3 research outputs found
Heterogeneous Fenton's-like catalysis for degradation of colchicine coupled with extraction of its biologically active metabolite
Work performed in Professor Huddersman's laboratory at De Montfort University by visiting Researcher (academic lecturer) from Faculty of Pharmacy, Assiut University, Egypt
The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.Nowadays, drug pollution; a form of water pollution caused by some pharmaceuticals and their metabolites resulting
from consumers, industry and hospitals was reported. Colchicine (CLN) is considered one of the pharmaceutical
wastewater contaminants which are not eliminated completely in municipal sewage treatment plants and are
discharged into receiving water. Due to the higher toxicity of CLN, a novel heterogeneous Fenton's-like catalysis was
established for complete degradation of CLN. So, a highly sensitive and specific liquid chromatographic method with
quadrupole mass spectrometry (LC/Q-MS) was developed and validated for estimation of CLN in its pure form and in
the presence of its degradation product. Herein, GraceSmart RP C18 column was utilized for separation of the cited
drug (Retention time tR= 5.578 min) using methanol: water (55: 45, v/v) at 1.0 mL/min. Detection was performed by
Agilent 6120 Quadrupole MS detector in a positive ionization mode. Thereafter and for the first time, degradation of
CLN by heterogeneous Fenton's-like catalysis using modified polyacrylonitrile (PAN) as a catalyst with H2O2 in
aqueous acidic medium was performed. This process was firstly optimized by HPLC/UV detection at 248 nm using the
aforementioned chromatographic conditions. As a result, CLN degraded completely within 30 min. The only observed
degradation product was the biologically active, potent and less toxic antitumor metabolite of CLN (3- demethyl CLN)
which was collected, extracted, and analyzed by Fourier Transfer- Infrared Spectroscopy (FTIR) and 13Carbon-
Nuclear Magnetic Resonance (13C-NMR). Finally, this method is eco-friendly and complies with the requirements of
the green chemistry. It is suitable for complete removal of CLN and/or its metabolite contaminants from wastewater
samples and estimation of the target drug without any interference from its degradation products. However, further
study is required to expand the method applicability to the pharmaceutical wastewater treatment as well the production
of 3- demethyl CLN on a large scale
Analysis for commonly prescribed non-sedating antihistamines
A comprehensive review with 185 references for the analysis of commonly prescribed members of an important class of drugs, non-sedating antihistamines (NSAs), is presented. The review covers most of the methods described for the analysis of cetirizine (CTZ), ebastine (EBS), fexofenadine (FXD), ketotifen (KET) and loratadine (LOR) in pure forms, in different pharmaceutical dosage forms and in biological fluids. The review covers the period from 1991 till now