Characterization of pyridine nucleotide binding site of UDP-glucose 4-epimerase from Saccharomyces fragilis

UDP-glucose 4-epimerase from Saccharomyces fragilis has 1 mol of NAD firmly bound per mol of the dimeric apoenzyme. This prevents a direct study of the coenzyme binding site of the protein. Dissociation of the dimer with p-chloromercuribenzoate and its reconstitution with exogenous NAD or one of its analogues and 2-mercaptoethanol provides an indirect method of study of the site. Depending on the reconstitution properties, the analogues can be classified in the following groups: (i) analogues that have no affinity for the site; (ii) analogues that have affinity but are not incorporated into the apoenzyme; (iii) analogues that produce catalytically inactive holoenzymes; and (iv) analogues that produce catalytically active holoenzymes. Minimum structural requirements that lead to affinity for the coenzyme site and to binding to the apoenzyme can also be discerned from these studies. Reconstitution with etheno-NAD, a fluorescent analogue of NAD, indicates the presence of a hydrophobic pocket for the adenosine subsite

Stability analysis of an eco-epidemiological model incorporating a prey refuge

The present paper deals with the problem of a predator-prey model incorporating a prey refuge with disease in the prey-population. We assume the predator population will prefer only infected population for their diet as those are more vulnerable. Dynamical behaviours such as boundedness, permanence, local and global stabilities are addressed. We have also studied the effect of discrete time delay on the model. The length of delay preserving the stability is also estimated. Computer simulations are carried out to illustrate our analytical findings

Violation of Bell's inequality for phase singular beams

We have considered optical beams with phase singularity and experimentally verified that these beams, although being classical, have properties of two mode entanglement in quantum states. We have observed the violation of Bell's inequality for continuous variables using the Wigner distribution function (WDF) proposed by Chowdhury et al. [Phys. Rev. A \textbf{88}, 013830 (2013)]. Our experiment establishes a new form of Bell's inequality in terms of the WDF which can be used for classical as well as quantum systems.Comment: 7 pages, 9 figures and 1 tabl

Non equilibrium statistical physics with fictitious time

Problems in non equilibrium statistical physics are characterized by the absence of a fluctuation dissipation theorem. The usual analytic route for treating these vast class of problems is to use response fields in addition to the real fields that are pertinent to a given problem. This line of argument was introduced by Martin, Siggia and Rose. We show that instead of using the response field, one can, following the stochastic quantization of Parisi and Wu, introduce a fictitious time. In this extra dimension a fluctuation dissipation theorem is built in and provides a different outlook to problems in non equilibrium statistical physics.Comment: 4 page

Inelastic scattering of protons from 6,8^{6,8}He and 7,11^{7,11}Li in a folding model approach

The proton-inelastic scattering from $^{6,8}$He and $^{7,11}$Li nuclei are studied in a folding model approach. A finite-range, momentum, density and isospin dependent nucleon-nucleon interaction (SBM) is folded with realistic density distributions of the above nuclei. The renormalization factors N$_R$ and N$_I$ on the real and volume imaginary part of the folded potentials are obtained by analyzing the respective elastic scattering data and kept unaltered for the inelastic analysis at the same energy. The form factors are generated by taking derivatives of the folded potentials and therefore required renormalizations. The $\beta$ values are extracted by fitting the p + $^{6,8}$He,$^{7,11}$Li inelastic angular distributions. The present analysis of p + $^8$He inelastic scattering to the 3.57 MeV excited state, including unpublished forward angle data (RIKEN) confirms L = 2 transition. Similar analysis of the p + $^6$He inelastic scattering angular distribution leading to the 1.8 MeV (L = 2) excited state fails to satisfactorily reproduce the data.Comment: one LaTeX file, five PostScript figure