Inelastic scattering of protons from 6,8^{6,8}He and 7,11^{7,11}Li in a folding model approach

The proton-inelastic scattering from $^{6,8}$He and $^{7,11}$Li nuclei are studied in a folding model approach. A finite-range, momentum, density and isospin dependent nucleon-nucleon interaction (SBM) is folded with realistic density distributions of the above nuclei. The renormalization factors N$_R$ and N$_I$ on the real and volume imaginary part of the folded potentials are obtained by analyzing the respective elastic scattering data and kept unaltered for the inelastic analysis at the same energy. The form factors are generated by taking derivatives of the folded potentials and therefore required renormalizations. The $\beta$ values are extracted by fitting the p + $^{6,8}$He,$^{7,11}$Li inelastic angular distributions. The present analysis of p + $^8$He inelastic scattering to the 3.57 MeV excited state, including unpublished forward angle data (RIKEN) confirms L = 2 transition. Similar analysis of the p + $^6$He inelastic scattering angular distribution leading to the 1.8 MeV (L = 2) excited state fails to satisfactorily reproduce the data.Comment: one LaTeX file, five PostScript figure

A facile quantitative assay for viral particle genesis reveals cooperativity in virion assembly and saturation of an antiviral protein

Conventional assays of viral particle assembly and release are time consuming and laborious. We have developed an enzymatic virus-like particle (VLP) genesis assay that rapid and quantitative and is also versatile and applicable to diverse viruses including HIV-1 and Ebola virus. Using this assay, which has a dynamic range of several orders of magnitude, we show that the efficiency of VLP assembly and release, i.e., the fraction of the expressed protein that is assembled into extracellular particles, is dependent on the absolute level of expression of either HIV-1 Gag or Ebola virus VP40. We also demonstrate that the activity of the antiviral factor tetherin is dependent on the level of HIV-1 Gag expression and the numbers of VLPs generated, and appears to become saturated as these parameters are increased

A Polymerase-chain-reaction Assay for the Specific Identification of Transcripts Encoded by Individual Carcinoembryonic Antigen (CEA)-gene-family Members

Carcinoembryonic antigen (CEA) is a tumor marker that belongs to a family of closely related molecules with variable expression patterns. We have developed sets of oligonucleotide primers for the specific amplification of transcripts from individual CEA-family members using the reverse transcriptase/ polymerase chain reaction (RT/PCR). Specific primer sets were designed for CEA, non-specific cross-reacting antigen (NCA), biliary glycoprotein (BGP), carcinoembryonic antigen gene-family members 1, 6 and 7 (CGMI, CGM6 and CGM7), and one set for all pregnancy-specific glycoprotein (PSG) transcripts. Primers were first tested for their specificity against individual cDNA clones and product-hybridization with internal, transcript-specific oligonucleotides. Total RNA from 12 brain and 63 gynecological tumors were then tested for expression of CEA-related transcripts. None were found in tumors located in the brain, including various mesenchymal and neuro-epithelial tumors. CEA and NCA transcripts were, however, present in an adenocarcinoma located in the nasal sinuses. In ovarian mucinous adenocarcinomas, we always found co-expression of CEA and NCA transcripts, and occasionally BGP mRNA. CEA-related transcripts were also found in some serous, endometrioid and clear-cell ovarian carcinomas. CEA, NCA and BGP transcripts were present in endometrial carcinomas of the uterus and cervical carcinomas, whereas uterine leiomyomas were completely negative. No transcripts were found from CGM 1, CGM6, CGM7 or from PSG genes in any of the tumors tested. The PCR data were compared with immunohistochemical investigations of ovarian tumors at the protein level using CEA (26/3/13)-, NCA-50/90 (9A6FR) and NCA-95 (80H3)-specific monoclonal antibodies

Application of Absorbing Boundary Condition to Nuclear Breakup Reactions

Absorbing boundary condition approach to nuclear breakup reactions is investigated. A key ingredient of the method is an absorbing potential outside the physical area, which simulates the outgoing boundary condition for scattered waves. After discretizing the radial variables, the problem results in a linear algebraic equation with a sparse coefficient matrix, to which efficient iterative methods can be applicable. No virtual state such as discretized continuum channel needs to be introduced in the method. Basic aspects of the method are discussed by considering a nuclear two-body scattering problem described with an optical potential. We then apply the method to the breakup reactions of deuterons described in a three-body direct reaction model. Results employing the absorbing boundary condition are found to accurately coincide with those of the existing method which utilizes discretized continuum channels.Comment: 21 pages, 5 figures, RevTeX

Correction : Control of PD-L1 expression by miR-140/142/340/383 and oncogenic activation of the OCT4-miR-18a pathway in cervical cancer.

This research was supported by a grant from the Department of Women’s Health Educational System, JSPS Grant-in-Aid for Scientific Research (C) (15K10697 and 16K11123) and the Science and Technology Planning Project of Guangdong Province, China (2014A020212124). We thank Dr. Zhujie Xu for experimental assistance. The authors declare that they have no conflict of interest.Peer reviewedPublisher PD

Transfer/Breakup Modes in the 6He+209Bi Reaction Near and Below the Coulomb Barrier

Reaction products from the interaction of 6He with 209Bi have been measured at energies near the Coulomb barrier. A 4He group of remarkable intensity, which dominates the total reaction cross section, has been observed. The angular distribution of the group suggests that it results primarily from a direct nuclear process. It is likely that this transfer/breakup channel is the doorway state that accounts for the previously observed large sub-barrier fusion enhancement in this system.Comment: 4 pages; 3 figure

Coulomb and nuclear breakup of 8^8B

The cross sections for the ($^8$B,$^7$Be-$p$) breakup reaction on $^{58}$Ni and $^{208}$Pb targets at the beam energies of 25.8 MeV and 415 MeV have been calculated within a one-step prior-form distorted-wave Born approximation. The relative contributions of Coulomb and nuclear breakup of dipole and quadrupole multipolarities as well as their interference have been determined. The nuclear breakup contributions are found to be substantial in the angular distributions of the $^7$Be fragment for angles in the range of 30$^\circ$ - 80$^\circ$ at 25.8 MeV beam energy. The Coulomb-nuclear interference terms make the dipole cross section larger than that of quadrupole even at this low beam energy. However, at the incident energy of 415 MeV, these effects are almost negligible in the angular distributions of the ($^7$Be-p) coincidence cross sections at angles below 4$^\circ$.Comment: Revised version, accepted for publication in Phys. Rev.

Effects of finite width of excited states on heavy-ion sub-barrier fusion reactions

We discuss the effects of coupling of the relative motion to nuclear collective excitations which have a finite lifetime on heavy-ion fusion reactions at energies near and below the Coulomb barrier. Both spreading and escape widths are explicitly taken into account in the exit doorway model. The coupled-channels equations are numerically solved to show that the finite resonance width always hinders fusion cross sections at subbarrier energies irrespective of the relative importance between the spreading and the escape widths. We also show that the structure of fusion barrier distribution is smeared due to the spreading of the strength of the doorway state.Comment: 13 pages, 3 figures, Submitted to Physical Review