work was supported in the Honude group (Biochemistry & Plant Molecular Physiology) by Agence Nationale de la Recherche (IMANA ANR-14-CE19-0008 with a doctoral fellowship to A.S.), by the Centre National de la Recherche Scientifique (CNRS LIA-CoopNet to G.K.), and by the National Science Foundation (NSF IOS 1339362-NutriNet). Research in V.R.’s laboratory was funded by the Ministry of Economy and Competitiveness and AEI/FEDER/European (grants BIO2013-46539-R and BIO2016-80551-R)

Nutrient-related Long-Distance Signals: common players and possible crosstalk.

Nutrient fluctuations are more a rule rather than an exception in the life of sessile organisms such as plants. Despite this constraint that adds up to abiotic and biotic stresses, plants are able to accomplish their life cycle thanks to an efficient signaling network that reciprocally control nutrient acquisition and use with growth and development. The majority of nutrients are acquired by the root system where multiple local signaling that rely on nutrient sensing systems are implemented to direct root growth toward soil resources. Moreover, long-distance signaling plays an essential role in integrating nutrient availability at the whole plant level and adjusting nutrient acquisition to plant growth requirements. By studying the signaling network for single mineral nutrients, several long-distance signals traveling between roots and shoots and taking a diversity of forms have been identified and are summarized here. However, the nutritional environment is multifactorial, adding a tremendous complexity for our understanding of the nutrient signaling network as a unique system. For instance, long-distance signals are expected to in part support this nutrient crosstalk but the mechanisms are is still largely unknown. Therefore, the involvement of possible long-distance signals as conveyers of nutrient crosstalk is discussed here together with approaches and strategies that are now considered to build a picture from the nutrient-signaling puzzle

Regulatory Network Behind Systemic Nitrogen Signaling, in Arabidopsis

Regulatory Network Behind Systemic Nitrogen Signaling, in Arabidopsis. Gordon Research Conference :Plant Molecular Biology "Dynamic Plant Systems

Regulatory Network behind Systemic Nitrogen Signaling, in Arabidopsis

International audienc

Regulatory Network Behind Systemic Nitrogen Signaling, in Arabidopsis

Regulatory Network Behind Systemic Nitrogen Signaling, in Arabidopsis. Gordon Research Conference :Plant Molecular Biology "Dynamic Plant Systems

Résistances récessives aux potyvirus chez les solanacées et facteurs du complexe d'initiation de la traduction

Dir. de thèse: Carole Caranta *INRA Centre d'Avignon, Documentation, Site Agroparc 84914 Avignon Cedex9 Diffusion du document : INRA Centre d'Avignon, Documentation, Site Agroparc 84914 Avignon Cedex9 Diplôme : Dr. d'Universit

Systemic nutrient signalling: On the road for nitrate.

Whole plant nutrient signalling involves bidirectional exchange of signal molecules between roots and shoots. For nitrogen uptake, in addition to the root-to-shoot delivery of nitrogen-deprivation information, a shoot-to-root path is now defined