LTP induction within a narrow critical period of immature stages enhances the survival of newly generated neurons in the adult rat dentate gyrus

Neurogenesis occurs in the adult hippocampus of various animal species. A substantial fraction of newly generated neurons die before they mature, and the survival rate of new neurons are regulated in an experience-dependent manner. Previous study showed that high-frequency stimulation (HFS) of perforant path fibers to the hippocampal dentate gyrus (DG) induces the long-term potentiation (LTP) in the DG, and enhances the survival of newly generated neurons in the DG. In this study, we addressed whether a time period exists during which the survival of new neurons is maximally sensitive to the HFS. We found that the enhancement of cell survival by HFS was exclusively restricted to the specific narrow period during immature stages of new neurons (7-10 days after birth). Furthermore, the pharmacological blockade of LTP induction suppressed the enhancement of cell survival by the HFS. These results suggest that the LTP induction within a narrow critical period of immature stages enhances the survival of newly generated neurons in rat DG

SIRIUS Project. IV. The formation history of the Orion Nebula Cluster driven by clump mergers

The Orion Nebula Cluster (ONC) is an excellent example for understanding the formation of star clusters. Recent studies have shown that ONC has three distinct age populations and anisotropy in velocity dispersions, which are key characteristics for understanding the formation history of the ONC. In this study, we perform a smoothed-particle hydrodynamics/$N$-body simulation of star cluster formation from a turbulent molecular cloud. In this simulation, stellar orbits are integrated using a high-order integrator without gravitational softening; therefore, we can follow the collisional evolution of star clusters. We find that hierarchical formation causes episodic star formation that is observed in the ONC. In our simulation, star clusters evolve due to mergers of subclumps. The mergers bring cold gas with the clumps into the forming cluster. This enhances the star formation in the cluster centre. The dense cold gas in the cluster centre continues to form stars until the latest time. This explains the compact distribution of the youngest stars observed in the ONC. Subclump mergers also contribute to the anisotropy in the velocity dispersions and the formation of runaway stars. However, the anisotropy disappears within 0.5 Myr. The virial ratio of the cluster also increases after a merger due to the runaways. These results suggest that the ONC recently experienced a clump merger. We predict that most runaways originated from the ONC have already been found, but walkaways have not.Comment: 15 pages, 21 figures, and 3 tables, accepted for MNRA

Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination

Clustered protocadherins (Pcdhs), a large group of adhesion molecules, are important for axonal projections and dendritic spread, but little is known about how they influence neuronal activity. The Pcdhβ cluster is strongly expressed in the hippocampus, and in vivo Ca2+ imaging in Pcdhβ-deficient mice revealed altered activity of neuronal ensembles but not of individual cells in this region in freely moving animals. Specifically, Pcdhβ deficiency increased the number of large-size neuronal ensembles and the proportion of cells shared between ensembles. Furthermore, Pcdhβ-deficient mice exhibited reduced repetitive neuronal population activity during exploration of a novel context and were less able to discriminate contexts in a contextual fear conditioning paradigm. These results suggest that one function of Pcdhβs is to modulate neural ensemble activity in the hippocampus to promote context discrimination

Orchestrated ensemble activities constitute a hippocampal memory engram

The brain stores and recalls memories through a set of neurons, termed engram cells. However, it is unclear how these cells are organized to constitute a corresponding memory trace. We established a unique imaging system that combines Ca2+ imaging and engram identification to extract the characteristics of engram activity by visualizing and discriminating between engram and non-engram cells. Here, we show that engram cells detected in the hippocampus display higher repetitive activity than non-engram cells during novel context learning. The total activity pattern of the engram cells during learning is stable across post-learning memory processing. Within a single engram population, we detected several sub-ensembles composed of neurons collectively activated during learning. Some sub-ensembles preferentially reappear during post-learning sleep, and these replayed sub-ensembles are more likely to be reactivated during retrieval. These results indicate that sub-ensembles represent distinct pieces of information, which are then orchestrated to constitute an entire memory

Primary omental Gastrointestinal stromal tumor (GIST)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

Targeting of ribosomal protein S6 to dendritic spines by in vivo high frequency stimulation to induce long-term potentiation in the dentate gyrus

Late phase long-term potentiation (L-LTP) in the hippocampus is believed to be the cellular basis of long-term memory. Protein synthesis is required for persistent forms of synaptic plasticity, including L-LTP. Neural activity is thought to enhance local protein synthesis in dendrites, and one of the mechanisms required to induce or maintain the long-lasting synaptic plasticity is protein translation in the dendrites. One regulator of translational processes is ribosomal protein S6 (rpS6), a component of the small 40S ribosomal subunit. Although polyribosomes containing rpS6 are observed in dendritic spines, it remains unclear whether L-LTP induction triggers selective targeting of the translational machinery to activated synapses in vivo. Therefore, we investigated synaptic targeting of the translational machinery by observing rpS6 immunoreactivity during high frequency stimulation (HFS) for L-LTP induction in vivo. Immunoelectron microscopic analysis revealed a selective but transient increase in rpS6 immunoreactivity occurring as early as 15 min after the onset of HFS in dendritic spine heads at synaptic sites receiving HFS. Concurrently, levels of the rpS6 protein rapidly declined in somata of granule cells, as determined using immunofluorescence microscopy. These results suggest that the translational machinery is rapidly targeted to activated spines and that this targeting mechanism may contribute to the establishment of L-LTP

Application of Micro-PIXE in Atmospheric Environmental Science Research:Elemental Maps in Root of Siebold\u27s Beech Seeding

Micro-beam scanning PIXE (micro-PIXE) and scanning transmisssion ion microscopy (STIM)were applied to measurement of one-year old seeding root from a Siebold\u27s beech(Fagus crenata Blume) tree. The beech seedling root samples were collected at the centerpiece of the Shirakami-Sanchi World Heritage Area during October 2001. Target samples for micro-PIXE and STIM analyses were transverse sections from tip, midpoint and root of the beech seedling root. We focused shedding light on tree root. STIM images were similar to those generated using stereomicroscope and/or transmission microscopy. Real images in the form of elemental maps were obtained for nine elements: Na, Mg, Al, Si, P, S, Cl, K, and Ca. Of these typical elemental maps were Si and Ca, which were both, concentrated in the epidermis. Si was prominent in the tip, while Ca was prominent in the root