Effect of Cholesterol and Different Solvents on Particle Size, Zeta Potential and Drug Release of Eucalyptus Oil Phytosome

Background: Herbal extracts show poor absorption and bioavailability but their complex with phospholipids i.e., phytosome improves this major problem. Aim: The aim of research was to examine the effect of cholesterol and different organic solvents on particle size and zeta potential of eucalyptus oil phytosome. Materials and Methods: Totally six batches of phytosomes were prepared using three solvents chloroform, dichloromethane, acetone, with and without addition of cholesterol. Phytosome were evaluated for yield, FTIR, partial size and zeta potential, drug entrapment and drug release, drug release kinetics and stability studies. Results: The yield, density refractive index and maximum absorbance (λmax) of eucalyptus oil was found to be 1.65±0.74%, 0.9928 g/cm³ (24.2°C), 1.3613(24.6°C), and 297.733 nm respectively. There was no drug excipient interaction. The yield varied from 85.14±0.74 to 87.14±0.74%, particle size varied from 71.76±0.63 to 197.36±0.53 nm, zeta potential varied from 15.3±0.27 to-28.2±0.26 mV and entrapment efficiency varied from 57.45±0.35 to 67.34±0.52 respectively. The in vitro drug release varied from 87.26±0.63 to 71.35±0.63% up to 300 min (5 hr) and batch-A was selected as best formulation that showed Peppas Korsmeyer as the best fit model with R2 value 0.9422 and mechanism of drug release was Fickian Diffusion (Higuchi Matrix). The stability studies showed 99.14±0.25 to 99.55±0.52% drug content on 28th day. Conclusion: The particle size of phytosomes was increased on addition of cholesterol. The acetone showed the smallest particle size and chloroform showed the biggest particle size that indicated that molecule diameter and molecular weight affects the solubility and assembly of phospholipid/ cholesterol in solvent in order to prepare particulate drug delivery syste

Determination of Yield and Yield Characteristics of Some Barley (Hordeum vulgare L.) Genotypes Under Supplemental Irrigation Conditions in Van

This research was carried out to investigate the adaptability abilities of some barley varieties in Van ecological conditions in the winter growing season of 2020-2021. Sixteen genotypes were grown according to randomized block design with three replications. The quantity of supplemental irrigation was equivalent to a total precipitation of 114 mm. According to the results obtained, the number of days to spike was 167.67-175.66 days, plant height 63.37-91.85 cm, number of spikes per square meter 403.67-619.67, lodging 0-10%, chlorophyll ​​​​45.34- 53.09 SPAD units, maturity period 36.0-36.7 days, canopy temperature 30.0-30.4 oC, spike length 7.30-9.96 cm, number of spikelets per spike 33.8-21.7, number of fertile spikelets 7.13-15.73, thousand grain weight 36.67-45.83 g, hectolitre weight 62.30 – 66.10 kg/hl, biological yield ​​20.36-20.45 t/ha, and grain yield 3.08–6.98 t/ha. The highest grain yield was obtained from two row barley varieties; Calabey, Akar, Tosunpasa and Asil. The highest biological yield was measured in Anka-11 and Aydanhanim cultivars

Genetic Variation of Flowering Trait in Maize (Zea mays L.) under Drought Stress at Vegetative and Reproductive Stages

A study was conducted under water stress in vegetative and reproductive growth stages at two locations (Shambat and Medani) in Sudan during the season (2003/2004). Genotypic and phenotypic variability, genetic advance and heritability in a broad sense were estimated in a split-plot layout within randomized complete block design with three replications. Fifteen genotypes of maize were used for the study.  Phenotypic correlation coefficient between grain yield and 25%, 50% and 95% of flowering trait anthesis and silking were evaluated. All flowering stages were found significant differences among the genotypes, except days to 95% anthesis. Significant differences among water treatments were observed for days to 25% silking at Medani. High heritability, genotypic coefficient of variation and genetic advance were exhibited by days to 25% silking. Grain yield was significantly and negatively associated with days to (50% and 95%) silking. Based on the results drought stress at vegetative and reproductive stages of maize results in a drastic reduction in grain yield, and flowering character would be the important selection, creation for maize improvement under drought stress. Keywords: Maize (Zea mays L.), Genetic variability, heritability, correlation, flowering, drought

An Ovarian Bioreactor for In Vitro Culture of the Whole Bovine Ovary: a Preliminary Report

Background: Improved cancer therapeutics and enhanced cancer survivorship have emphasized the severe long-term side effects of chemotherapy. Specifically, studies have linked many chemotherapy agents with primary ovarian insufficiency, although an exact insult model has not yet been determined. To investigate and ultimately solve this problem, a novel device for extended study of mammalian ovaries in vitro was developed. Methods: A bioreactor was fabricated for bovine ovarian culture that provides intravascular delivery of media to the ovary through isolation and cannulation of a main ovarian artery branch. Whole ovaries were cultured in vitro using three methods: (1) continuously supplied fresh culture media, (2) recirculated culture media, or (3) continuously supplied fresh culture media supplemented with 500 nM doxorubicin for 24 or 48 h. TUNEL assay was used to assess apoptotic cell percentages in the three groups as compared to uncultured baseline ovaries. Results: The ovary culture method was shown to maintain cell viability by effectively delivering nutrient-enriched pH-balanced media at a constant flow rate. Lower apoptosis observed in ovaries cultured in continuously supplied fresh culture media illustrates that this culture device and method are the first to sustain whole bovine ovary viability for 48 h. Meanwhile, the increase in the percentage of cell apoptosis with doxorubicin treatment indicates that the device can provide an alternative model for testing chemotherapy and chemoprotection treatments to prevent primary ovarian insufficiency in cancer patients. Conclusions: An ovarian bioreactor with consistent culture media flow through an ovarian vasculature-assisted approach maintains short-term whole bovine ovary viability

An Ovarian Bioreactor for In Vitro Culture of the Whole Bovine Ovary: a Preliminary Report

Background: Improved cancer therapeutics and enhanced cancer survivorship have emphasized the severe long-term side effects of chemotherapy. Specifically, studies have linked many chemotherapy agents with primary ovarian insufficiency, although an exact insult model has not yet been determined. To investigate and ultimately solve this problem, a novel device for extended study of mammalian ovaries in vitro was developed. Methods: A bioreactor was fabricated for bovine ovarian culture that provides intravascular delivery of media to the ovary through isolation and cannulation of a main ovarian artery branch. Whole ovaries were cultured in vitro using three methods: (1) continuously supplied fresh culture media, (2) recirculated culture media, or (3) continuously supplied fresh culture media supplemented with 500 nM doxorubicin for 24 or 48 h. TUNEL assay was used to assess apoptotic cell percentages in the three groups as compared to uncultured baseline ovaries. Results: The ovary culture method was shown to maintain cell viability by effectively delivering nutrient-enriched pH-balanced media at a constant flow rate. Lower apoptosis observed in ovaries cultured in continuously supplied fresh culture media illustrates that this culture device and method are the first to sustain whole bovine ovary viability for 48 h. Meanwhile, the increase in the percentage of cell apoptosis with doxorubicin treatment indicates that the device can provide an alternative model for testing chemotherapy and chemoprotection treatments to prevent primary ovarian insufficiency in cancer patients. Conclusions: An ovarian bioreactor with consistent culture media flow through an ovarian vasculature-assisted approach maintains short-term whole bovine ovary viability

Phenotypic Variations of Drought Tolerance Parameters in Maize (Zea mays L.) under Water Stress at Vegetative and Reproductive Stages

Two field experiments were conducted at Shambat and Medani in Sudan under water stress in vegetative and reproductive growth stages during the season (2003/2004). The objective of the study was to assess phenotypic variability and phenotypic correlation between drought tolerance parameters in maize genotypes. A split-plot layout within randomized complete block design with three replications was used for the experiment. Fifteen genotypes of maize were examined through the study. The effect due to genotypes x location was highly significant for all drought tolerance parameters studied. Highly significant and favorable correlation for Yw with SSI2, SSI3, GMP2, GMP3, STI2 and STI3, while highly significant and negative with Yd2/Yw and Yd3/Yw. Based on the result's drought stress at vegetative and reproductive stages of maize results in a drastic reduction in grain yield, and the strong positive correlation of Yw with SSI, GMP and STI, indicates that selection for high values of these parameters improves yield under stress and non-stress environments. Drought tolerance parameters can be used for improving grain yield and facilitate further efforts in maize breeding programs. Keywords: Correlation, drought, genetic variability, heritability, Maize (Zea mays L.).

The Effect of Tamoxifen and Raloxifene on Estrogen Metabolism and Endometrial Cancer Risk

Selective estrogen receptor modulators (SERMs) demonstrate differential endometrial cancer (EC) risk. While tamoxifen (TAM) use increases the risk of endometrial hyperplasia and malignancy, raloxifene (RAL) has neutral effects on the uterus. How TAM increases the risk of EC and why TAM and RAL differentially modulate the risk for EC, however, remain elusive. Here, we tested the hypothesis that TAM increases the risk for EC, at least in part, by enhancing the local estrogen biosynthesis and directing estrogen metabolism towards the formation of genotoxic and hormonally active estrogen metabolites. In addition, the differential effects of TAM and RAL in EC risk are attributed to their differential effect on estrogen metabolism/metabolites. The endometrial cancer cell line (Ishikawa cells) and the nonmalignant immortalized human endometrial glandular cell line (EM1) were used for the study. The profile of estrogen/estrogen metabolites (EM), depurinating estrogen-DNA adducts, and the expression of estrogen-metabolizing enzymes in cells treated with 17β-estradiol (E2) alone or in combination with TAM or RAL were investigated using high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS(2)), ultraperformance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), and Western blot analysis, respectively. TAM significantly increased the total EM and enhanced the formation of hormonally active and carcinogenic estrogen metabolites, 4-hydroxestrone (4-OHE1) and 16α-hydroxyestrone, with concomitant reduction in the formation of antiestrogenic and anticarcinogenic 2-hydroxyestradiol and 2-methoxyestradiol. Furthermore, TAM increased the formation of depurinating estrogen-DNA adducts 4-OHE1 [2]-1-N7Guanine and 4-OHE1 [2]-1-N3 Adenine. TAM-induced alteration in EM and depurinating DNA adduct formation is associated with altered expression of estrogen metabolizing enzymes CYP1A1, CYP1B1, COMT, NQO1, and SF-1 as revealed by Western blot analysis. In contrast to TAM, RAL has minimal effect on EM, estrogen-DNA adduct formation, or estrogen-metabolizing enzymes expression. These data show that TAM perturbs the balance of estrogen-metabolizing enzymes and alters the disposition of estrogen metabolites, which can explain, at least in part, the mechanism for TAM-induced EC. These results also implicate the differential effect of TAM and RAL on estrogen metabolism/metabolites as a potential mechanism for their disparate effects on the endometrium

Comparative Efficiency of Soil and Foliar Applied Zinc in Improving Yield and Yield Components of Wheat (Triticum aestivum L.) Variety Kiran-95

A field study was undertaken to evaluate the comparative efficacy of zinc application through soil and foliar spray on growth and yield of wheat variety Kiran-95 at Soil Chemistry Section, Agriculture Research Institute, Tandojam during Rabi 2013-14. The experiment was laid out in three replicated randomized complete block design. The treatments comprised Control (No Zinc), Soil applied Zinc 5.0 kg ha-1 at tillering stage, Soil applied Zinc 10.0 kg ha-1 at tillering stage, Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage, Foliar applied Zinc 0.2% at tillering stage, Foliar applied Zinc 0.4% at tillering and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage. The statistical analysis of data suggested that soil and foliar applied zinc affected significantly (P<0.05) growth and yield traits of wheat variety Kiran-95. The results illustrated that Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage produced maximum plant height (99.0 cm), tillers (410.7 m-2), spike length (13.1 cm), spikelets per spike (23.0), grains per  spike (45.1), seed index (43.4 g), biological yield (9354.4 kg ha-1) and grain yield (5123.4 kg ha-1), closely followed by Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage with 97.6 cm plant height, 408.8 tillers m-2, 13.0 cm spike length, 22.8 spikelets per spike, 45.0 grains per spike, 43.2 g seed index, 9273.4 kg ha-1 biological yield and 5080.7 kg ha-1 grain yield. The performance of wheat variety Kiran-95 ranked 3rd, 4th, 5th and 6th almost in all the growth and yield parameters particularly grain yield (kg ha-1) when fertilized with Soil applied Zinc 10.0 kg ha-1 at tillering stage, Foliar applied Zinc 0.4% at tillering, Soil applied Zinc 5.0 kg ha-1 at tillering stage and Foliar applied Zinc 0.2% at tillering stage.  However, minimum growth and yield traits were registered in Control (No Zinc). Furthermore, the results concluded that although numerically maximum growth and yield values were recorded in Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage but statistically the differences between Soil applied Zinc 5.0 kg ha-1 at tillering + 5.0 kg ha-1 at booting stage and Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage were non-significant. Hence, Foliar applied Zinc 0.2% at tillering stage + 0.2% at booting stage were found the most economical treatment for obtaining optimum yield of wheat variety Kiran-95. Keywords: Wheat, Comparative Efficiency, Soil, Foliar, Zinc, Improving Yield and Yield Component

Innovations In Formulation And Evaluation Of Oral Fast Dissolving Film

The most popular and convenient route for the drug administration is oral route with some merits, demerits, and limitations with some paediatric and geriatrics patients as they face difficulty to take solid dosage forms like capsules or compressed tablets that results in improper dosing due to vomit or removal of drug from buccal cavity. Oral fast dissolving films are a good alternate to overcome these difficulties and to deliver the drugs with any these age groups. Fast dissolving oral films are capable of delivering the drug locally and systemically by absorption through buccal mucosa, sublingual route, and oesophagus and finally form the stomach. Oral fast dissolving films offers a convenient way of dosing medication, not only for special groups of the population like paediatric, geriatric, confined to bed patients, patients with mental problems, but also to the general population. The present article review is focused on composition, preparation methods, evaluation and advancements of orally fast dissolving oral films

Catechol-O-Methyltransferase Expression and 2-Methoxyestradiol Affect Microtubule Dynamics and Modify Steroid Receptor Signaling in Leiomyoma Cells

CONTEXT: Development of optimal medicinal treatments of uterine leiomyomas represents a significant challenge. 2-Methoxyestradiol (2ME) is an endogenous estrogen metabolite formed by sequential action of CYP450s and catechol-O-methyltransferase (COMT). Our previous study demonstrated that 2ME is a potent antiproliferative, proapoptotic, antiangiogenic, and collagen synthesis inhibitor in human leiomyomas cells (huLM). OBJECTIVES: Our objectives were to investigate whether COMT expression, by the virtue of 2ME formation, affects the growth of huLM, and to explore the cellular and molecular mechanisms whereby COMT expression or treatment with 2ME affect these cells. RESULTS: Our data demonstrated that E(2)-induced proliferation was less pronounced in cells over-expressing COMT or treated with 2ME (500 nM). This effect on cell proliferation was associated with microtubules stabilization and diminution of estrogen receptor alpha (ERalpha) and progesterone receptor (PR) transcriptional activities, due to shifts in their subcellular localization and sequestration in the cytoplasm. In addition, COMT over expression or treatment with 2ME reduced the expression of hypoxia-inducible factor -1alpha (HIF-1 alpha) and the basal level as well as TNF-alpha-induced aromatase (CYP19) expression. CONCLUSIONS: COMT over expression or treatment with 2ME stabilize microtubules, ameliorates E(2)-induced proliferation, inhibits ERalpha and PR signaling, and reduces HIF-1 alpha and CYP19 expression in human uterine leiomyoma cells. Thus, microtubules are a candidate target for treatment of uterine leiomyomas. In addition, the naturally occurring microtubule-targeting agent 2ME represents a potential new therapeutic for uterine leiomyomas