214 research outputs found
Regulation of antioxidant enzyme activities in female rat brain by ovarian steroids
The sex steroids produce their effects by acting on numerous target tissues and organs, such as the reproductive organs, bone tissue and cartilage, peripheral blood vessels as well as the central nervous system (CNS). In our studies we have monitored the change of enzyme activity of the antioxidant (AO) system in the brain of female rats depending on the ovarian steroids. We have chosen it as a new parameter that might represent an important indicator of the changes within the CNS, bearing in mind the biological importance of the enzymes of the AO system. The experimental results of our study indicate that the enzyme activity of the AO system in the brain tissue of female rats shows a certain dependence on the concentration of ovarian hormones, progesterone and estradiol, in the organism. Study of the activity of the enzymes of the AO system in the brain of female rats depending on the influence of ovarian hormones can answer whether the action of ovarian steroids on the CNS includes maintenance of a dynamic equilibrium of free radicals in the neurons
Activities of antioxidant defense enzymes in the blood of individuals with Leu144Phe mutation
Activities of cooper zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of familial amyotrophic lateral sclerosis patients with Leu144Phe mutation (FALS), asimptomatic carriers with Leu144Phe mutation and controls were studied. Activity of Cu,Zn SOD was significantly lower in the FALS patients and asimptomatic carriers than in controls (p<0.001). In the FALS patients GSH-Px activity was lower (p<0.01) and activity of GR was higher (p<0.001) in comparison with controls. Canonical discriminant analyses provide statistical evidence that examined groups are different in the composition of antioxidant enzymes in blood and revealed that each component confers to observed difference. Our results suggests that oxidative stress is involved in pathogenesis of FALS and the activities of antioxidant enzymes are exposed to different kind of oxidative pressure in FALS patients, asymptomatic carriers and controls.U ovom radu ispitivana je aktivnost: bakar cink sadržavajuÄe superoksid dismutaze (Cu, Zn SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation-S-transferaze (GST) u krvi pacijenata sa familijarnim oblikom amiotrofiÄne lateralne skleroze (FALS) sa mutacijom Leu144Phe, asimptomskim nosiocima mutacije Leu144Phe i kontrola. Aktivnost Cu,Zn SOD je statistiÄki znaÄajno niža kod FALS pacijenata i asimptomskih nosioca mutacije Leu144Phe nego kod kontrola (p < 0,001). Kod FALS pacijenata aktivnost GSH-Px je niža (p < 0,01), a aktivnost GR je veÄa (p < 0,001) u poreÄenju sa kontrolnom grupom. Kanonijska diskriminantna analiza obezbeÄuje statistiÄku podrÅ”ku uoÄene razlike u sastavu antioksidacionih zaÅ”titnih enzima u krvi ispitivanih grupa i pokazuje nam da svaka komponenta znaÄajno doprinosi toj razlici. NaÅ”i rezultati sugeriÅ”u da je oksidacioni stres ukljuÄen u patogenezu FALS i da su antioksidacioni zaÅ”titni enzimi izloženi razliÄitom oksidacionom pritisku kod FALS, asimptomskih nosioca mutacije Leu144Phe i kontrola.nul
Activities of antioxidant defense enzymes in the blood of individuals with Leu144Phe mutation
Activities of cooper zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of familial amyotrophic lateral sclerosis patients with Leu144Phe mutation (FALS), asimptomatic carriers with Leu144Phe mutation and controls were studied. Activity of Cu,Zn SOD was significantly lower in the FALS patients and asimptomatic carriers than in controls (p<0.001). In the FALS patients GSH-Px activity was lower (p<0.01) and activity of GR was higher (p<0.001) in comparison with controls. Canonical discriminant analyses provide statistical evidence that examined groups are different in the composition of antioxidant enzymes in blood and revealed that each component confers to observed difference. Our results suggests that oxidative stress is involved in pathogenesis of FALS and the activities of antioxidant enzymes are exposed to different kind of oxidative pressure in FALS patients, asymptomatic carriers and controls.U ovom radu ispitivana je aktivnost: bakar cink sadržavajuÄe superoksid dismutaze (Cu, Zn SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation-S-transferaze (GST) u krvi pacijenata sa familijarnim oblikom amiotrofiÄne lateralne skleroze (FALS) sa mutacijom Leu144Phe, asimptomskim nosiocima mutacije Leu144Phe i kontrola. Aktivnost Cu,Zn SOD je statistiÄki znaÄajno niža kod FALS pacijenata i asimptomskih nosioca mutacije Leu144Phe nego kod kontrola (p < 0,001). Kod FALS pacijenata aktivnost GSH-Px je niža (p < 0,01), a aktivnost GR je veÄa (p < 0,001) u poreÄenju sa kontrolnom grupom. Kanonijska diskriminantna analiza obezbeÄuje statistiÄku podrÅ”ku uoÄene razlike u sastavu antioksidacionih zaÅ”titnih enzima u krvi ispitivanih grupa i pokazuje nam da svaka komponenta znaÄajno doprinosi toj razlici. NaÅ”i rezultati sugeriÅ”u da je oksidacioni stres ukljuÄen u patogenezu FALS i da su antioksidacioni zaÅ”titni enzimi izloženi razliÄitom oksidacionom pritisku kod FALS, asimptomskih nosioca mutacije Leu144Phe i kontrola.nul
Glutathione as a suitable biomarker in hepatopancreas, gills and muscle of three freshwater crayfish species
We determined the contents of total glutathione (tGSH), reduced glutathione (GSH), and oxidized glutathione (GSSG) and values of the glutathione redox index (GSH RI) in hepatopancreas, gills, and muscle of three freshwater crayfish species: noble crayfish (Astacus astacus) from the Southern Morava River, stone crayfish (Austropotamobius torrentium) from the KrajkovaÄka River, and spinycheek crayfish (Orconectes limosus) from the Danube River. The obtained data show strong tissue and species specifity of investigated parameters: tGSH, GSH, GSSG, and GSH RI in the hepatopancreas, gills, and muscle of the indicated crayfish species. Our work represents the first study of its kind and showed that the investigated parameters can be considered suitable biomarkers of the cellular glutathione redox status in of freshwater crayfish species.OdreÄivan je sadržaj ukupnog glutationa (tGSH),redukovanog glutationa (GSH),oksidovanog glutationa (GSSG)i glutation redoks indeksa (GSH RI)u hepatopankreasu, Å”krgama i miÅ”iÄima tri vrste slatkovodnih rakova: reÄnog raka (Astacus astacus) iz reke Južna Morava, raka kamenjara (Austropotamobius torrentium) iz KrajkovaÄke reke i ameriÄkog raka (Orconecteslimosus) iz reke Dunav. Dobijeni podaci pokazuju znaÄajnu tkivnu i specijes specifiÄnost ispitivanih parametara: GSH, GSSG i GSHRI u hepatopankreasu, Å”krgama i miÅ”iÄima kod rakova. NaÅ” rad predstavlja prvu studiju ove vrste i pokazuje da ispitivani parametri mogu biti pogodni biomarkeri Äelijskog redoks statusa glutationa kod slatkovodnih vrsta rakova.Projekat ministarstva br. 143035
Results of the 2006 Sava survey: Aquatic macroinvertebrates
The paper presents some results of the 2006 Sava survey. The investigation was carried out at four locations along 188 km of the Serbian stretch of the Sava River (206 km of the river belongs to Serbia). Among other things (physical and chemical properties of water and sediment, phytoplankton, and phytobenthos), the study included investigation of aquatic macroinvertebrates. Sixty-two taxa were identified in this typical lowland river. Mollusks and oligochaetes were the most diverse groups of macroinvertebrates. Our results support the hypothesis that the Sava River is an important bio-invasion trajectory, a part of the Southern Invasive Corridor of Europe. Five alien macroinvertebrate taxa were identified, some of which (Corbicula fluminea, Branchyura sowerbyi, and Anodonta woodiana) were found to be important components of the community.Cilj rada je da se prikaže deo rezultata istraživanja na reci Savi tokom 2006. Istraživanje je vrÅ”eno na Äetiri lokaliteta duž 188 km reÄnog toka Save kroz Srbiju (od 206 km ukupne dužine toka). Pored istraživanja ostalih parametara (analiza fiziÄkih i hemijskih karakteristika vode, sedimenta, fitoplanktona i fitobentosa) vrÅ”ena su i istraživanja akvatiÄnih makroinvertebrata. Ukupno je zabeleženo 62 taksona. Najraznovrsnije grupe su Oligochaeta i Mollusca. Dobijeni rezultati potvrÄuju pretpostavku da je reka Sava važan invazivni put i da predstavlja deo južnoevropskog invazivnog koridora. Ukupno je zabeleženo 5 alohtonih vrsta makroinvertebrata, od kojih neke pred stavljaju važne komponente zajednice (Corbicula fluminea, Branchyura sowerbyi, i Anodonta woodiana).Projekat ministarstva br. 143023B i 143035
The effect of cadmium on ascorbic acid, vitamin E and coenzyme Q concentrations in rat kidneys: A possible protective role of coenzyme Q10
Projekat ministarstva br. 03E18 i 03E2
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