A comprehensive review on electrospinning design, parameters and potential use of electrospun nanofibers in regenerative endodontics

Electrospinning is a versatile technique that has gathered interest due to its ability to fabricate nano and microscale fibres with unique properties of high surface area and fibrous porosity. This technique has been widely used in the late 20th (1990) and early 21st (2000) centuries. Since the beginning of its use, significant improvements have been made in the design, materials used, and fibres produced. The electrospinning technique is used to fabricate a material with therapeutic properties as it allows the researchers to incorporate various anti-microbial agents to different polymers without altering the chemical characteristicsof polymers.The production of nanofibres through electrospinning is affected by many operating parameters. It is, therefore, essential to know various parameters and processes that aid in fabricating the desired fibre assemblies. The nanofibres remain an essential division of biomaterials due to a wide range of biomedicalapplications. Nanofibres have unique properties such as protein absorption, binding sites to cell receptors, can provide maximum volume fraction by controlling fibres\u27 alignment and orientation hence improving the material properties like surface morphology, porosity, and geometry.Recent trends in endodontics, encourage regenerative therapy for the treatment of necrotic immature permanent teeth for root development and maturation. In this context, efficient disinfection of the root canal system is a crucial step. Existing chemical irrigating solutions (for eg., NaOCl) and antibiotic pastes (for eg., Triple antibiotic paste) usage at higher doses showed toxic results on the pulpal stem cells. Therefore, it was found to be beneficial to use a nanofibre-based intracanal drug delivery construct to release antibiotics at lower, yet anti-microbially effective concentrations.This review aims to discuss the basic concepts of electrospinning and its potential application in regenerative endodontics along with various parameters, which affect the fibre morphology and properties of produced nanofibres

In vitro evaluation of the quality of obturation with capillary condensation technique and hybrid technique in teeth with simulated internal resorption cavity: A cone-beam computed tomography study

Aim: The quality of obturation with recent capillary condensation technique and hybrid technique in teeth with artificial internal resorption cavity needs to be evaluated. Methods: Calculation of sample size was done with G*Power software (version 3.1.9.4) (effect size d = 1.46). Eighteen single-canal mandibular premolars were decoronated at the cementoenamel junction for standardization of specimen length as 12 mm. Biomechanical preparation was done with ProTaper Universal rotary file up to F4. Internal resorption cavity was prepared with #08 size round bur 6 mm from the apex. Samples were distributed randomly into two groups. Group I: teeth with bioceramic sealer (EndoSequence BC Sealer, Brasseler, USA) application using conventional technique and hybrid technique of obturation. Group II: capillary condensation technique of bioceramic sealer (EndoSequence BC Sealer, Brasseler USA) using single-cone gutta-percha obturation. The voids in three segments of the root canal were measured with cone-beam computed tomography using “OnDemand3D App” software. Data were statistically analyzed by one-way analysis of variance (ANOVA) and multiple comparisons of Tukey honestly significant difference tests with P < 0.05. Results: The mean area of the void was 0.3963 ± 0.3299 mm2 for Group I and 0.4022 ± 0.4101 mm2 for Group II. There was a difference in the number of voids present in different sections of the root within a group with significance but not between the groups. Conclusion: Capillary condensation technique with single-cone obturation is comparable to conventional sealer application and hybrid technique of obturation for filling internal resorption cavity

Oral health status, treatment needs, and patterns of utilization of dental services among village volunteers in Andhra Pradesh: A cross-sectional multi-stage cluster survey

Background: Community health depends on the leadership duties of a diverse population, such as village volunteers. Hence, a study was conducted to assess oral health status, treatment needs, and patterns of utilization of dental services among village volunteers in Andhra Pradesh state. Methodology: A cross-sectional study was conducted among 400 village volunteers in Andhra Pradesh state, India. A multi-stage cluster sampling procedure was employed in sample selection. A questionnaire was used to review the pattern of utilization of dental services. World Health Organization Oral Health assessment form 1997 was used to evaluate oral health status and treatment needs. Data collected were analyzed using IBM SPSS Statistics for Windows, Version 25.0. Descriptive statistics were done. Results: Out of 400, 18% had never visited the dental clinic in their lifetime. The majority (33.5%) considered that dental problems were not a serious concern. Dental caries was seen in 69.5%, and 78.5% were presented with periodontal conditions. About 19.8% and 24.3% had crowding and spacing in incisal segments, respectively. The prevalence of oral mucosal disorders and dental fluorosis was 4.25% and 8.75%, respectively. Overall, 94% of the study participants needed dental treatment for various reasons. Conclusion: This study provides sufficient evidence to conclude that this population's oral health was poor, with increasing unmet dental treatment needs. Selected interventions and strategies should focus on these factors to decrease the burden of oral diseases among village volunteers

Characteristics of endodontic clinical trials registered in clinicalTrials.gov between 2000 and 2022

Aim: This study aims to examine the characteristics of endodontic clinical trials registered in the ClinicalTrials.gov database. Methods: The ClinicalTrials.gov website was searched for endodontic clinical trials that were registered between 2000 and 2022. The search term used was “endodontics.” As a result of the search strategy, 613 trials were identified. Out of which, 500 clinical trials met our inclusion criteria. For each clinical trial, information was extracted including the year of registration, location of the trial, type of trial, study design (allocation, intervention model, and masking) estimated enrollment, participants' age, health condition/problems studied, intervention/treatment, the status of the trial, study results, phase of the trial, and source of funding. Fisher's exact test was applied to identify the characteristics of clinical trials associated with funding. Results: The number of registered clinical trials gradually increased from 2000 to 2022. Out of 500 clinical trials, 456 were interventional, and 44 were observational. Only 18 clinical trials had secured funding. The majority of interventional trials (90.8%) are randomized, and the most common interventional model was parallel assignment (88.4%). It was observed that irreversible pulpitis was the most common condition studied (32.2%). Nonsurgical endodontic therapy was the most common intervention used in the trials (42.2%). Conclusion: The study provided an overview of endodontic clinical trials that were registered in the ClinicalTrials.gov. The findings showed that registered endodontic clinical trials have increased rapidly since 2000. The vast majority of trials took place in Africa. The majority of endodontic clinical trials followed rigorous methods in terms of allocation, intervention model, and masking. However, only a small portion of trials had secured funding. The findings also indicate the need for trial results to be made publicly available to enhance evidence-based practice in endodontics