Comparing Likert Scale Functionality Across Culturally and Linguistically Diverse Groups in Science Education Research: an Illustration Using Qatari Students’ Responses to an Attitude Toward Science Survey

Surveying is a common methodology in science education research, including cross-national and cross-cultural comparisons. The literature surrounding students’ attitudes toward science, in particular, illustrates the prevalence of efforts to translate instruments with the eventual goal of comparing groups. This paper utilizes survey data from a nationally representative cross-sectional study of Qatari students in grades 3 through 12 to frame a discussion around the adequacy and extent to which common adaptations allow comparisons to be made among linguistically or culturally different respondents. The analytic sample contained 2,615 students who responded to a previously validated 32-item instrument, 1,704 of whom completed the survey in Modern Standard Arabic and 911 in English. The purpose of using these data is to scrutinize variation in the performance of the instrument between groups of respondents as determined by language of survey completion and cultural heritage. Multi-group confirmatory factor analysis was employed to investigate issues of validity associated with the performance of the survey with each group, and to evaluate the appropriateness of using this instrument to make simultaneous comparisons across the distinct groups. Findings underscore the limitations of group comparability that may persist even when issues of translation and adaptation were heavily attended to during instrument development

Isoprenylation Inhibition Suppresses FcεRI-mediated Mast Cell Function and Allergic Inflammation

Allergic disease is driven by cell signaling cascades that activate immune cells. One key player is mast cells, which is activated by IgE antibodies signaling through the high affinity IgE receptor, FceRI. Therefore, targeting FceRI-mediated cascades can offer for novel treatments for allergic disease. Statins have been demonstrated to reduce the severity of asthma, a common allergic airway disease. Statins are an FDA approved class of drugs with the intended purpose of lowering blood cholesterol. We previously found that while statins inhibit mast cell function in allergic disease, these anti-inflammatory effects vary widely amongst differing mouse strains and human donors, suggesting genetic variability. This project sought to overcome statin resistance by acting “downstream” in the cholesterol synthesis pathway on protein isoprenylation pathways. The logic is that isoprenylated proteins are critical for FceRI signaling, thus blocking this step of protein modification should reduce FceRI-mediated mast cell function. The novel FGTI-2734 drug was used to suppress the isoprenylation enzymes farnesyl transferase and geranylgeranyl transferase. FGTI-2734 reduced IgE-mediated mast cell degranulation and cytokine and chemokine secretion. Additional work found that both transferases must be targeted to produce these anti-inflammatory effects. Furthermore, we revealed that the K-Ras protein is an isoprenylation target that is essential for IgE-mediated mast cell function. Collectively, these studies demonstrate the translational potential of the novel drug FGTI-2734 and suggest it acts by suppressing isoprenylation of proteins critical for mast cell function, including K-Ras.https://scholarscompass.vcu.edu/uresposters/1455/thumbnail.jp

Early postnatal genetic diagnosis for Joubert Syndrome Type 14

BACKGROUND: Joubert syndrome is an autosomal recessive condition characterised by a midbrain-hindbrain malformation giving rise to the characteristic “molar tooth sign” on MRI. Common signs and symptoms include neonatal breathing dysregulation, hypotonia and developmental delay. A spectrum of conditions referred to as Joubert syndrome and Related Disorders (JSRD) have also been described, referring to the presence of multiorgan involvement in addition to the neurological signs and symptoms characterising “pure Joubert”.CASE PRESENTATION: We report a case of JS type 14 (homozygous pathogenic variant in TMEM237) in a male neonate referred antenatally with a brain malformation and bilateral cystic kidneys on ultrasound and MRI, raising the suspicion of a ciliopathy. Clinical features at birth included hypotonia, hydrocephalus, ocular colobomas and breathing difficulties which required mechanical ventilation.CONCLUSION: Antenatal suspicion followed by confirmation of JS in the immediate postnatal period, has enabled early timely intervention by a multidisciplinary team, thus optimising parental counselling, developmental outcomes, and monitoring for potential future complications.peer-reviewe

A photograph of four orientalists (Bombay, 1885): knowledge production, religious identities, and the negotiation of invisible conflicts

Abstract By analyzing the history of a photograph taken in a Bombay photo studio in 1885, this article explores notions of the production of knowledge on India and cultural dialogues, encounters, appropriations, and conflicts in colonial British India in the late nineteenth century. The photograph was taken after a Hindu religious ceremony in honour of the Italian Sanskritist Angelo de Gubernatis. Dressed as a Hindu Brahman, he is the only European photographed next to three Indian scholars, but what the image suggests of encounter and hybridity was challenged by the many written texts that reveal the conflicting dialogues that took place before and after the portrait was taken. Several factors were examined in order to decide who should and who should not be in the photograph: religion, cast, and even gender were successively discussed, before the category of “knowledge” became the bond that unified the four men who studied, taught, and wrote on India

Alat Bantu Terapi Pasca Stroke untuk Tangan

Alat Bantu Terapi Pasca Stroke untuk Tangan adalah sebuah alat yang dirancang untuk memberikan kemudahkan kepada pasien penderita stroke dalam melakukan terapi mandiri, sehingga dapat mengurangi tingkat disabilitas pasca stroke. Dengan ketersediaan waktu yang tidak memadai untuk melakukan terapi di rumah sakit, pasien penderita stroke maupun keluarga pasien, kesulitan untuk menemukan jadwal terapi yang tersedia. Hal ini membuat keluarga sulit untuk menemukan jadwal untuk melakukan terapi dirumah sakit. Dengan alasan itulah dibuat sebuah Alat Bantu Terapi Pasca Stroke untuk Tangan yang dapat digunakan sebagai salah satu alat bantu dalam proses pemulihan pasca stroke yang dapat digunakan saat berada dirumah. Alat Bantu Pasca Stroke untuk Tangan ini dikontrol menggunakan mikrokontroler berbasis Mikrokontroler Arduino UNO dengan menggunakan motor DC sebagai penggeraknya untuk menahan beban tangan pasien. Alat ini dapat melakukan 4 (empat) mode pergerakan rehabilitasi, yaitu mode satu; gerakan siku kekiri dan kekanan, mode dua; gerakan siku keatas dan kebawah, mode tiga; lengan keatas dan kebawah, dan mode empat; gabungan dari semua mode dengan kecepatan yang aman bagi pasien pasca stroke. Tersedia mode pengaturan kecepatan pada alat, agar pengguna dapat mengatur kecepatan alat sesuai dengan kapasitasnya. Serta, pergerakan pada alat ini dapat memberikan kemudahan dan mengurangi resiko disabilitas

The thienopyridine A-769662 and benzimidazole 991 inhibit human TASK-3 potassium channels in an AMPK-independent manner

Heteromeric Tandem pore domain Acid Sensitive (TASK)-1/3 channels are critical to oxygen-sensing by carotid body type 1 cells, where hypoxia-induced inhibition of TASK-3 and/or TASK-1/3 potassium currents leads to voltage-gated calcium entry, exocytotic transmitter release and increases in carotid body afferent input responses that initiate corrective changes in breathing patterns. It was proposed that, in response to hypoxia, the AMP–activated protein kinase (AMPK) might directly phosphorylate and inhibit TASK channels, in particular TASK–3, but studies on rat type I cells questioned this view. However, sequence alignment identified a putative AMPK recognition motif in human (h) TASK-3, but not hTASK–1, with Ser55 representing a potential phosphorylation site. We therefore studied the effects of five different AMPK activators on recombinant hTASK–3 potassium channels expressed in human embryonic kidney (HEK)–293 cells. Two structurally unrelated AMPK activators, the thienopyridine A–769662 (100–500 µM) and the benzimidazole 991 (3–30 µM) inhibited hTASK–3 currents in a concentration–dependent manner, while the 4-azabenzimidazole MK–8722 (3–30 µM) partially inhibited hTASK–3 at concentrations above those required for maximal AMPK activation. By contrast, the 4-azabenzimidazole, BI-9774 (10–100 µM; a closely related analogue of MK8722) and the pro-drug AICA-riboside (1 mM; metabolised to ZMP, an AMP-mimetic) had no significant effect on hTASK–3 currents at concentrations sufficient to maximally activate AMPK. Importantly, A–769662 (300 µM) also inhibited hTASK–3 channel currents in HEK–293 cells that stably over-expressed an AMPK–β1 subunit mutant (S108A) that renders AMPK insensitive to activators that bind to the Allosteric Drug and Metabolite site, such as A–769662. We therefore identify A–769662 and 991 as novel hTASK–3 channel inhibitors and provide conclusive evidence that AMPK does not regulate hTASK–3 channel current

Everyday cosmopolitanism in representations of Europe among young Romanians in Britain

The paper presents an analysis of everyday cosmopolitanism in constructions of Europe among young Romanian nationals living in Britain. Adopting a social representations approach, cosmopolitanism is understood as a cultural symbolic resource that is part of everyday knowledge. Through a discursively-oriented analysis of focus group data, we explore the ways in which notions of cosmopolitanism intersect with images of Europeanness in the accounts of participants. We show that, for our participants, representations of Europe are anchored in an Orientalist schema of West-vs.-East, whereby the West is seen as epitomising European values of modernity and progress, while the East is seen as backward and traditional. Our findings further show that representations of cosmopolitanism reinforce this East/West dichotomy, within a discourse of ‘Occidental cosmopolitanism’. The paper concludes with a critical discussion of the diverse and complex ideological foundations of these constructions of European cosmopolitanism and their implications

The Value of MRI in Distinguishing Subtypes of Lipomatous Extremity Tumors Needs Reassessment in the Era of MDM2 and CDK4 Testing

Introduction. Extremity lipomas and well-differentiated liposarcomas (WDLs) are difficult to distinguish on MR imaging. We sought to evaluate the accuracy of MRI interpretation using MDM2 amplification, via fluorescence in-situ hybridization (FISH), as the gold standard for pathologic diagnosis. Furthermore, we aimed to investigate the utility of a diagnostic formula proposed in the literature. Methods. We retrospectively collected 49 patients with lipomas or WDLs utilizing MDM2 for pathologic diagnosis. Four expert readers interpreted each patient\u27s MRI independently and provided a diagnosis. Additionally, a formula based on imaging characteristics (i.e. tumor depth, diameter, presence of septa, and internal cystic change) was used to predict the pathologic diagnosis. The accuracy and reliability of imaging-based diagnoses were then analyzed in comparison to the MDM2 pathologic diagnoses. Results. The accuracy of MRI readers was 73.5% (95% CI 61-86%) with substantial interobserver agreement (κ = 0.7022). The formula had an accuracy of 71%, which was not significantly different from the readers (p = 0.71). The formula and expert observers had similar sensitivity (83% versus 83%) and specificity (64.5% versus 67.7%; p = 0.659) for detecting WDLs. Conclusion. The accuracy of both our readers and the formula suggests that MRI remains unreliable for distinguishing between lipoma and WDLs

A multi-disciplinary approach to the diagnosis and management of allergic diseases:An EAACI Task Force

Background: Guidelines for management of patients with allergic conditions are available, but the added value of nurses, allied healthcare professionals (AHPs), and general practitioners (GPs), in the management of allergic disease, has not been fully clarified. The European Academy of Allergy and Clinical Immunology (EAACI) appointed a task force to explore this issue. Aim To investigate the added value of nurses, AHPs, and GPs in management of allergic diseases, in an integrated model of care. Methods: A search was made of peer-reviewed literature published between 2010 and December 2020 (Cochrane Library, PubMed, and CINAHL) on the involvement of the various specific healthcare providers (HCPs) in the management of allergic diseases. Results: Facilitative models of care for patients with allergies can be achieved if HCP collaborates in the diagnosis and management. Working in multi-disciplinary teams (MDT) can increase patients' understanding of the disease, adherence to treatment, self-care capabilities, and ultimately improve quality of life. The MDT competencies and procedures can be improved and enhanced in a climate of mutual respect and shared values, and with inclusion of patients in the planning of care. Patient-centered communication among HCPs and emphasis on the added value of each profession can create an effective integrated model of care for patients with allergic diseases. Conclusion: Nurses, AHPs, and GPs, both individually and in collaboration, can contribute to the improvement of the management of patients with allergic disease. The interaction between the HCPs and the patients themselves can ensure maximum support for people with allergies