Effect of Argania spinosa oil extract on proliferation and Notch1 and ERK1/2 signaling of T-cell acute lymphoblastic leukemia cell lines

The Argan tree, called Argania spinosa (L.) Skeels, is a tropical plant, which belongs to the Sapotaceae family, it is exploited essentially for its fruits. The endosperm seed of the fruit constitutes a good potential source of edible oil for human consumption and is endowed with important medicinal properties such as antioxidant, antimalarial and anti-proliferative. The aim of the present work is to evaluate the anti-proliferative effect of the oil extracted from seeds of A. spinosa in T-cell acute lymphoblastic leukemia human (T-ALL) context. The activity was assessed through an in vitro test on three T-ALL cell lines: JURKAT, MOLT3 and DND41. The cytotoxicity effects of A. spinosa oil extract were checked by MTT assay and the change in the activity levels of two T-ALL proliferation-related proteins (Notch1 and ERK) was investigated by Western blot, the results demonstrate that treatment with A. spinosa oil extract at the dose of 100 μg/mL inhibited the growth of JURKAT, MOLT3 and DND41 cells, and reduced the expression levels and the activity of proliferation-related proteins such as ERK1/2 and Notch1 intracellular domain. A. spinosa oil extract could be a potential preventive and therapeutic approach recommended as anti-proliferative against leukemia

ANTIOXIDANT AND THE IMMUNOMODULATORY ACTIVITIES EXHIBITED BY THREE PLANTS FROM LAMIACEAE FAMILY

Objective: The aim of this work was screening of the antioxidant activity and immunomodulatory effects of the crude extracts from the aerial parts of three Algerian endemic species from Lamiaceae.Methods: DPPH radical scavenging assay was used to find the antioxidant activity of Stachys circinata, Salvia verbenaca and Thymus guyonii and the immunostimulant potential of these plants extracts on the phagocytic activity which was measured by the carbon clearance rate test.Results: Our results obtained in this study shown that the IC50 of Thymus guyonii, Salvia verbenaca and Stachys circinata were 15,90;47,50 and 54,92 respectively. The phagocytic index was increased significantly in animals injected with Stachys circinata and Salvia verbenaca at doses of 200 mg/kg P≤0,05 but not significantly with Thymus guyonii when compared to the control group. The clearance rate of carbon was faster but not significantly P≥ 0,05 and the corrected phagocytic index α was increased at the group GIII with Salvia verbenaca but not significantly when compared with other groups P≥ 0,05.Conclusion: The Stachys circinata and Salvia verbenaca extracts proved an immune-stimulatory effect on the reticuloendothelial system and Thymus guyonii extract revealed anti-oxidant activity.Â

Hepatoprotective Role of Sodium Selenite Against Oxidative Damage Induced by Mercuric Chloride in Rat Albinos Wistar

Background: The present study was undertaken, to evaluate the protective effect of sodium selenite against mercuric chloride induced oxidative stress in experimental rats. Female Albinos Wistar rats randomly divided into four groups, were the first was served as a control, whereas the remaining groups respectively treated with: sodium selenite (1mg/ kg b.w; ip), mercuric chloride (1 mg/kg body weight i.p) and combination of sodium selenite and HgCl2. Change in liver enzyme activities, thiobarbituric acid reactive substances (TBARS) level, antioxidants and reduced glutathione (GSH) contents were determined after 10 days experimental period. Results: Exposure of rats to mercuric chloride caused a significant increase the lipid peroxidation level along with corresponding decrease in the reduced glutathione and various antioxidant enzymes in liver. And increase in serum: glucose level, APL and transaminases activities and decreased in total protein and albumin levels. Furthermore, treatment with mercuric chloride caused a marked elevation of liver weight and decreased body weight. Supplementation of sodium selenite resulted in decreased of lipid peroxidation level and in the serum: AST, ALT and APL activities were decreased along with increase in total protein, albumin and liver GSH levels. The activities of antioxidants enzymes: glutathione peroxidase (GSH -Px) and glutathione –S-transferase (GST) were also concomitantly restored to near normal level by sodium selenite supplementation to mercuric chloride intoxicated rats. Liver histological studies have confirmed the changes observed in biochemical parameters and proved the beneficial role of sodium selenite. Conclusion: The results clearly demonstrate that sodium selenite treatment augments the antioxidants defense mechanism in mercuric chloride induced toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases

Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats

Backgroud: Reactive oxygen species are known to play a major role in mercuric chloride induced oxidative and renal stress. Sodium selenite as an exogenous source of selenium is used for endogenous selenoprotein synthesis to scavenge the free radicals. The study was designed to investigate the possible protective role of sodium selenite in mercuric chloride induced renal stress, by using biochemical approaches. Adult male Albinos Wistar rats were randomly divided into four groups. The first group was served as the control, the second group was given sodium selenite (0.25 mg/kg b.w), while the third group was given mercuric chloride (0.25 mg/kg), finally, the fourth group was given combined treatment of sodium selenite and mercuric chloride for 3 weeks.Results: The effects of sodium selenite on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea, uric acid, billirubin levels and LDH activity, kidney tissue lipid peroxidation, GSH levels, GSH-Px, GST and catalase activities and hematological parameters. Administration of mercuric chloride induced significant increase in serum: creatinine, urea, uric acid and billirubin concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px, GST, and catalase activities along with increase the level of lipid peroxidation. Furthermore, treatment with mercuric chloride caused a marked elevation of kidney weight and decreased body weight and erythrocytes, hemoglobin, hematocrit levels. Sodium selenite treatment markedly reduced elevated serum: creatinine, urea, uric acid and billirubin levels, and LDH activity and conteracted the deterious effects of mercuric chloride on oxidative stress markers and hematological parameters and atteneuated histopathological changes caused by HgCl2 in kidney.Conclusion: Our results indicate that sodium selenite could have a beneficial role against mercuric chloride induced nephrotoxicity and oxidative stress in rat

THE PROTECTIVE EFFECTS OF ARGANIA SPINOSA SEEDS AGAINST HYPERHOMOCYSTENEMIA INDUCED BY HIGH METHIONINE DIET IN MICE

Objective : Hyperhomocysteinemia (HHcy), oxidative stress and decreased antioxidant capacities lead to several clinical manifestations and particularly, cardiovascular and liver diseases.  Our aim in this study was to investigate the protective effect of Argania spinosa crude exctract against high methionine diet  induced  HHcy ,  oxidative stress and damages in the aorta, and  heart of mice.Materials and methods: Adult male Mus Musculus were systematically divided into four groups of similar mean body weights and fed for 21 days with control and experimental diets. The control group (F) was fed with white bread (0.50mg/mice), group (M) was fed with L-methionine (500mg/kg/day), group (MP) was fed with L-methionine (500mg/kg/day) plus A.spinosa crude extract (150mg/kg), and the group (P) was treated with A.spinosa crude extract (150mg/kg/day). The experimental diets were given in white bread (0.50mg/mice). After 3 weeks of treatments, homocysteine (Hcy) concentrations, hepatic antioxidant status and histological sections of aorta and heart were determined.Results: Consumption of high methionine diet led to an increase in plasma Hcy , reduced the concentrations of GSH, and  the enzyme catalase. These were associated with the loss and degeneration of endothelium, fenestration and formation of foam cells of aorta, also the alteration of the cardiac muscle. However, administration of A.spinosa crude extract in combination  with methionine ameliorated all these changes.Conclusion:  A.spinosa crude extract is effective in decreasing plasma Hcy level as induced by methionine enriched diet in mice,   and improves the antioxidants defense

Immunostimulatory activity of <it>Stachys mialhesi</it> de Noé

Abstract Background Immunostimulatory therapy is now being recognized as an alternative to conventional chemotherapy for a variety of disease conditions, involving the impaired immune response of the host. In the present study, the immunostimulatory effect of the butanolic extract obtained from S. mialhesi aerial parts, was evaluated in vivo. Methods The immunostimulant potential of the plant extract on the phagocytic activity was measured by the carbon clearance rate test. Results Our research revealed that at different doses (50, 100 and 500 mg/kg), S. mialhesi extract increased the phagocytic activity in a dose dependant manner when compared with the control and thus the clearance rate of carbon was faster after the administration of the plant extract. Conclusion S. mialhesi extract exhibited a dose-dependent immunostimulant effect on the reticuloendothelial system, which could be attributed to the presence of active principles in this butanolic extract.</p

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ABSTRACT The study was designed to investigate the possible protective role of argan oil in mercuric chloride induced renal stress, by using biochemical approaches. The effects of argan oil on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea and uric acid levels, kidney tissue lipid peroxidation, GSH levels, GSH-Px and GST activities. Administration of mercuric chloride induced significant increase in serum: interleukine 1, interleukine 6 and tumor necrosis factor α (TNFα) levels, creatinine, urea and uric acid concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px and GST activities along with increase the level of lipid peroxidation. Argan oil treatment markedly reduced elevated serum: IL1, IL6, TNFα, creatinine, urea and uric acid levels and counteracted the deterious effects of mercuric chloride on oxidative stress markers changes caused by HgCl 2 in kidney. Our results indicate that argan oil could have a beneficial role against mercuric chloride induced nephrotoxicity and oxidative stress in rat

Total Phenols from Grape Leaves Counteract Cell Proliferation and Modulate Apoptosis-Related Gene Expression in MCF-7 and HepG2 Human Cancer Cell Lines

Grape leaves influence several biological activities in the cardiovascular system, acting as antioxidants. In this study, we aimed at evaluating the effect of ethanolic and water extracts from grape leaves grown in Algeria, obtained by accelerator solvent extraction (ASE), on cell proliferation. The amount of total phenols was determined using the modified Folin-Ciocalteu method, antioxidant activities were evaluated by the 2,2-diphenyl-l-picrylhydrazyl free radical (DPPH*) method and &#183;OH radical scavenging using electron paramagnetic resonance (EPR) spectroscopy methods. Cell proliferation of HepG2 hepatocarcinoma, MCF-7 human breast cancer cells and vein human umbilical (HUVEC) cells, as control for normal cell growth, was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay (MTT). Apoptosis- related genes were determined by measuring Bax and Bcl-2 mRNA expression levels. Accelerator solvent extractor yield did not show significant difference between the two solvents (ethanol and water) (p &gt; 0.05). Total phenolic content of water and ethanolic extracts was 55.41 &#177; 0.11 and 155.73 &#177; 1.20 mg of gallic acid equivalents/g of dry weight, respectively. Ethanolic extracts showed larger amounts of total phenols as compared to water extracts and interesting antioxidant activity. HepG2 and MCF-7 cell proliferation decreased with increasing concentration of extracts (0.5, 1, and 2 mg/mL) added to the culture during a period of 1&#8315;72 h. In addition, the expression of the pro-apoptotic gene Bax was increased and that of the anti-apoptotic gene Bcl-2 was decreased in a dose-dependent manner, when both MCF-7 and HepG2 cells were cultured with one of the two extracts for 72 h. None of the extracts elicited toxic effects on vein umbilical HUVEC cells, highlighting the high specificity of the antiproliferative effect, targeting only cancer cells. Finally, our results suggested that ASE crude extract from grape leaves represents a source of bioactive compounds such as phenols, with potential antioxidants activity, disclosing a novel antiproliferative effect affecting only HepG2 and MCF-7 tumor cells

Antioxidant, Anti-Inflammatory and Anti-Proliferative Properties of <i>Stachys circinata</i> on HepG2 and MCF7 Cells

According to the WHO, the overall age-standardized cancer rate keeps declining, and the number of cases diagnosed each year increases, remaining among the leading causes of death in 91 out of 172 recorded countries. In this context, novel cancer prediction and therapeutic protocols are compulsory. The effect of a Stachys circinata L’Hér dichloromethane extract (ScDME) on cell redox homeostasis and tumor proliferation was investigated. HepG2 cell feedback mechanisms to oxidative stress exposure were evaluated by determining catalase (CAT) and reduced glutathione (GSH), following the supply with ScDME (0.0–5.7 µg/µL). Cytotoxicity of ScDME against the human umbilical vein endothelial cell (HUVEC) and two human cancer cell lines (breast: MCF7; liver: HepG2) was evaluated by the MTT assay. H2O2-stressed HepG2 cells supplied with the S. circinata extracts exhibited significantly increased CAT and GSH activity as compared to unsupplied ones. The anti-inflammatory activity of the extracts was evaluated by real time-qPCR on IL-1, IL-6 and TNF-α expression. As a result, this research points out that S. circinata dichloromethane extract owns anti-inflammatory and anti-proliferative properties against MCF7 and HepG2 cells and activates CAT and GSH of the HepG2 cells’ antioxidant enzyme system

Compounds from the pods of Astragalus armatus with antioxidant, anticholinesterase, antibacterial and phagocytic activities

International audienceContext: The phytochemical study and biological activities of Astragalus armatus Willd. subsp. numidicus (Fabaceae) pods, an endemic shrub of Maghreb, are reported.Objective: This study isolates the secondary metabolites and determines the bioactivities of Astragalus armatus pods.Materials and methods: The chloroform, ethyl acetate and n-butanol extracts of hydro-ethanolic extracts were studied. Antioxidant activity was investigated using DPPH and ABTS radical scavenging, CUPRAC and ferrous chelating assays at concentrations ranging from 3 to 200 μg/mL. Anticholinesterase activity was determined against acetylcholinesterase and butyrylcholinesterase enzymes at 50, 100 and 200 μg/mL. Antibacterial activity was performed according to minimum inhibitory concentration (MIC) method. Carbon clearance method in albino mice was used for the phagocytic activity at concentrations 50, 70 and 100 mg/kg body weight. Spectroscopic techniques were used to elucidate the compounds.Results: Ethyl acetate extract afforded a flavonoid (1) while the n-butanol extract gave four flavonoids (2–5), a cyclitol (6) and a cycloartane-type saponin (7). The ethyl acetate extract exhibited highest antioxidant activity in DPPH (IC50: 67.90 ± 0.57 μg/mL), ABTS (IC50: 11.30 ± 0.09 μg/mL) and CUPRAC (A0.50: 50.60 ± 0.9 μg/mL) assays. The chloroform extract exhibited the best antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, each with 80 μg/mL MIC values. The n-butanol extract enhanced phagocytic activity.Discussion and conclusion: Isorhamnetin (1), isorhamnetin-3-O-α-l-rhamnopyranosyl-(1 → 6)-β-d-galactopyranoside (2), isorhamnetin-3-O-β-d-apiofuranosyl-(1 → 2)-[α-l-rhamnopyranosyl-(1 → 6)]-β-d-galactopyranoside (3), kaempferol-3-O-(2,6-di-O-α-l-rhamnopyranosyl)-β-d-galactopyranoside (4), kaempferol-3-O-(2,6-di-O-α-l-rhamnopyranosyl)-β-d-glucopyranoside (5), pinitol (6) and cyclomacroside D (7) were isolated whereas 1, 2, 6 and 7 are reported for the first time from A. armatus