From microbial sprays to insect-resistant transgenic plants: history of the biospesticide Bacillus thuringiensis. A review

Bacillus thuringiensis, known as Bt, is a spore-forming bacterium that occurs naturally in soil and that produces highly specific insecticidal proteins called Cry proteins. These proteins are stomach poisons that specifically affect insects. Today, Bt preparations are considered as the most effective, specific and environmentally-friendly bioinsecticides; they have been used as biological pesticides in agriculture, forestry and in human health for the elimination of vectors of diseases for more than 60 years and their implementation far exceeds other microbial agents such as fungi, protozoa or viruses. This review on the use of this entomopathogenic bacterium in crop protection is not intended to be a compilation of the results of all the investigations made in this field. Instead, it is an attempt to provide an overview of the major trends and developments of Bt for the control of agricultural insect pests and to describe the main approaches that have been used to improve this natural bioinsecticide. Bt-based insecticides are considered safe for mammals and birds, and are safer for non-target insects than conventional insecticides; they have become the most widely used microbial insecticides. However, Bt products have several limitations, such as a narrow activity spectrum, instability in rain and sunlight, and inefficiency against pest feeding on internal tissues of the plants. The first step towards improving Bt has involved the isolation of new strains with higher and broader insecticidal activity against targeted insect pests and the cloning of cry genes encoding new insecticidal crystal proteins. A second strategy was to increase the persistence of its toxins in the field by encapsulation in recombinant asporogenic Bt strains or other heterologous recombinant microbial hosts; this protected the toxins against UV degradation and had the advantage that the transgenic microorganisms released into the environment were non-viable. Bt has also become a key source of genes for transgenic expression to provide pest resistance in plants and in so-called genetically modified plants. The engineering of plants to express Bt cry genes has been especially helpful against pests that attack parts of the plant that are usually not well protected by conventional insecticide application. The potential effects on human health and the environment of the large-scale use of these Bt crops are also in the scope of this revie

Three-particle correlations in QCD jets and beyond

In this paper, we present a more detailed version of our previous work for three-particle correlations in quark and gluon jets [1]. We give theoretical results for this observable in the double logarithmic approximation and the modified leading logarithmic approximation. In both resummation schemes, we use the formalism of the generating functional and solve the evolution equations analytically from the steepest descent evaluation of the one-particle distribution. In addition, in this paper we include predictions beyond the limiting spectrum approximation and study this observable near the hump of the single inclusive distribution. We thus provide a further test of the local parton hadron duality (LPHD) and make predictions for the LHC. The computation of higher rank correlators is presented in the double logarithmic approximation and shown to be rather cumbersome.Comment: 34 pages and 14 figure

Three-particle correlations in QCD parton showers

Three-particle correlations in quark and gluon jets are computed for the first time in perturbative QCD. We give results in the double logarithmic approximation and the modified leading logarithmic approximation. In both resummation schemes, we use the formalism of the generating functional and solve the evolution equations analytically from the steepest descent evaluation of the one-particle distribution. We thus provide a further test of the local parton hadron duality and make predictions for the LHC.Comment: 9 pages and 5 figures. Version published by Physical Review D with reference: Phys. Rev. D 84, 034015 (2011). Two more figures and one section adde

Pathogenic A. salmonicida from human patients

The bacterium Aeromonas salmonicida is known since long time as a major fish pathogen unable to grow at 37 °C. However, some cases of human infection by putative mesophilic A. salmonicida have been reported. The goal of the present study is to examine two clinical cases of human infection by A. salmonicida in Spain and to investigate the pathogenicity in mammals of selected mesophilic A. salmonicida strains. An evaluation of the pathogenicity in a mouse model of clinical and environmental A. salmonicida strains was performed. The genomes of the strains were sequenced and analyzed in order to find the virulence determinants of these strains. The experimental infection in mice showed a gradient in the virulence of these strains and that some of them can cause necrotizing fasciitis and tissue damage in the liver. In addition to demonstrating significant genomic diversity among the strains studied, bioinformatics analyses permitted also to shed light on crucial elements for the virulence of the strains, like the presence of a type III secretion system in the one that caused the highest mortality in the experimental infection. Clinicians and microbiologists should consider these results for the inclusion of A. salmonicida in diagnosis tests since it is now clear that some mesophilic strains are also pathogens for humans

Heavy quark flavour dependence of multiparticle production in QCD jets

After inserting the heavy quark mass dependence into QCD partonic evolution equations, we determine the mean charged hadron multiplicity and second multiplicity correlators of jets produced in high energy collisions. We thereby extend the so-called dead cone effect to the phenomenology of multiparticle production in QCD jets and find that the average multiplicity of heavy-quark initiated jets decreases significantly as compared to the massless case, even taking into account the weak decay products of the leading primary quark. We emphasize the relevance of our study as a complementary check of $b$-tagging techniques at hadron colliders like the Tevatron and the LHC.Comment: Version revised, accepted for publication in JHEP, 21 pages and 7 figure

The K2-ESPRINT Project III: A Close-in Super-Earth around a Metal-rich Mid-M Dwarf

We validate a $R_p=2.32\pm 0.24R_\oplus$ planet on a close-in orbit ($P=2.260455\pm 0.000041$ days) around K2-28 (EPIC 206318379), a metal-rich M4-type dwarf in the Campaign 3 field of the K2 mission. Our follow-up observations included multi-band transit observations from the optical to the near infrared, low-resolution spectroscopy, and high-resolution adaptive-optics (AO) imaging. We perform a global fit to all the observed transits using a Gaussian process-based method and show that the transit depths in all passbands adopted for the ground-based transit follow-ups ($r'_2, z_\mathrm{s,2}, J, H, K_\mathrm{s}$) are within $\sim 2\sigma$ of the K2 value. Based on a model of the background stellar population and the absence of nearby sources in our AO imaging, we estimate the probability that a background eclipsing binary could cause a false positive to be $< 2\times 10^{-5}$. We also show that K2-28 cannot have a physically associated companion of stellar type later than M4, based on the measurement of almost identical transit depths in multiple passbands. There is a low probability for a M4 dwarf companion ($\approx 0.072_{-0.04}^{+0.02}$), but even if this were the case, the size of K2-28b falls within the planetary regime. K2-28b has the same radius (within $1\sigma$) and experiences a similar irradiation from its host star as the well-studied GJ~1214b. Given the relative brightness of K2-28 in the near infrared ($m_\mathrm{Kep}=14.85$ mag and $m_H=11.03$ mag) and relatively deep transit ($0.6-0.7\%$), a comparison between the atmospheric properties of these two planets with future observations would be especially interesting.Comment: 11 pages, 9 figures, accepted to Ap

PEPTIDES EXTRACTED FROM ARTEMISIA HERBA ALBA HAVE ANTIMICROBIAL ACTIVITY AGAINST FOODBORNE PATHOGENIC GRAM-POSITIVE BACTERIA

Background: Artemisia herba alba, classified into the family of Asteraceae, is an aromatic herb that is traditionally used as a purgative and anti-pyretic folk medicine by rural people of south Tunisia. This study reports the first identification of antimicrobial peptides from this medicinal plant that inhibited the growth of several food-borne pathogenic bacteria. Materials and methods: The extraction and purification of peptidic agents from Artemisia herba alba, have been performed using precipitation by ammonium sulfate of a phosphate buffer crude extract obtained from the plant leaves, followed by reverse-phase HPLC on a C18 column. The mass of the peptides was estimated by SDS-PAGE electrophoresis, followed by a gel overlay assay and ultra-filtration through a 5 kDa cut-off membrane. Fractions from every purification steps were sampled and assayed for activity towards different food-borne bacterial strains pathogenic and non pathogenic to humans. Results: The phosphate buffer crude extract, as well as its ammonium sulfate precipitate, designated AS-P, inhibited the growth of Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus sensu stricto and the new approved species Bacillus cytotoxicus. AS-P MICs (minimum inhibitory concentrations) ranged from 0.241 to 3.8 mg/ml proteins for L. monocytogenes and B. cereus sensu stricto (strains ATCC10987 and IP5832), respectively. The bioactive AS-P molecules were stable up to 10 minutes heating at 120°C and they resisted organic solvent effects. Antimicrobial activity of A. herba alba AS-P decreased to 40 and 60% after proteolytic treatment with trypsin and proteinase K, respectively, suggesting peptides being responsible for the A. herba alba AS-P activity. The mass of antibacterial A. herba alba peptides was estimated below 5 kDa. Two AS-P fractions, eluted at 40 and 37% acetonitrile, showed antibacterial activity when assayed against L. monocytogenes. Conclusion: A. herba alba could make a new source of novel natural anti-infective agents that could be used in food bio-preservation as natural additives or in human infectious disease treatments against multi-drug resistant pathogens

The K2-ESPRINT Project II: Spectroscopic follow-up of three exoplanet systems from Campaign 1 of K2

We report on Doppler observations of three transiting planet candidates that were detected during Campaign 1 of the K2 mission. The Doppler observations were conducted with FIES, HARPS-N, and HARPS. We measure the mass of EPIC 201546283b, and provide constraints and upper limits for EPIC 201295312b and EPIC 201577035b. EPIC 201546283b is a warm Neptune orbiting its host star in 6.77 days and has a radius of 4.45_(-0.33)^(+0.33)R_⊕ and a mass of 29.1_(-7.4)^(+7.5)M_⊕, which leads to a mean density of 1.80_(-0.55)^(+0.70) cm^(-3). EPIC 201295312b is smaller than Neptune with an orbital period of 5.66 days, a radius of 2.75_(-0.22^)(0.24)R_⊕, and we constrain the mass to be below 12 M_⊕ at 95% confidence. We also find a long-term trend indicative of another body in the system. EPIC 201577035b, which was previously confirmed as the planet K2-10b, is smaller than Neptune, orbiting its host star in 19.3 days, with a radius of 3.84_(-0.34)^(+0.35)R_⊕. We determine its mass to be 27_(-16)^(+17)M_⊕, with a 95% confidence upper limit at 57M_⊕, and a mean density of 2.6_(-1.6)^(+2.1)g cm^(-3). These measurements join the relatively small collection of planets smaller than Neptune with measurements or constraints of the mean density. Our code for performing K2 photometry and detecting planetary transits is now publicly available