Receiver Operating Characteristic (ROC) curves showing the association of the <i>PIK3CA</i>-GS with <i>PIK3CA</i> mutation status. A.

<p>Dataset A, n = 22/58 (38.5%) AUC = 0.67, 95%CI: 0.5–0.8, p = 0.04), <b>B.</b> Dataset B n = 6/23 (26%) AUC 0.77, 95%CI : 0.6–0.96, p =  = 0.059). <b>C</b> and <b>D</b> show the individual samples for each dataset and distinguish between sequenced kinase (exon 20- squares) and helical (exon 9-circles) mutations and wild-type (WT-black) <i>PIK3CA</i>.</p

Baseline characteristics of the two datasets used in this analysis.

<p>The second column compares the dataset A with the original previously published global trial population.</p><p># as previously published in Baselga et al, JCO 2009 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053292#pone.0053292-Dowsett1" target="_blank">[11]</a> (original cohort of which the Dataset A is a subset); Dataset B published by Sabine et al, BCR 2010 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053292#pone.0053292-Ellis2" target="_blank">[13]</a>.</p>**<p>As previously defined in Dowsett et al (CCR 2007) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053292#pone.0053292-Kalinsky1" target="_blank">[9]</a>;</p

Relative change in %Ki67 from baseline to day 15 by treatment arm.

<p>According <i>PIK3CA</i> genotype in <b>A</b> dataset A; B dataset <b>B</b> According to <i>PIK3CA</i>-GS scores by treatment arm: regression lines for relative change in %Ki67 from baseline to day 15 <b>C</b> dataset A; <b>D</b> dataset B <b>E</b> Relative change in %Ki67 from baseline to day 15 by treatment arm and according to increasing tertiles of the <i>PIK3CA</i>-GS in dataset A; <b>F</b> Number of Absolute D15 responders by treatment arm and according to increasing tertiles of the <i>PIK3CA</i>-GS in dataset A.</p

Associations between PIK3CA-GS and relative change in phosphorylated S6. A

<p>Dataset B: relative percentage change in pS6 (S240) as measured by IHC from baseline to day 15 according <i>PIK3CA</i> genotype <b>B</b> Regression line for relative change in pS6 from baseline to day 15 according to <i>PIK3CA</i>-GS in dataset B <b>C</b> Dataset A: Relative percentage change in phosphorylated S6 (S240) as measured by IHC from baseline to day 15 according <i>PIK3CA</i> genotype and treatment arm.</p

Regression lines for relative change in %Ki67 from baseline to day 15 according to <i>PIK3CA</i>-GS scores by treatment arm by <i>PIK3CA</i> genotype in dataset A. A

<p>PIK3CA WT; <b>B</b> PIK3CA mutant Relative change in %Ki67 from baseline to day 15 by treatment arm and according to increasing tertiles of the <i>PIK3CA</i>-GS by <i>PIK3CA</i> genotype in dataset A <b>C</b> PIK3CA WT; <b>D</b> PIK3CA mutant.</p